Imaging life from Molecules to cells - building knowledge on Cryo-electron microscopy methodologies

Información del proyecto

IMpaCT

Identificador del acuerdo de subvención: 857203

DOI

10.3030/857203

Proyecto cerrado

Fecha de la firma de la CE 9 Julio 2019

Fecha de inicio 1 Septiembre 2019

Fecha de finalización 28 Febrero 2023

Financiado con arreglo a

• Twinning of research institutions

Coste total

€ 800 000,00

Aportación de la UE

€ 800 000,00

Coordinado por

UNIVERSIDADE NOVA DE LISBOA
Portugal

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (7)

D4.5 Report on Dissemination, Communication & Exploitation activities

Different activities are planned for dissemination Thus a report will be prepared by the end of the project reporting all the different activities performed during the project

D2.6 Part II - Scientific seminars from international researchers on Cryo-EM areas

A total of 2 seminars per year will be organized at ITQB NOVA to be presented by scientists well established in the field The selection of speakers will consider the different methodologies of the CryoEM technology and as much as possible cover research scientific projects overlapping those of the MX Unit One of the goals will be to bring scientists recognized as landmarks in the CryoEM methodologies for instance those that were awarded the 2017 Nobel Prize of Chemistry This deliverable concerns to the second part of these seminars

D2.3 Part I - Scientific seminars from international researchers on Cryo-EM areas

A total of 2 seminars per year will be organized at ITQB NOVA to be presented by scientists well established in the field The selection of speakers will consider the different methodologies of the CryoEM technology and as much as possible cover research scientific projects overlapping those of the MX Unit One of the goals will be to bring scientists recognized as landmarks in the CryoEM methodologies for instance those that were awarded the 2017 Nobel Prize of Chemistry This deliverable concerns to the first part of these seminars

D 4.3 Reports on the Scientific conferences participation

Dissemination will be achieved mainly through participation in scientific conferences and thematic workshops and publishing of the results in high impact specialized peerreviewed journals We will aim to participate in scientific conferences specific to the different research projects that are ongoing in our MX Unit namely cancer viral infections and cellular substructures results of the different projects with major contributions directly arising from the shortterm visits to the Widening partners will be especially highlighted

D3.2 Plan of the career development short courses for early-stage researchers

A devoted WP (WP3) to the career development of early-stage researchers are planned within the proposal. The activities planned are training session regarding grant and scientific writing; communicating science, and IPR training. Thus a detail plan of the career development short courses for early-stage researchers will be performed based on the discussions that will occur during the kick-off-meeting.

D4.4 Participant evaluation of the Mini-symposium Pharmaceutical Cryo-EM applications

Our exploitation strategy will include the organization of a minisymposium targeted at pharmaceutical companies to raise awareness to the potential contribution of CryoEM to drug discovery and development pipelines One of the goals will be to collect the views and ideas of the industrial participants about their needs and problems to which science may provide solutions Different pharmaceutical companies will be invited to participate those that have already experience in CryoEM methodologies and others that have not started their studies using CryoEM The impact will be evaluated based on the quiz to the participants and on the diversity of the different pharmaceutical companies that will participate in the minisymposium

D 4.2 Outreach activities

Outreach activities will be promoted for the general public but also devoted to students from high school undergraduate master and doctoral levels The goal is to communicate the vast implications of Structural Biology by setting the basis for a twoway dialogue with target audiences increase the visibility and reputation of the project and partners perhaps also encouraging some of the young people to consider careers in science

Publicaciones

Peer reviewed articles (43)

Improvements on marker-free images alignment for electron tomography

Autores: C.O.S. Sorzano, F. de Isidro-Gómez, E. Fernández-Giménez, D. Herreros, S. Marco, J.M. Carazo, C. Messaoudi.
Publicado en: Journal of Structural Biology X, Edición 4, 2020, Página(s) 137, ISSN 2590-1524
Editor: Elsevier
DOI: 10.1016/j.yjsbx.2020.100037

The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stress

Autores: Sandra P. Santos, Yang Yang, Margarida T. G. Rosa, Mafalda A. A. Rodrigues, Claire Bouthier De La Tour, Suzanne Sommer, Miguel Teixeira, Maria A. Carrondo, Peter Cloetens, Isabel A. Abreu, Célia V. Romão
Publicado en: Scientific Reports, Edición 9/1, 2019, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-019-53140-2

Studies of multifunctional DNA polymerase I from the extremely radiation resistant Deinococcus radiodurans: recombinant expression, purification and characterization of the full-length protein and its large fragment

Autores: Fernandes, A., Piotrowski, Y., Williamson, A. Frade, K, and Moe, E.
Publicado en: Protein Expression and Purification, 2021, Página(s) 105925, ISSN 1046-5928
Editor: Academic Press
DOI: 10.1016/j.pep.2021.105925

In vitro neutralisation of Zika virus by an engineered protein targeting the viral envelope fusion loop

Autores: Isabelle F. T. Viana, Carlos H. B. Cruz, Diogo Athayde, W. Camilla S. Adan, Lícya S. S. Xavier, Margarida Archer and Roberto D. Lins
Publicado en: Molecular Systems Design & Engineering, Edición 8, 2023, Página(s) 516-526, ISSN 2058-9689
Editor: Royal Society of Chemistry
DOI: 10.1039/d2me00170e

Algorithmic robustness to preferred orientations in Single Particle Analysis by CryoEM

Autores: C.O.S. Sorzano, D. Semchonok, S.C. Lin, J.L. Vilas, A. Jimenez-Moreno, M. Gragera, D. Maluenda, M. Martinez, E. Ramirez-Aportela, R. Melero, A. Cuervo, P. Conesa, L. del Caño, Y.C. Fonseca, R. Sánchez-García, D. Strelak, E. Fernández-Giménez, F. de Isidro, P. Kastritis, R. Marabini, B. Bruce, J.M. Carazo.
Publicado en: Journal of Structural Biology, Edición 213 / 1, 2021, Página(s) 107695, ISSN 1047-8477
Editor: Academic Press
DOI: 10.1016/j.jsb.2020.107695

On bias, variance, overfitting, gold standard and consensus in single-particle analysis by cryo-electron microscopy

Autores: C.O.S. Sorzano, A. Jimenez-Moreno, D. Maluenda, M. Martinez, E. Ramirez-Aportela, R. Melero, A. Cuervo, J. Conesa, J. Filipovic, P.Conesa, L. del Caño, Y.C. Fonseca, J. Jiménez-de la Morena, P. Losana, R. Sáchez-García, D. Strelak, E. Fernandez-Gimenez, F. de Isidro, D.Herreros, J.L. Vilas, R. Marabini, J.M. Carazo
Publicado en: Acta Crystallographica Section D, Edición 78 / 4, 2022, Página(s) 410-423, ISSN 2059-7983
Editor: International Union of Crystallography
DOI: 10.1107/s2059798322001978

Identification of a conserved virion-stabilizing network inside the interprotomer pocket of enteroviruses

Autores: Flatt, J.W., Domanska, A., Seppälä, A.L., Butcher, S.J.
Publicado en: Communications Biology, Edición 4, 2021, Página(s) 250, ISSN 2399-3642
Editor: SpringerNature
DOI: 10.1038/s42003-021-01779-x

SARS-CoV-2 production, purification methods and UV inactivation for proteomics and structural studies

Autores: Plavec, Z., Domanska, A., Liu, X., Laine, P., Paulin, L., Varjosalo, M., Auvinen, P., Wolf, S.G., Anastasina, M., Butcher, S.J.
Publicado en: Viruses, Edición 14 / 9, 2022, Página(s) 1989, ISSN 1999-4915
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14091989

Sweet and sour: an update on classic galactosemia

Autores: Ana I. Coelho, M. Estela Rubio-Gozalbo, João B. Vicente, Isabel Rivera
Publicado en: Journal of Inherited Metabolic Disease, Edición 40/3, 2017, Página(s) 325-342, ISSN 0141-8955
Editor: Kluwer Academic Publishers
DOI: 10.1007/s10545-017-0029-3

Syntheses of the plant auxin conjugate 2- O -(indole-3-acetyl)- myo -inositol IAInos

Autores: Saúl Silva, Osvaldo S. Ascenso, Eva C. Lourenço, Margarida Archer, Christopher D. Maycock, M. Rita Ventura
Publicado en: Organic & Biomolecular Chemistry, Edición 16/38, 2018, Página(s) 6860-6864, ISSN 1477-0520
Editor: Royal Society of Chemistry
DOI: 10.1039/c8ob02096e

SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions

Autores: Sandra P. Santos, Maxime G. Cuypers, Adam Round, Stephanie Finet, Theyencheri Narayanan, Edward P. Mitchell, Célia V. Romão
Publicado en: Journal of Molecular Biology, Edición 429/5, 2017, Página(s) 667-687, ISSN 0022-2836
Editor: Academic Press
DOI: 10.1016/j.jmb.2017.01.008

Hydrogen Sulfide Oxidation: Adaptive Changes in Mitochondria of SW480 Colorectal Cancer Cells upon Exposure to Hypoxia

Autores: Francesca Malagrinò, Karim Zuhra, Ludovica Mascolo, Daniela Mastronicola, João B. Vicente, Elena Forte, Alessandro Giuffrè
Publicado en: Oxidative Medicine and Cellular Longevity, Edición 2019, 2019, Página(s) 1-11, ISSN 1942-0900
Editor: Landes Bioscience
DOI: 10.1155/2019/8102936

X-ray structure of full-length human RuvB-Like 2 – mechanistic insights into coupling between ATP binding and mechanical action

Autores: Sara T. N. Silva, José A. Brito, Rocío Arranz, Carlos Óscar S. Sorzano, Christine Ebel, James Doutch, Mark D. Tully, José-María Carazo, José L. Carrascosa, Pedro M. Matias, Tiago M. Bandeiras
Publicado en: Scientific Reports, Edición 8/1, 2018, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-018-31997-z

Molecular structure of FoxE, the putative iron oxidase of Rhodobacter ferrooxidans SW2

Autores: Luis Pereira, Ivo H. Saraiva, A. Sofia F. Oliveira, Cláudio M. Soares, Ricardo O. Louro, Carlos Frazão
Publicado en: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Edición 1858/10, 2017, Página(s) 847-853, ISSN 0005-2728
Editor: Elsevier BV
DOI: 10.1016/j.bbabio.2017.07.002

Hydrogen Sulfide Biochemistry and Interplay with Other Gaseous Mediators in Mammalian Physiology

Autores: Alessandro Giuffrè, João B. Vicente
Publicado en: Oxidative Medicine and Cellular Longevity, Edición 2018, 2018, Página(s) 1-31, ISSN 1942-0900
Editor: Landes Bioscience
DOI: 10.1155/2018/6290931

Screening Pyridine Derivatives against Human Hydrogen Sulfide-synthesizing Enzymes by Orthogonal Methods

Autores: Karim Zuhra, Pedro M. F. Sousa, Giulia Paulini, Ana Rita Lemos, Zenta Kalme, Imants Bisenieks, Egils Bisenieks, Brigita Vigante, Gunars Duburs, Tiago M. Bandeiras, Luciano Saso, Alessandro Giuffrè, João B. Vicente
Publicado en: Scientific Reports, Edición 9/1, 2019, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-018-36994-w

Spectroelectrochemical insights into structural and redox properties of immobilized endonuclease III and its catalytically inactive mutant

Autores: Elin Moe, Filipe Rollo, Célia M. Silveira, Murat Sezer, Peter Hildebrandt, Smilja Todorovic
Publicado en: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Edición 188, 2018, Página(s) 149-154, ISSN 1386-1425
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.saa.2017.06.050

N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells

Autores: Karim Zuhra, Catarina S. Tomé, Letizia Masi, Giorgio Giardina, Giulia Paulini, Francesca Malagrinò, Elena Forte, João B. Vicente, Alessandro Giuffrè
Publicado en: Cells, Edición 8/8, 2019, Página(s) 828, ISSN 2073-4409
Editor: MDPI
DOI: 10.3390/cells8080828

The key role of glutamate 172 in the mechanism of type II NADH:quinone oxidoreductase of Staphylococcus aureus

Autores: Filipe M. Sousa, Filipa V. Sena, Ana P. Batista, Diogo Athayde, José A. Brito, Margarida Archer, A. Sofia F. Oliveira, Cláudio M. Soares, Teresa Catarino, Manuela M. Pereira
Publicado en: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Edición 1858/10, 2017, Página(s) 823-832, ISSN 0005-2728
Editor: Elsevier BV
DOI: 10.1016/j.bbabio.2017.08.002

Analysis of a new flavodiiron core structural arrangement in Flv1-ΔFlR protein from Synechocystis sp. PCC6803

Autores: Patrícia T. Borges, Célia V. Romão, Lígia M. Saraiva, Vera L. Gonçalves, Maria A. Carrondo, Miguel Teixeira, Carlos Frazão
Publicado en: Journal of Structural Biology, Edición 205/1, 2019, Página(s) 91-102, ISSN 1047-8477
Editor: Academic Press
DOI: 10.1016/j.jsb.2018.11.004

Protein conformational flexibility modulates kinetics and thermodynamics of drug binding

Autores: M. Amaral, D. B. Kokh, J. Bomke, A. Wegener, H. P. Buchstaller, H. M. Eggenweiler, P. Matias, C. Sirrenberg, R. C. Wade, M. Frech
Publicado en: Nature Communications, Edición 8/1, 2017, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-017-02258-w

How superoxide reductases and flavodiiron proteins combat oxidative stress in anaerobes

Autores: Maria C. Martins, Célia V. Romão, Filipe Folgosa, Patrícia T. Borges, Carlos Frazão, Miguel Teixeira
Publicado en: Free Radical Biology and Medicine, Edición 140, 2019, Página(s) 36-60, ISSN 0891-5849
Editor: Elsevier BV
DOI: 10.1016/j.freeradbiomed.2019.01.051

Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility

Autores: Inês B. Trindade, José M. Silva, Bruno M. Fonseca, Teresa Catarino, Masaki Fujita, Pedro M. Matias, Elin Moe, Ricardo O. Louro
Publicado en: Journal of Biological Chemistry, Edición 294/1, 2019, Página(s) 157-167, ISSN 0021-9258
Editor: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra118.005041

A Clinically Relevant Variant of the Human Hydrogen Sulfide-Synthesizing Enzyme Cystathionine β -Synthase: Increased CO Reactivity as a Novel Molecular Mechanism of Pathogenicity?

Autores: João B. Vicente, Henrique G. Colaço, Francesca Malagrinò, Paulo E. Santo, André Gutierres, Tiago M. Bandeiras, Paula Leandro, José A. Brito, Alessandro Giuffrè
Publicado en: Oxidative Medicine and Cellular Longevity, Edición 2017, 2017, Página(s) 1-13, ISSN 1942-0900
Editor: Landes Bioscience
DOI: 10.1155/2017/8940321

Fluorinated ionic liquids for protein drug delivery systems: Investigating their impact on the structure and function of lysozyme

Autores: Márcia Alves, Nicole S.M. Vieira, Luís Paulo N. Rebelo, João M.M. Araújo, Ana B. Pereiro, Margarida Archer
Publicado en: International Journal of Pharmaceutics, Edición 526/1-2, 2017, Página(s) 309-320, ISSN 0378-5173
Editor: Elsevier BV
DOI: 10.1016/j.ijpharm.2017.05.002

The three Endonuclease III variants of Deinococcus radiodurans possess distinct and complementary DNA repair activities

Autores: Aili Sarre, Meike Stelter, Filipe Rollo, Salvatore De Bonis, Anna Seck, Cécilia Hognon, Jean-Luc Ravanat, Antonio Monari, François Dehez, Elin Moe, Joanna Timmins
Publicado en: DNA Repair, Edición 78, 2019, Página(s) 45-59, ISSN 1568-7864
Editor: Elsevier BV
DOI: 10.1016/j.dnarep.2019.03.014

A putative siderophore-interacting protein from the marine bacterium Shewanella frigidimarina NCIMB 400: cloning, expression, purification, crystallization and X-ray diffraction analysis

Autores: Inês B. Trindade, Bruno M. Fonseca, Pedro M. Matias, Ricardo O. Louro, Elin Moe
Publicado en: Acta Crystallographica Section F Structural Biology Communications, Edición 72/9, 2016, Página(s) 667-671, ISSN 2053-230X
Editor: John Wiley and Sons Ltd
DOI: 10.1107/s2053230x16011419

A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase

Autores: Sónia Zacarias, Adriana Temporão, Melisa del Barrio, Vincent Fourmond, Christophe Léger, Pedro M. Matias, Inês A. C. Pereira
Publicado en: ACS Catalysis, Edición 9/9, 2019, Página(s) 8509-8519, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.9b02347

Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase

Autores: Célia V. Romão, João B. Vicente, Patrícia T. Borges, Bruno L. Victor, Pedro Lamosa, Elísio Silva, Luís Pereira, Tiago M. Bandeiras, Cláudio M. Soares, Maria A. Carrondo, David Turner, Miguel Teixeira, Carlos Frazão
Publicado en: Journal of Molecular Biology, Edición 428/23, 2016, Página(s) 4686-4707, ISSN 0022-2836
Editor: Academic Press
DOI: 10.1016/j.jmb.2016.10.008

PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination

Autores: Thomas L. Clarke, Maria Pilar Sanchez-Bailon, Kelly Chiang, John J. Reynolds, Joaquin Herrero-Ruiz, Tiago M. Bandeiras, Pedro M. Matias, Sarah L. Maslen, J. Mark Skehel, Grant S. Stewart, Clare C. Davies
Publicado en: Molecular Cell, Edición 65/5, 2017, Página(s) 900-916.e7, ISSN 1097-2765
Editor: Cell Press
DOI: 10.1016/j.molcel.2017.01.019

Bioenergetic relevance of hydrogen sulfide and the interplay between gasotransmitters at human cystathionine β-synthase

Autores: João B. Vicente, Francesca Malagrinò, Marzia Arese, Elena Forte, Paolo Sarti, Alessandro Giuffrè
Publicado en: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Edición 1857/8, 2016, Página(s) 1127-1138, ISSN 0005-2728
Editor: Elsevier BV
DOI: 10.1016/j.bbabio.2016.03.030

The direct role of selenocysteine in [NiFeSe] hydrogenase maturation and catalysis

Autores: Marta C Marques, Cristina Tapia, Oscar Gutiérrez-Sanz, Ana Raquel Ramos, Kimberly L Keller, Judy D Wall, Antonio L De Lacey, Pedro M Matias, Inês A C Pereira
Publicado en: Nature Chemical Biology, Edición 13/5, 2017, Página(s) 544-550, ISSN 1552-4450
Editor: Nature Publishing Group
DOI: 10.1038/nchembio.2335

The rice cold-responsive calcium-dependent protein kinase OsCPK17 is regulated by alternative splicing and post-translational modifications

Autores: M. Cecília Almadanim, Nuno M. Gonçalves, Margarida T.G. Rosa, Bruno M. Alexandre, André M. Cordeiro, Mafalda Rodrigues, Nelson J.M. Saibo, Cláudio M. Soares, Célia V. Romão, M. Margarida Oliveira, Isabel A. Abreu
Publicado en: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Edición 1865/2, 2018, Página(s) 231-246, ISSN 0167-4889
Editor: Elsevier BV
DOI: 10.1016/j.bbamcr.2017.10.010

Desulfovibrio vulgaris CbiK P cobaltochelatase: evolution of a haem binding protein orchestrated by the incorporation of two histidine residues

Autores: Susana A.L. Lobo, Marco A.M. Videira, Isabel Pacheco, Mark N. Wass, Martin J. Warren, Miguel Teixeira, Pedro M. Matias, Célia V. Romão, Lígia M. Saraiva
Publicado en: Environmental Microbiology, Edición 19/1, 2016, Página(s) 106-118, ISSN 1462-2912
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.13479

Electron Accepting Units of the Diheme Cytochrome c TsdA, a Bifunctional Thiosulfate Dehydrogenase/Tetrathionate Reductase

Autores: Julia M. Kurth, José A. Brito, Jula Reuter, Alexander Flegler, Tobias Koch, Thomas Franke, Eva-Maria Klein, Sam F. Rowe, Julea N. Butt, Kevin Denkmann, Inês A. C. Pereira, Margarida Archer, Christiane Dahl
Publicado en: Journal of Biological Chemistry, Edición 291/48, 2016, Página(s) 24804-24818, ISSN 0021-9258
Editor: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.m116.753863

Optimizing Electroactive Organisms: The Effect of Orthologous Proteins

Autores: Bruno M. Fonseca, Luís Silva, Inês B. Trindade, Elin Moe, Pedro M. Matias, Ricardo O. Louro, Catarina M. Paquete
Publicado en: Frontiers in Energy Research, Edición 7, 2019, ISSN 2296-598X
Editor: Frontiers in Energy Research
DOI: 10.3389/fenrg.2019.00002

A Decade of Biochemical and Structural Studies of the DNA Repair Machinery of Deinococcus radiodurans: Major Findings, Functional and Mechanistic Insight and Challenges

Autores: Joanna Timmins, Elin Moe
Publicado en: Computational and Structural Biotechnology Journal, Edición 14, 2016, Página(s) 168-176, ISSN 2001-0370
Editor: ELSEVIER
DOI: 10.1016/j.csbj.2016.04.001

Clickable 4-Oxo-β-lactam-Based Selective Probing for Human Neutrophil Elastase Related Proteomes

Autores: Eduardo F. P. Ruivo, Lídia M. Gonçalves, Luís A. R. Carvalho, Rita C. Guedes, Stefan Hofbauer, José A. Brito, Margarida Archer, Rui Moreira, Susana D. Lucas
Publicado en: ChemMedChem, Edición 11/18, 2016, Página(s) 2037-2042, ISSN 1860-7179
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.201600258

A novel bacterial class V dye-decolourizing peroxidase from the extremophile Deinococcus radiodurans : cloning, expression optimization, purification, crystallization, initial characterization and X-ray diffraction analysis

Autores: Kelly Stefany Tuna Frade, Andreia Cecília Pimenta Fernandes, Celia Marisa Silveira, Carlos Frazão, Elin Moe
Publicado en: Acta Crystallographica Section F Structural Biology Communications, Edición 74/7, 2018, Página(s) 419-424, ISSN 2053-230X
Editor: John Wiley and Sons Ltd
DOI: 10.1107/s2053230x18008488

Insights into the Structures of Superoxide Reductases from the Symbionts Ignicoccus hospitalis and Nanoarchaeum equitans

Autores: Célia V. Romão, Pedro M. Matias, Cristiana M. Sousa, Filipa G. Pinho, Ana F. Pinto, Miguel Teixeira, Tiago M. Bandeiras
Publicado en: Biochemistry, Edición 57/36, 2018, Página(s) 5271-5281, ISSN 0006-2960
Editor: American Chemical Society
DOI: 10.1021/acs.biochem.8b00334

Continuous flexibility analysis of SARS-CoV-2 spike prefusion structures

Autores: R. Melero, C.O.S. Sorzano, B. Foster, J.L. Vilas, M. Martínez, R. Marabini, E. Ramírez-Aportela, R. Sánchez-García, D. Herreros, L. del Caño, P. Losana, Y. Fonseca-Reyna, P. Conesa, D. Wrapp, P. Chacón, J.S. McLellan, H.D. Tagare, J.M. Carazo
Publicado en: IUCrJ, Edición 7, 2020, Página(s) 1059-1069, ISSN 2052-2525
Editor: International Union of Crystallography
DOI: 10.1107/s2052252520012725

Advances in Xmipp for Cryo-electron Microscopy: From Xmipp to Scipion

Autores: D. Strelak, A. Jiménez-Moreno, J.L. Vilas, E. Ramírez-Aportela, R. Sánchez-García, D. Maluenda, J. Vargas, D. Herreros, E. Fernández-Giménez, F. de Isidro-Gómez, J. Horacek, D. Myska, M. Horacek, P. Conesa, Y.C. Fonseca-Reyna, J. Jiménez, M. Martínez, M. Harastani, S. Jonic, J. Filipovic, R. Marabini, J.M. Carazo, C.O.S. Sorzano.
Publicado en: Molecules, Edición 26 / 20, 2021, Página(s) 6224, ISSN 1420-3049
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26206224

Image processing tools for the validation of CryoEM maps

Autores: C.O.S. Sorzano, J.L. Vilas, E. Ramírez-Aportela, D. del Hoyo, D. Herreros, E. Fernandez-Giménez, D. Marchán, F. de Isidro Gómez, J.R. Macías, I. Sánchez, L. del Caño, Y. Fonseca-Reyna, P. Conesa, A. García-Mena, J. Burguet, J. García Condado, J. Méndez García, M. Martínez, A. Muñoz Barrutia, R. Marabini, J. Vargas, J.M. Carazo.
Publicado en: Faraday Discussions, Edición 240, 2022, Página(s) 210-227, ISSN 1359-6640
Editor: Royal Society of Chemistry
DOI: 10.1039/d2fd00059h

Book chapters (1)

Cytochrome bd and Gaseous Ligands in Bacterial Physiology

Autores: Elena Forte, Vitaliy B. Borisov, João B. Vicente, Alessandro Giuffrè
Publicado en: Advances in Microbial Physiology volume 71, Edición 71, 2017, Página(s) 171-234, ISBN 9780-128123850
Editor: Elsevier
DOI: 10.1016/bs.ampbs.2017.05.002