Imaging life from Molecules to cells - building knowledge on Cryo-electron microscopy methodologies | IMpaCT | Project | Results | H2020 | CORDIS

Risultati finali

D4.5 Report on Dissemination, Communication & Exploitation activities

Different activities are planned for dissemination Thus a report will be prepared by the end of the project reporting all the different activities performed during the project

D2.6 Part II - Scientific seminars from international researchers on Cryo-EM areas

A total of 2 seminars per year will be organized at ITQB NOVA to be presented by scientists well established in the field The selection of speakers will consider the different methodologies of the CryoEM technology and as much as possible cover research scientific projects overlapping those of the MX Unit One of the goals will be to bring scientists recognized as landmarks in the CryoEM methodologies for instance those that were awarded the 2017 Nobel Prize of Chemistry This deliverable concerns to the second part of these seminars

D2.3 Part I - Scientific seminars from international researchers on Cryo-EM areas

D 4.3 Reports on the Scientific conferences participation

Dissemination will be achieved mainly through participation in scientific conferences and thematic workshops and publishing of the results in high impact specialized peerreviewed journals We will aim to participate in scientific conferences specific to the different research projects that are ongoing in our MX Unit namely cancer viral infections and cellular substructures results of the different projects with major contributions directly arising from the shortterm visits to the Widening partners will be especially highlighted

D3.2 Plan of the career development short courses for early-stage researchers

A devoted WP (WP3) to the career development of early-stage researchers are planned within the proposal. The activities planned are training session regarding grant and scientific writing; communicating science, and IPR training. Thus a detail plan of the career development short courses for early-stage researchers will be performed based on the discussions that will occur during the kick-off-meeting.

D4.4 Participant evaluation of the Mini-symposium Pharmaceutical Cryo-EM applications

Our exploitation strategy will include the organization of a minisymposium targeted at pharmaceutical companies to raise awareness to the potential contribution of CryoEM to drug discovery and development pipelines One of the goals will be to collect the views and ideas of the industrial participants about their needs and problems to which science may provide solutions Different pharmaceutical companies will be invited to participate those that have already experience in CryoEM methodologies and others that have not started their studies using CryoEM The impact will be evaluated based on the quiz to the participants and on the diversity of the different pharmaceutical companies that will participate in the minisymposium

D 4.2 Outreach activities

Outreach activities will be promoted for the general public but also devoted to students from high school undergraduate master and doctoral levels The goal is to communicate the vast implications of Structural Biology by setting the basis for a twoway dialogue with target audiences increase the visibility and reputation of the project and partners perhaps also encouraging some of the young people to consider careers in science

Pubblicazioni

Improvements on marker-free images alignment for electron tomography

Autori: C.O.S. Sorzano, F. de Isidro-Gómez, E. Fernández-Giménez, D. Herreros, S. Marco, J.M. Carazo, C. Messaoudi.
Pubblicato in: Journal of Structural Biology X, Numero 4, 2020, Pagina/e 137, ISSN 2590-1524
Editore: Elsevier
DOI: 10.1016/j.yjsbx.2020.100037

The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stress

Autori: Sandra P. Santos, Yang Yang, Margarida T. G. Rosa, Mafalda A. A. Rodrigues, Claire Bouthier De La Tour, Suzanne Sommer, Miguel Teixeira, Maria A. Carrondo, Peter Cloetens, Isabel A. Abreu, Célia V. Romão
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-019-53140-2

Studies of multifunctional DNA polymerase I from the extremely radiation resistant Deinococcus radiodurans: recombinant expression, purification and characterization of the full-length protein and its large fragment

Autori: Fernandes, A., Piotrowski, Y., Williamson, A. Frade, K, and Moe, E.
Pubblicato in: Protein Expression and Purification, 2021, Pagina/e 105925, ISSN 1046-5928
Editore: Academic Press
DOI: 10.1016/j.pep.2021.105925

In vitro neutralisation of Zika virus by an engineered protein targeting the viral envelope fusion loop

Autori: Isabelle F. T. Viana, Carlos H. B. Cruz, Diogo Athayde, W. Camilla S. Adan, Lícya S. S. Xavier, Margarida Archer and Roberto D. Lins
Pubblicato in: Molecular Systems Design & Engineering, Numero 8, 2023, Pagina/e 516-526, ISSN 2058-9689
Editore: Royal Society of Chemistry
DOI: 10.1039/d2me00170e

Algorithmic robustness to preferred orientations in Single Particle Analysis by CryoEM

Autori: C.O.S. Sorzano, D. Semchonok, S.C. Lin, J.L. Vilas, A. Jimenez-Moreno, M. Gragera, D. Maluenda, M. Martinez, E. Ramirez-Aportela, R. Melero, A. Cuervo, P. Conesa, L. del Caño, Y.C. Fonseca, R. Sánchez-García, D. Strelak, E. Fernández-Giménez, F. de Isidro, P. Kastritis, R. Marabini, B. Bruce, J.M. Carazo.
Pubblicato in: Journal of Structural Biology, Numero 213 / 1, 2021, Pagina/e 107695, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2020.107695

On bias, variance, overfitting, gold standard and consensus in single-particle analysis by cryo-electron microscopy

Autori: C.O.S. Sorzano, A. Jimenez-Moreno, D. Maluenda, M. Martinez, E. Ramirez-Aportela, R. Melero, A. Cuervo, J. Conesa, J. Filipovic, P.Conesa, L. del Caño, Y.C. Fonseca, J. Jiménez-de la Morena, P. Losana, R. Sáchez-García, D. Strelak, E. Fernandez-Gimenez, F. de Isidro, D.Herreros, J.L. Vilas, R. Marabini, J.M. Carazo
Pubblicato in: Acta Crystallographica Section D, Numero 78 / 4, 2022, Pagina/e 410-423, ISSN 2059-7983
Editore: International Union of Crystallography
DOI: 10.1107/s2059798322001978

Identification of a conserved virion-stabilizing network inside the interprotomer pocket of enteroviruses

Autori: Flatt, J.W., Domanska, A., Seppälä, A.L., Butcher, S.J.
Pubblicato in: Communications Biology, Numero 4, 2021, Pagina/e 250, ISSN 2399-3642
Editore: SpringerNature
DOI: 10.1038/s42003-021-01779-x

SARS-CoV-2 production, purification methods and UV inactivation for proteomics and structural studies

Autori: Plavec, Z., Domanska, A., Liu, X., Laine, P., Paulin, L., Varjosalo, M., Auvinen, P., Wolf, S.G., Anastasina, M., Butcher, S.J.
Pubblicato in: Viruses, Numero 14 / 9, 2022, Pagina/e 1989, ISSN 1999-4915
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14091989

Sweet and sour: an update on classic galactosemia

Autori: Ana I. Coelho, M. Estela Rubio-Gozalbo, João B. Vicente, Isabel Rivera
Pubblicato in: Journal of Inherited Metabolic Disease, Numero 40/3, 2017, Pagina/e 325-342, ISSN 0141-8955
Editore: Kluwer Academic Publishers
DOI: 10.1007/s10545-017-0029-3

Syntheses of the plant auxin conjugate 2- O -(indole-3-acetyl)- myo -inositol IAInos

Autori: Saúl Silva, Osvaldo S. Ascenso, Eva C. Lourenço, Margarida Archer, Christopher D. Maycock, M. Rita Ventura
Pubblicato in: Organic & Biomolecular Chemistry, Numero 16/38, 2018, Pagina/e 6860-6864, ISSN 1477-0520
Editore: Royal Society of Chemistry
DOI: 10.1039/c8ob02096e

SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions

Autori: Sandra P. Santos, Maxime G. Cuypers, Adam Round, Stephanie Finet, Theyencheri Narayanan, Edward P. Mitchell, Célia V. Romão
Pubblicato in: Journal of Molecular Biology, Numero 429/5, 2017, Pagina/e 667-687, ISSN 0022-2836
Editore: Academic Press
DOI: 10.1016/j.jmb.2017.01.008

Hydrogen Sulfide Oxidation: Adaptive Changes in Mitochondria of SW480 Colorectal Cancer Cells upon Exposure to Hypoxia

Autori: Francesca Malagrinò, Karim Zuhra, Ludovica Mascolo, Daniela Mastronicola, João B. Vicente, Elena Forte, Alessandro Giuffrè
Pubblicato in: Oxidative Medicine and Cellular Longevity, Numero 2019, 2019, Pagina/e 1-11, ISSN 1942-0900
Editore: Landes Bioscience
DOI: 10.1155/2019/8102936

X-ray structure of full-length human RuvB-Like 2 – mechanistic insights into coupling between ATP binding and mechanical action

Autori: Sara T. N. Silva, José A. Brito, Rocío Arranz, Carlos Óscar S. Sorzano, Christine Ebel, James Doutch, Mark D. Tully, José-María Carazo, José L. Carrascosa, Pedro M. Matias, Tiago M. Bandeiras
Pubblicato in: Scientific Reports, Numero 8/1, 2018, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-31997-z

Molecular structure of FoxE, the putative iron oxidase of Rhodobacter ferrooxidans SW2

Autori: Luis Pereira, Ivo H. Saraiva, A. Sofia F. Oliveira, Cláudio M. Soares, Ricardo O. Louro, Carlos Frazão
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Numero 1858/10, 2017, Pagina/e 847-853, ISSN 0005-2728
Editore: Elsevier BV
DOI: 10.1016/j.bbabio.2017.07.002

Hydrogen Sulfide Biochemistry and Interplay with Other Gaseous Mediators in Mammalian Physiology

Autori: Alessandro Giuffrè, João B. Vicente
Pubblicato in: Oxidative Medicine and Cellular Longevity, Numero 2018, 2018, Pagina/e 1-31, ISSN 1942-0900
Editore: Landes Bioscience
DOI: 10.1155/2018/6290931

Screening Pyridine Derivatives against Human Hydrogen Sulfide-synthesizing Enzymes by Orthogonal Methods

Autori: Karim Zuhra, Pedro M. F. Sousa, Giulia Paulini, Ana Rita Lemos, Zenta Kalme, Imants Bisenieks, Egils Bisenieks, Brigita Vigante, Gunars Duburs, Tiago M. Bandeiras, Luciano Saso, Alessandro Giuffrè, João B. Vicente
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-36994-w

Spectroelectrochemical insights into structural and redox properties of immobilized endonuclease III and its catalytically inactive mutant

Autori: Elin Moe, Filipe Rollo, Célia M. Silveira, Murat Sezer, Peter Hildebrandt, Smilja Todorovic
Pubblicato in: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Numero 188, 2018, Pagina/e 149-154, ISSN 1386-1425
Editore: Pergamon Press Ltd.
DOI: 10.1016/j.saa.2017.06.050

N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells

Autori: Karim Zuhra, Catarina S. Tomé, Letizia Masi, Giorgio Giardina, Giulia Paulini, Francesca Malagrinò, Elena Forte, João B. Vicente, Alessandro Giuffrè
Pubblicato in: Cells, Numero 8/8, 2019, Pagina/e 828, ISSN 2073-4409
Editore: MDPI
DOI: 10.3390/cells8080828

The key role of glutamate 172 in the mechanism of type II NADH:quinone oxidoreductase of Staphylococcus aureus

Autori: Filipe M. Sousa, Filipa V. Sena, Ana P. Batista, Diogo Athayde, José A. Brito, Margarida Archer, A. Sofia F. Oliveira, Cláudio M. Soares, Teresa Catarino, Manuela M. Pereira
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Numero 1858/10, 2017, Pagina/e 823-832, ISSN 0005-2728
Editore: Elsevier BV
DOI: 10.1016/j.bbabio.2017.08.002

Analysis of a new flavodiiron core structural arrangement in Flv1-ΔFlR protein from Synechocystis sp. PCC6803

Autori: Patrícia T. Borges, Célia V. Romão, Lígia M. Saraiva, Vera L. Gonçalves, Maria A. Carrondo, Miguel Teixeira, Carlos Frazão
Pubblicato in: Journal of Structural Biology, Numero 205/1, 2019, Pagina/e 91-102, ISSN 1047-8477
Editore: Academic Press
DOI: 10.1016/j.jsb.2018.11.004

Protein conformational flexibility modulates kinetics and thermodynamics of drug binding

Autori: M. Amaral, D. B. Kokh, J. Bomke, A. Wegener, H. P. Buchstaller, H. M. Eggenweiler, P. Matias, C. Sirrenberg, R. C. Wade, M. Frech
Pubblicato in: Nature Communications, Numero 8/1, 2017, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-017-02258-w

How superoxide reductases and flavodiiron proteins combat oxidative stress in anaerobes

Autori: Maria C. Martins, Célia V. Romão, Filipe Folgosa, Patrícia T. Borges, Carlos Frazão, Miguel Teixeira
Pubblicato in: Free Radical Biology and Medicine, Numero 140, 2019, Pagina/e 36-60, ISSN 0891-5849
Editore: Elsevier BV
DOI: 10.1016/j.freeradbiomed.2019.01.051

Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility

Autori: Inês B. Trindade, José M. Silva, Bruno M. Fonseca, Teresa Catarino, Masaki Fujita, Pedro M. Matias, Elin Moe, Ricardo O. Louro
Pubblicato in: Journal of Biological Chemistry, Numero 294/1, 2019, Pagina/e 157-167, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra118.005041

A Clinically Relevant Variant of the Human Hydrogen Sulfide-Synthesizing Enzyme Cystathionine β -Synthase: Increased CO Reactivity as a Novel Molecular Mechanism of Pathogenicity?

Autori: João B. Vicente, Henrique G. Colaço, Francesca Malagrinò, Paulo E. Santo, André Gutierres, Tiago M. Bandeiras, Paula Leandro, José A. Brito, Alessandro Giuffrè
Pubblicato in: Oxidative Medicine and Cellular Longevity, Numero 2017, 2017, Pagina/e 1-13, ISSN 1942-0900
Editore: Landes Bioscience
DOI: 10.1155/2017/8940321

Fluorinated ionic liquids for protein drug delivery systems: Investigating their impact on the structure and function of lysozyme

Autori: Márcia Alves, Nicole S.M. Vieira, Luís Paulo N. Rebelo, João M.M. Araújo, Ana B. Pereiro, Margarida Archer
Pubblicato in: International Journal of Pharmaceutics, Numero 526/1-2, 2017, Pagina/e 309-320, ISSN 0378-5173
Editore: Elsevier BV
DOI: 10.1016/j.ijpharm.2017.05.002

The three Endonuclease III variants of Deinococcus radiodurans possess distinct and complementary DNA repair activities

Autori: Aili Sarre, Meike Stelter, Filipe Rollo, Salvatore De Bonis, Anna Seck, Cécilia Hognon, Jean-Luc Ravanat, Antonio Monari, François Dehez, Elin Moe, Joanna Timmins
Pubblicato in: DNA Repair, Numero 78, 2019, Pagina/e 45-59, ISSN 1568-7864
Editore: Elsevier BV
DOI: 10.1016/j.dnarep.2019.03.014

A putative siderophore-interacting protein from the marine bacterium Shewanella frigidimarina NCIMB 400: cloning, expression, purification, crystallization and X-ray diffraction analysis

Autori: Inês B. Trindade, Bruno M. Fonseca, Pedro M. Matias, Ricardo O. Louro, Elin Moe
Pubblicato in: Acta Crystallographica Section F Structural Biology Communications, Numero 72/9, 2016, Pagina/e 667-671, ISSN 2053-230X
Editore: John Wiley and Sons Ltd
DOI: 10.1107/s2053230x16011419

A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase

Autori: Sónia Zacarias, Adriana Temporão, Melisa del Barrio, Vincent Fourmond, Christophe Léger, Pedro M. Matias, Inês A. C. Pereira
Pubblicato in: ACS Catalysis, Numero 9/9, 2019, Pagina/e 8509-8519, ISSN 2155-5435
Editore: American Chemical Society
DOI: 10.1021/acscatal.9b02347

Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase

Autori: Célia V. Romão, João B. Vicente, Patrícia T. Borges, Bruno L. Victor, Pedro Lamosa, Elísio Silva, Luís Pereira, Tiago M. Bandeiras, Cláudio M. Soares, Maria A. Carrondo, David Turner, Miguel Teixeira, Carlos Frazão
Pubblicato in: Journal of Molecular Biology, Numero 428/23, 2016, Pagina/e 4686-4707, ISSN 0022-2836
Editore: Academic Press
DOI: 10.1016/j.jmb.2016.10.008

PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination

Autori: Thomas L. Clarke, Maria Pilar Sanchez-Bailon, Kelly Chiang, John J. Reynolds, Joaquin Herrero-Ruiz, Tiago M. Bandeiras, Pedro M. Matias, Sarah L. Maslen, J. Mark Skehel, Grant S. Stewart, Clare C. Davies
Pubblicato in: Molecular Cell, Numero 65/5, 2017, Pagina/e 900-916.e7, ISSN 1097-2765
Editore: Cell Press
DOI: 10.1016/j.molcel.2017.01.019

Bioenergetic relevance of hydrogen sulfide and the interplay between gasotransmitters at human cystathionine β-synthase

Autori: João B. Vicente, Francesca Malagrinò, Marzia Arese, Elena Forte, Paolo Sarti, Alessandro Giuffrè
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Numero 1857/8, 2016, Pagina/e 1127-1138, ISSN 0005-2728
Editore: Elsevier BV
DOI: 10.1016/j.bbabio.2016.03.030

The direct role of selenocysteine in [NiFeSe] hydrogenase maturation and catalysis

Autori: Marta C Marques, Cristina Tapia, Oscar Gutiérrez-Sanz, Ana Raquel Ramos, Kimberly L Keller, Judy D Wall, Antonio L De Lacey, Pedro M Matias, Inês A C Pereira
Pubblicato in: Nature Chemical Biology, Numero 13/5, 2017, Pagina/e 544-550, ISSN 1552-4450
Editore: Nature Publishing Group
DOI: 10.1038/nchembio.2335

The rice cold-responsive calcium-dependent protein kinase OsCPK17 is regulated by alternative splicing and post-translational modifications

Autori: M. Cecília Almadanim, Nuno M. Gonçalves, Margarida T.G. Rosa, Bruno M. Alexandre, André M. Cordeiro, Mafalda Rodrigues, Nelson J.M. Saibo, Cláudio M. Soares, Célia V. Romão, M. Margarida Oliveira, Isabel A. Abreu
Pubblicato in: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Numero 1865/2, 2018, Pagina/e 231-246, ISSN 0167-4889
Editore: Elsevier BV
DOI: 10.1016/j.bbamcr.2017.10.010

Desulfovibrio vulgaris CbiK P cobaltochelatase: evolution of a haem binding protein orchestrated by the incorporation of two histidine residues

Autori: Susana A.L. Lobo, Marco A.M. Videira, Isabel Pacheco, Mark N. Wass, Martin J. Warren, Miguel Teixeira, Pedro M. Matias, Célia V. Romão, Lígia M. Saraiva
Pubblicato in: Environmental Microbiology, Numero 19/1, 2016, Pagina/e 106-118, ISSN 1462-2912
Editore: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.13479

Electron Accepting Units of the Diheme Cytochrome c TsdA, a Bifunctional Thiosulfate Dehydrogenase/Tetrathionate Reductase

Autori: Julia M. Kurth, José A. Brito, Jula Reuter, Alexander Flegler, Tobias Koch, Thomas Franke, Eva-Maria Klein, Sam F. Rowe, Julea N. Butt, Kevin Denkmann, Inês A. C. Pereira, Margarida Archer, Christiane Dahl
Pubblicato in: Journal of Biological Chemistry, Numero 291/48, 2016, Pagina/e 24804-24818, ISSN 0021-9258
Editore: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.m116.753863

Optimizing Electroactive Organisms: The Effect of Orthologous Proteins

Autori: Bruno M. Fonseca, Luís Silva, Inês B. Trindade, Elin Moe, Pedro M. Matias, Ricardo O. Louro, Catarina M. Paquete
Pubblicato in: Frontiers in Energy Research, Numero 7, 2019, ISSN 2296-598X
Editore: Frontiers in Energy Research
DOI: 10.3389/fenrg.2019.00002

A Decade of Biochemical and Structural Studies of the DNA Repair Machinery of Deinococcus radiodurans: Major Findings, Functional and Mechanistic Insight and Challenges

Autori: Joanna Timmins, Elin Moe
Pubblicato in: Computational and Structural Biotechnology Journal, Numero 14, 2016, Pagina/e 168-176, ISSN 2001-0370
Editore: ELSEVIER
DOI: 10.1016/j.csbj.2016.04.001

Clickable 4-Oxo-β-lactam-Based Selective Probing for Human Neutrophil Elastase Related Proteomes

Autori: Eduardo F. P. Ruivo, Lídia M. Gonçalves, Luís A. R. Carvalho, Rita C. Guedes, Stefan Hofbauer, José A. Brito, Margarida Archer, Rui Moreira, Susana D. Lucas
Pubblicato in: ChemMedChem, Numero 11/18, 2016, Pagina/e 2037-2042, ISSN 1860-7179
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.201600258

A novel bacterial class V dye-decolourizing peroxidase from the extremophile Deinococcus radiodurans : cloning, expression optimization, purification, crystallization, initial characterization and X-ray diffraction analysis

Autori: Kelly Stefany Tuna Frade, Andreia Cecília Pimenta Fernandes, Celia Marisa Silveira, Carlos Frazão, Elin Moe
Pubblicato in: Acta Crystallographica Section F Structural Biology Communications, Numero 74/7, 2018, Pagina/e 419-424, ISSN 2053-230X
Editore: John Wiley and Sons Ltd
DOI: 10.1107/s2053230x18008488

Insights into the Structures of Superoxide Reductases from the Symbionts Ignicoccus hospitalis and Nanoarchaeum equitans

Autori: Célia V. Romão, Pedro M. Matias, Cristiana M. Sousa, Filipa G. Pinho, Ana F. Pinto, Miguel Teixeira, Tiago M. Bandeiras
Pubblicato in: Biochemistry, Numero 57/36, 2018, Pagina/e 5271-5281, ISSN 0006-2960
Editore: American Chemical Society
DOI: 10.1021/acs.biochem.8b00334

Continuous flexibility analysis of SARS-CoV-2 spike prefusion structures

Autori: R. Melero, C.O.S. Sorzano, B. Foster, J.L. Vilas, M. Martínez, R. Marabini, E. Ramírez-Aportela, R. Sánchez-García, D. Herreros, L. del Caño, P. Losana, Y. Fonseca-Reyna, P. Conesa, D. Wrapp, P. Chacón, J.S. McLellan, H.D. Tagare, J.M. Carazo
Pubblicato in: IUCrJ, Numero 7, 2020, Pagina/e 1059-1069, ISSN 2052-2525
Editore: International Union of Crystallography
DOI: 10.1107/s2052252520012725

Advances in Xmipp for Cryo-electron Microscopy: From Xmipp to Scipion

Autori: D. Strelak, A. Jiménez-Moreno, J.L. Vilas, E. Ramírez-Aportela, R. Sánchez-García, D. Maluenda, J. Vargas, D. Herreros, E. Fernández-Giménez, F. de Isidro-Gómez, J. Horacek, D. Myska, M. Horacek, P. Conesa, Y.C. Fonseca-Reyna, J. Jiménez, M. Martínez, M. Harastani, S. Jonic, J. Filipovic, R. Marabini, J.M. Carazo, C.O.S. Sorzano.
Pubblicato in: Molecules, Numero 26 / 20, 2021, Pagina/e 6224, ISSN 1420-3049
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26206224

Image processing tools for the validation of CryoEM maps

Autori: C.O.S. Sorzano, J.L. Vilas, E. Ramírez-Aportela, D. del Hoyo, D. Herreros, E. Fernandez-Giménez, D. Marchán, F. de Isidro Gómez, J.R. Macías, I. Sánchez, L. del Caño, Y. Fonseca-Reyna, P. Conesa, A. García-Mena, J. Burguet, J. García Condado, J. Méndez García, M. Martínez, A. Muñoz Barrutia, R. Marabini, J. Vargas, J.M. Carazo.
Pubblicato in: Faraday Discussions, Numero 240, 2022, Pagina/e 210-227, ISSN 1359-6640
Editore: Royal Society of Chemistry
DOI: 10.1039/d2fd00059h

Cytochrome bd and Gaseous Ligands in Bacterial Physiology

Autori: Elena Forte, Vitaliy B. Borisov, João B. Vicente, Alessandro Giuffrè
Pubblicato in: Advances in Microbial Physiology volume 71, Numero 71, 2017, Pagina/e 171-234, ISBN 9780-128123850
Editore: Elsevier
DOI: 10.1016/bs.ampbs.2017.05.002

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Imaging life from Molecules to cells - building knowledge on Cryo-electron microscopy methodologies

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