Imaging life from Molecules to cells - building knowledge on Cryo-electron microscopy methodologies

Projektinformationen

IMpaCT

ID Finanzhilfevereinbarung: 857203

DOI

10.3030/857203

Projekt abgeschlossen

EK-Unterschriftsdatum 9 Juli 2019

Startdatum 1 September 2019

Enddatum 28 Februar 2023

Finanziert unter

• Twinning of research institutions

Gesamtkosten

€ 800 000,00

EU-Beitrag

€ 800 000,00

Koordiniert durch

UNIVERSIDADE NOVA DE LISBOA
Portugal

CORDIS bietet Links zu öffentlichen Ergebnissen und Veröffentlichungen von HORIZONT-Projekten.

Links zu Ergebnissen und Veröffentlichungen von RP7-Projekten sowie Links zu einigen Typen spezifischer Ergebnisse wie Datensätzen und Software werden dynamisch von OpenAIRE abgerufen.

Leistungen

Documents, reports (7)

D4.5 Report on Dissemination, Communication & Exploitation activities

Different activities are planned for dissemination Thus a report will be prepared by the end of the project reporting all the different activities performed during the project

D2.6 Part II - Scientific seminars from international researchers on Cryo-EM areas

A total of 2 seminars per year will be organized at ITQB NOVA to be presented by scientists well established in the field The selection of speakers will consider the different methodologies of the CryoEM technology and as much as possible cover research scientific projects overlapping those of the MX Unit One of the goals will be to bring scientists recognized as landmarks in the CryoEM methodologies for instance those that were awarded the 2017 Nobel Prize of Chemistry This deliverable concerns to the second part of these seminars

D2.3 Part I - Scientific seminars from international researchers on Cryo-EM areas

A total of 2 seminars per year will be organized at ITQB NOVA to be presented by scientists well established in the field The selection of speakers will consider the different methodologies of the CryoEM technology and as much as possible cover research scientific projects overlapping those of the MX Unit One of the goals will be to bring scientists recognized as landmarks in the CryoEM methodologies for instance those that were awarded the 2017 Nobel Prize of Chemistry This deliverable concerns to the first part of these seminars

D 4.3 Reports on the Scientific conferences participation

Dissemination will be achieved mainly through participation in scientific conferences and thematic workshops and publishing of the results in high impact specialized peerreviewed journals We will aim to participate in scientific conferences specific to the different research projects that are ongoing in our MX Unit namely cancer viral infections and cellular substructures results of the different projects with major contributions directly arising from the shortterm visits to the Widening partners will be especially highlighted

D3.2 Plan of the career development short courses for early-stage researchers

A devoted WP (WP3) to the career development of early-stage researchers are planned within the proposal. The activities planned are training session regarding grant and scientific writing; communicating science, and IPR training. Thus a detail plan of the career development short courses for early-stage researchers will be performed based on the discussions that will occur during the kick-off-meeting.

D4.4 Participant evaluation of the Mini-symposium Pharmaceutical Cryo-EM applications

Our exploitation strategy will include the organization of a minisymposium targeted at pharmaceutical companies to raise awareness to the potential contribution of CryoEM to drug discovery and development pipelines One of the goals will be to collect the views and ideas of the industrial participants about their needs and problems to which science may provide solutions Different pharmaceutical companies will be invited to participate those that have already experience in CryoEM methodologies and others that have not started their studies using CryoEM The impact will be evaluated based on the quiz to the participants and on the diversity of the different pharmaceutical companies that will participate in the minisymposium

D 4.2 Outreach activities

Outreach activities will be promoted for the general public but also devoted to students from high school undergraduate master and doctoral levels The goal is to communicate the vast implications of Structural Biology by setting the basis for a twoway dialogue with target audiences increase the visibility and reputation of the project and partners perhaps also encouraging some of the young people to consider careers in science

Veröffentlichungen

Peer reviewed articles (43)

Improvements on marker-free images alignment for electron tomography

Autoren: C.O.S. Sorzano, F. de Isidro-Gómez, E. Fernández-Giménez, D. Herreros, S. Marco, J.M. Carazo, C. Messaoudi.
Veröffentlicht in: Journal of Structural Biology X, Ausgabe 4, 2020, Seite(n) 137, ISSN 2590-1524
Herausgeber: Elsevier
DOI: 10.1016/j.yjsbx.2020.100037

The interplay between Mn and Fe in Deinococcus radiodurans triggers cellular protection during paraquat-induced oxidative stress

Autoren: Sandra P. Santos, Yang Yang, Margarida T. G. Rosa, Mafalda A. A. Rodrigues, Claire Bouthier De La Tour, Suzanne Sommer, Miguel Teixeira, Maria A. Carrondo, Peter Cloetens, Isabel A. Abreu, Célia V. Romão
Veröffentlicht in: Scientific Reports, Ausgabe 9/1, 2019, ISSN 2045-2322
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41598-019-53140-2

Studies of multifunctional DNA polymerase I from the extremely radiation resistant Deinococcus radiodurans: recombinant expression, purification and characterization of the full-length protein and its large fragment

Autoren: Fernandes, A., Piotrowski, Y., Williamson, A. Frade, K, and Moe, E.
Veröffentlicht in: Protein Expression and Purification, 2021, Seite(n) 105925, ISSN 1046-5928
Herausgeber: Academic Press
DOI: 10.1016/j.pep.2021.105925

In vitro neutralisation of Zika virus by an engineered protein targeting the viral envelope fusion loop

Autoren: Isabelle F. T. Viana, Carlos H. B. Cruz, Diogo Athayde, W. Camilla S. Adan, Lícya S. S. Xavier, Margarida Archer and Roberto D. Lins
Veröffentlicht in: Molecular Systems Design & Engineering, Ausgabe 8, 2023, Seite(n) 516-526, ISSN 2058-9689
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/d2me00170e

Algorithmic robustness to preferred orientations in Single Particle Analysis by CryoEM

Autoren: C.O.S. Sorzano, D. Semchonok, S.C. Lin, J.L. Vilas, A. Jimenez-Moreno, M. Gragera, D. Maluenda, M. Martinez, E. Ramirez-Aportela, R. Melero, A. Cuervo, P. Conesa, L. del Caño, Y.C. Fonseca, R. Sánchez-García, D. Strelak, E. Fernández-Giménez, F. de Isidro, P. Kastritis, R. Marabini, B. Bruce, J.M. Carazo.
Veröffentlicht in: Journal of Structural Biology, Ausgabe 213 / 1, 2021, Seite(n) 107695, ISSN 1047-8477
Herausgeber: Academic Press
DOI: 10.1016/j.jsb.2020.107695

On bias, variance, overfitting, gold standard and consensus in single-particle analysis by cryo-electron microscopy

Autoren: C.O.S. Sorzano, A. Jimenez-Moreno, D. Maluenda, M. Martinez, E. Ramirez-Aportela, R. Melero, A. Cuervo, J. Conesa, J. Filipovic, P.Conesa, L. del Caño, Y.C. Fonseca, J. Jiménez-de la Morena, P. Losana, R. Sáchez-García, D. Strelak, E. Fernandez-Gimenez, F. de Isidro, D.Herreros, J.L. Vilas, R. Marabini, J.M. Carazo
Veröffentlicht in: Acta Crystallographica Section D, Ausgabe 78 / 4, 2022, Seite(n) 410-423, ISSN 2059-7983
Herausgeber: International Union of Crystallography
DOI: 10.1107/s2059798322001978

Identification of a conserved virion-stabilizing network inside the interprotomer pocket of enteroviruses

Autoren: Flatt, J.W., Domanska, A., Seppälä, A.L., Butcher, S.J.
Veröffentlicht in: Communications Biology, Ausgabe 4, 2021, Seite(n) 250, ISSN 2399-3642
Herausgeber: SpringerNature
DOI: 10.1038/s42003-021-01779-x

SARS-CoV-2 production, purification methods and UV inactivation for proteomics and structural studies

Autoren: Plavec, Z., Domanska, A., Liu, X., Laine, P., Paulin, L., Varjosalo, M., Auvinen, P., Wolf, S.G., Anastasina, M., Butcher, S.J.
Veröffentlicht in: Viruses, Ausgabe 14 / 9, 2022, Seite(n) 1989, ISSN 1999-4915
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v14091989

Sweet and sour: an update on classic galactosemia

Autoren: Ana I. Coelho, M. Estela Rubio-Gozalbo, João B. Vicente, Isabel Rivera
Veröffentlicht in: Journal of Inherited Metabolic Disease, Ausgabe 40/3, 2017, Seite(n) 325-342, ISSN 0141-8955
Herausgeber: Kluwer Academic Publishers
DOI: 10.1007/s10545-017-0029-3

Syntheses of the plant auxin conjugate 2- O -(indole-3-acetyl)- myo -inositol IAInos

Autoren: Saúl Silva, Osvaldo S. Ascenso, Eva C. Lourenço, Margarida Archer, Christopher D. Maycock, M. Rita Ventura
Veröffentlicht in: Organic & Biomolecular Chemistry, Ausgabe 16/38, 2018, Seite(n) 6860-6864, ISSN 1477-0520
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/c8ob02096e

SAXS Structural Studies of Dps from Deinococcus radiodurans Highlights the Conformation of the Mobile N-Terminal Extensions

Autoren: Sandra P. Santos, Maxime G. Cuypers, Adam Round, Stephanie Finet, Theyencheri Narayanan, Edward P. Mitchell, Célia V. Romão
Veröffentlicht in: Journal of Molecular Biology, Ausgabe 429/5, 2017, Seite(n) 667-687, ISSN 0022-2836
Herausgeber: Academic Press
DOI: 10.1016/j.jmb.2017.01.008

Hydrogen Sulfide Oxidation: Adaptive Changes in Mitochondria of SW480 Colorectal Cancer Cells upon Exposure to Hypoxia

Autoren: Francesca Malagrinò, Karim Zuhra, Ludovica Mascolo, Daniela Mastronicola, João B. Vicente, Elena Forte, Alessandro Giuffrè
Veröffentlicht in: Oxidative Medicine and Cellular Longevity, Ausgabe 2019, 2019, Seite(n) 1-11, ISSN 1942-0900
Herausgeber: Landes Bioscience
DOI: 10.1155/2019/8102936

X-ray structure of full-length human RuvB-Like 2 – mechanistic insights into coupling between ATP binding and mechanical action

Autoren: Sara T. N. Silva, José A. Brito, Rocío Arranz, Carlos Óscar S. Sorzano, Christine Ebel, James Doutch, Mark D. Tully, José-María Carazo, José L. Carrascosa, Pedro M. Matias, Tiago M. Bandeiras
Veröffentlicht in: Scientific Reports, Ausgabe 8/1, 2018, ISSN 2045-2322
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41598-018-31997-z

Molecular structure of FoxE, the putative iron oxidase of Rhodobacter ferrooxidans SW2

Autoren: Luis Pereira, Ivo H. Saraiva, A. Sofia F. Oliveira, Cláudio M. Soares, Ricardo O. Louro, Carlos Frazão
Veröffentlicht in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Ausgabe 1858/10, 2017, Seite(n) 847-853, ISSN 0005-2728
Herausgeber: Elsevier BV
DOI: 10.1016/j.bbabio.2017.07.002

Hydrogen Sulfide Biochemistry and Interplay with Other Gaseous Mediators in Mammalian Physiology

Autoren: Alessandro Giuffrè, João B. Vicente
Veröffentlicht in: Oxidative Medicine and Cellular Longevity, Ausgabe 2018, 2018, Seite(n) 1-31, ISSN 1942-0900
Herausgeber: Landes Bioscience
DOI: 10.1155/2018/6290931

Screening Pyridine Derivatives against Human Hydrogen Sulfide-synthesizing Enzymes by Orthogonal Methods

Autoren: Karim Zuhra, Pedro M. F. Sousa, Giulia Paulini, Ana Rita Lemos, Zenta Kalme, Imants Bisenieks, Egils Bisenieks, Brigita Vigante, Gunars Duburs, Tiago M. Bandeiras, Luciano Saso, Alessandro Giuffrè, João B. Vicente
Veröffentlicht in: Scientific Reports, Ausgabe 9/1, 2019, ISSN 2045-2322
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41598-018-36994-w

Spectroelectrochemical insights into structural and redox properties of immobilized endonuclease III and its catalytically inactive mutant

Autoren: Elin Moe, Filipe Rollo, Célia M. Silveira, Murat Sezer, Peter Hildebrandt, Smilja Todorovic
Veröffentlicht in: Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Ausgabe 188, 2018, Seite(n) 149-154, ISSN 1386-1425
Herausgeber: Pergamon Press Ltd.
DOI: 10.1016/j.saa.2017.06.050

N-Acetylcysteine Serves as Substrate of 3-Mercaptopyruvate Sulfurtransferase and Stimulates Sulfide Metabolism in Colon Cancer Cells

Autoren: Karim Zuhra, Catarina S. Tomé, Letizia Masi, Giorgio Giardina, Giulia Paulini, Francesca Malagrinò, Elena Forte, João B. Vicente, Alessandro Giuffrè
Veröffentlicht in: Cells, Ausgabe 8/8, 2019, Seite(n) 828, ISSN 2073-4409
Herausgeber: MDPI
DOI: 10.3390/cells8080828

The key role of glutamate 172 in the mechanism of type II NADH:quinone oxidoreductase of Staphylococcus aureus

Autoren: Filipe M. Sousa, Filipa V. Sena, Ana P. Batista, Diogo Athayde, José A. Brito, Margarida Archer, A. Sofia F. Oliveira, Cláudio M. Soares, Teresa Catarino, Manuela M. Pereira
Veröffentlicht in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Ausgabe 1858/10, 2017, Seite(n) 823-832, ISSN 0005-2728
Herausgeber: Elsevier BV
DOI: 10.1016/j.bbabio.2017.08.002

Analysis of a new flavodiiron core structural arrangement in Flv1-ΔFlR protein from Synechocystis sp. PCC6803

Autoren: Patrícia T. Borges, Célia V. Romão, Lígia M. Saraiva, Vera L. Gonçalves, Maria A. Carrondo, Miguel Teixeira, Carlos Frazão
Veröffentlicht in: Journal of Structural Biology, Ausgabe 205/1, 2019, Seite(n) 91-102, ISSN 1047-8477
Herausgeber: Academic Press
DOI: 10.1016/j.jsb.2018.11.004

Protein conformational flexibility modulates kinetics and thermodynamics of drug binding

Autoren: M. Amaral, D. B. Kokh, J. Bomke, A. Wegener, H. P. Buchstaller, H. M. Eggenweiler, P. Matias, C. Sirrenberg, R. C. Wade, M. Frech
Veröffentlicht in: Nature Communications, Ausgabe 8/1, 2017, ISSN 2041-1723
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41467-017-02258-w

How superoxide reductases and flavodiiron proteins combat oxidative stress in anaerobes

Autoren: Maria C. Martins, Célia V. Romão, Filipe Folgosa, Patrícia T. Borges, Carlos Frazão, Miguel Teixeira
Veröffentlicht in: Free Radical Biology and Medicine, Ausgabe 140, 2019, Seite(n) 36-60, ISSN 0891-5849
Herausgeber: Elsevier BV
DOI: 10.1016/j.freeradbiomed.2019.01.051

Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility

Autoren: Inês B. Trindade, José M. Silva, Bruno M. Fonseca, Teresa Catarino, Masaki Fujita, Pedro M. Matias, Elin Moe, Ricardo O. Louro
Veröffentlicht in: Journal of Biological Chemistry, Ausgabe 294/1, 2019, Seite(n) 157-167, ISSN 0021-9258
Herausgeber: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra118.005041

A Clinically Relevant Variant of the Human Hydrogen Sulfide-Synthesizing Enzyme Cystathionine β -Synthase: Increased CO Reactivity as a Novel Molecular Mechanism of Pathogenicity?

Autoren: João B. Vicente, Henrique G. Colaço, Francesca Malagrinò, Paulo E. Santo, André Gutierres, Tiago M. Bandeiras, Paula Leandro, José A. Brito, Alessandro Giuffrè
Veröffentlicht in: Oxidative Medicine and Cellular Longevity, Ausgabe 2017, 2017, Seite(n) 1-13, ISSN 1942-0900
Herausgeber: Landes Bioscience
DOI: 10.1155/2017/8940321

Fluorinated ionic liquids for protein drug delivery systems: Investigating their impact on the structure and function of lysozyme

Autoren: Márcia Alves, Nicole S.M. Vieira, Luís Paulo N. Rebelo, João M.M. Araújo, Ana B. Pereiro, Margarida Archer
Veröffentlicht in: International Journal of Pharmaceutics, Ausgabe 526/1-2, 2017, Seite(n) 309-320, ISSN 0378-5173
Herausgeber: Elsevier BV
DOI: 10.1016/j.ijpharm.2017.05.002

The three Endonuclease III variants of Deinococcus radiodurans possess distinct and complementary DNA repair activities

Autoren: Aili Sarre, Meike Stelter, Filipe Rollo, Salvatore De Bonis, Anna Seck, Cécilia Hognon, Jean-Luc Ravanat, Antonio Monari, François Dehez, Elin Moe, Joanna Timmins
Veröffentlicht in: DNA Repair, Ausgabe 78, 2019, Seite(n) 45-59, ISSN 1568-7864
Herausgeber: Elsevier BV
DOI: 10.1016/j.dnarep.2019.03.014

A putative siderophore-interacting protein from the marine bacterium Shewanella frigidimarina NCIMB 400: cloning, expression, purification, crystallization and X-ray diffraction analysis

Autoren: Inês B. Trindade, Bruno M. Fonseca, Pedro M. Matias, Ricardo O. Louro, Elin Moe
Veröffentlicht in: Acta Crystallographica Section F Structural Biology Communications, Ausgabe 72/9, 2016, Seite(n) 667-671, ISSN 2053-230X
Herausgeber: John Wiley and Sons Ltd
DOI: 10.1107/s2053230x16011419

A Hydrophilic Channel Is Involved in Oxidative Inactivation of a [NiFeSe] Hydrogenase

Autoren: Sónia Zacarias, Adriana Temporão, Melisa del Barrio, Vincent Fourmond, Christophe Léger, Pedro M. Matias, Inês A. C. Pereira
Veröffentlicht in: ACS Catalysis, Ausgabe 9/9, 2019, Seite(n) 8509-8519, ISSN 2155-5435
Herausgeber: American Chemical Society
DOI: 10.1021/acscatal.9b02347

Structure of Escherichia coli Flavodiiron Nitric Oxide Reductase

Autoren: Célia V. Romão, João B. Vicente, Patrícia T. Borges, Bruno L. Victor, Pedro Lamosa, Elísio Silva, Luís Pereira, Tiago M. Bandeiras, Cláudio M. Soares, Maria A. Carrondo, David Turner, Miguel Teixeira, Carlos Frazão
Veröffentlicht in: Journal of Molecular Biology, Ausgabe 428/23, 2016, Seite(n) 4686-4707, ISSN 0022-2836
Herausgeber: Academic Press
DOI: 10.1016/j.jmb.2016.10.008

PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination

Autoren: Thomas L. Clarke, Maria Pilar Sanchez-Bailon, Kelly Chiang, John J. Reynolds, Joaquin Herrero-Ruiz, Tiago M. Bandeiras, Pedro M. Matias, Sarah L. Maslen, J. Mark Skehel, Grant S. Stewart, Clare C. Davies
Veröffentlicht in: Molecular Cell, Ausgabe 65/5, 2017, Seite(n) 900-916.e7, ISSN 1097-2765
Herausgeber: Cell Press
DOI: 10.1016/j.molcel.2017.01.019

Bioenergetic relevance of hydrogen sulfide and the interplay between gasotransmitters at human cystathionine β-synthase

Autoren: João B. Vicente, Francesca Malagrinò, Marzia Arese, Elena Forte, Paolo Sarti, Alessandro Giuffrè
Veröffentlicht in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Ausgabe 1857/8, 2016, Seite(n) 1127-1138, ISSN 0005-2728
Herausgeber: Elsevier BV
DOI: 10.1016/j.bbabio.2016.03.030

The direct role of selenocysteine in [NiFeSe] hydrogenase maturation and catalysis

Autoren: Marta C Marques, Cristina Tapia, Oscar Gutiérrez-Sanz, Ana Raquel Ramos, Kimberly L Keller, Judy D Wall, Antonio L De Lacey, Pedro M Matias, Inês A C Pereira
Veröffentlicht in: Nature Chemical Biology, Ausgabe 13/5, 2017, Seite(n) 544-550, ISSN 1552-4450
Herausgeber: Nature Publishing Group
DOI: 10.1038/nchembio.2335

The rice cold-responsive calcium-dependent protein kinase OsCPK17 is regulated by alternative splicing and post-translational modifications

Autoren: M. Cecília Almadanim, Nuno M. Gonçalves, Margarida T.G. Rosa, Bruno M. Alexandre, André M. Cordeiro, Mafalda Rodrigues, Nelson J.M. Saibo, Cláudio M. Soares, Célia V. Romão, M. Margarida Oliveira, Isabel A. Abreu
Veröffentlicht in: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Ausgabe 1865/2, 2018, Seite(n) 231-246, ISSN 0167-4889
Herausgeber: Elsevier BV
DOI: 10.1016/j.bbamcr.2017.10.010

Desulfovibrio vulgaris CbiK P cobaltochelatase: evolution of a haem binding protein orchestrated by the incorporation of two histidine residues

Autoren: Susana A.L. Lobo, Marco A.M. Videira, Isabel Pacheco, Mark N. Wass, Martin J. Warren, Miguel Teixeira, Pedro M. Matias, Célia V. Romão, Lígia M. Saraiva
Veröffentlicht in: Environmental Microbiology, Ausgabe 19/1, 2016, Seite(n) 106-118, ISSN 1462-2912
Herausgeber: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.13479

Electron Accepting Units of the Diheme Cytochrome c TsdA, a Bifunctional Thiosulfate Dehydrogenase/Tetrathionate Reductase

Autoren: Julia M. Kurth, José A. Brito, Jula Reuter, Alexander Flegler, Tobias Koch, Thomas Franke, Eva-Maria Klein, Sam F. Rowe, Julea N. Butt, Kevin Denkmann, Inês A. C. Pereira, Margarida Archer, Christiane Dahl
Veröffentlicht in: Journal of Biological Chemistry, Ausgabe 291/48, 2016, Seite(n) 24804-24818, ISSN 0021-9258
Herausgeber: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.m116.753863

Optimizing Electroactive Organisms: The Effect of Orthologous Proteins

Autoren: Bruno M. Fonseca, Luís Silva, Inês B. Trindade, Elin Moe, Pedro M. Matias, Ricardo O. Louro, Catarina M. Paquete
Veröffentlicht in: Frontiers in Energy Research, Ausgabe 7, 2019, ISSN 2296-598X
Herausgeber: Frontiers in Energy Research
DOI: 10.3389/fenrg.2019.00002

A Decade of Biochemical and Structural Studies of the DNA Repair Machinery of Deinococcus radiodurans: Major Findings, Functional and Mechanistic Insight and Challenges

Autoren: Joanna Timmins, Elin Moe
Veröffentlicht in: Computational and Structural Biotechnology Journal, Ausgabe 14, 2016, Seite(n) 168-176, ISSN 2001-0370
Herausgeber: ELSEVIER
DOI: 10.1016/j.csbj.2016.04.001

Clickable 4-Oxo-β-lactam-Based Selective Probing for Human Neutrophil Elastase Related Proteomes

Autoren: Eduardo F. P. Ruivo, Lídia M. Gonçalves, Luís A. R. Carvalho, Rita C. Guedes, Stefan Hofbauer, José A. Brito, Margarida Archer, Rui Moreira, Susana D. Lucas
Veröffentlicht in: ChemMedChem, Ausgabe 11/18, 2016, Seite(n) 2037-2042, ISSN 1860-7179
Herausgeber: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/cmdc.201600258

A novel bacterial class V dye-decolourizing peroxidase from the extremophile Deinococcus radiodurans : cloning, expression optimization, purification, crystallization, initial characterization and X-ray diffraction analysis

Autoren: Kelly Stefany Tuna Frade, Andreia Cecília Pimenta Fernandes, Celia Marisa Silveira, Carlos Frazão, Elin Moe
Veröffentlicht in: Acta Crystallographica Section F Structural Biology Communications, Ausgabe 74/7, 2018, Seite(n) 419-424, ISSN 2053-230X
Herausgeber: John Wiley and Sons Ltd
DOI: 10.1107/s2053230x18008488

Insights into the Structures of Superoxide Reductases from the Symbionts Ignicoccus hospitalis and Nanoarchaeum equitans

Autoren: Célia V. Romão, Pedro M. Matias, Cristiana M. Sousa, Filipa G. Pinho, Ana F. Pinto, Miguel Teixeira, Tiago M. Bandeiras
Veröffentlicht in: Biochemistry, Ausgabe 57/36, 2018, Seite(n) 5271-5281, ISSN 0006-2960
Herausgeber: American Chemical Society
DOI: 10.1021/acs.biochem.8b00334

Continuous flexibility analysis of SARS-CoV-2 spike prefusion structures

Autoren: R. Melero, C.O.S. Sorzano, B. Foster, J.L. Vilas, M. Martínez, R. Marabini, E. Ramírez-Aportela, R. Sánchez-García, D. Herreros, L. del Caño, P. Losana, Y. Fonseca-Reyna, P. Conesa, D. Wrapp, P. Chacón, J.S. McLellan, H.D. Tagare, J.M. Carazo
Veröffentlicht in: IUCrJ, Ausgabe 7, 2020, Seite(n) 1059-1069, ISSN 2052-2525
Herausgeber: International Union of Crystallography
DOI: 10.1107/s2052252520012725

Advances in Xmipp for Cryo-electron Microscopy: From Xmipp to Scipion

Autoren: D. Strelak, A. Jiménez-Moreno, J.L. Vilas, E. Ramírez-Aportela, R. Sánchez-García, D. Maluenda, J. Vargas, D. Herreros, E. Fernández-Giménez, F. de Isidro-Gómez, J. Horacek, D. Myska, M. Horacek, P. Conesa, Y.C. Fonseca-Reyna, J. Jiménez, M. Martínez, M. Harastani, S. Jonic, J. Filipovic, R. Marabini, J.M. Carazo, C.O.S. Sorzano.
Veröffentlicht in: Molecules, Ausgabe 26 / 20, 2021, Seite(n) 6224, ISSN 1420-3049
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules26206224

Image processing tools for the validation of CryoEM maps

Autoren: C.O.S. Sorzano, J.L. Vilas, E. Ramírez-Aportela, D. del Hoyo, D. Herreros, E. Fernandez-Giménez, D. Marchán, F. de Isidro Gómez, J.R. Macías, I. Sánchez, L. del Caño, Y. Fonseca-Reyna, P. Conesa, A. García-Mena, J. Burguet, J. García Condado, J. Méndez García, M. Martínez, A. Muñoz Barrutia, R. Marabini, J. Vargas, J.M. Carazo.
Veröffentlicht in: Faraday Discussions, Ausgabe 240, 2022, Seite(n) 210-227, ISSN 1359-6640
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/d2fd00059h

Book chapters (1)

Cytochrome bd and Gaseous Ligands in Bacterial Physiology

Autoren: Elena Forte, Vitaliy B. Borisov, João B. Vicente, Alessandro Giuffrè
Veröffentlicht in: Advances in Microbial Physiology volume 71, Ausgabe 71, 2017, Seite(n) 171-234, ISBN 9780-128123850
Herausgeber: Elsevier
DOI: 10.1016/bs.ampbs.2017.05.002