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Unravelling Glycochemistry with Ion Mobility Spectrometry and Gas-Phase Spectroscopy

Objective

Oligosaccharides or glycans are essential in nature and central participants in virtually every biological process. The extensive structural diversity enables glycans to encode rich information in biological functions; however, it also creates major challenges in almost all aspects of the glycosciences. The synthetic formation of glycosidic bonds during glycan assembly for example, is mechanistically still not fully understood. This is largely a result of highly-reactive, but short-lived oxocarbenium ion intermediates, which are difficult to study using established techniques. However, the structure of these intermediates dictates the stereochemistry of the resulting glycosidic bond, the control of which is absolutely crucial for a successful synthesis. An equally important aspect is the sequencing of glycans, which is generally complicated by the frequent occurrence of isomers. This is further complicated by poorly understood rearrangement reactions during mass spectrometric analysis, which often lead to erroneous structural assignments. Here as well, cationic intermediates are the key species that determine the outcome of the rearrangement.
The aim of GlycoSpec is to unravel fundamental aspects of oligosaccharide reaction and fragmentation mechanisms by probing cationic glycan intermediates. To do so, a unique combination of instrument and method development (ion mobility-mass spectrometry and cold-ion spectroscopy), chemical synthesis and theory will be used. The gain in mechanistic understanding will provide the basis to tailor building blocks and reaction conditions and is expected to lead to a major advancement in glycosynthesis. The mechanistic understanding of fragmentation reactions will furthermore lead to general rules for the prediction of tandem mass spectra. Just like in the early years of proteomics, this will make the technology automatable and accessible for broader applications. Therefore, GlycoSpec will help to initiate the pursuit of glycomics.

Field of science

  • /natural sciences/chemical sciences/analytical chemistry/spectroscopy
  • /natural sciences/biological sciences/biochemistry/biomolecules/carbohydrates

Call for proposal

ERC-2019-COG
See other projects for this call

Funding Scheme

ERC-COG - Consolidator Grant

Host institution

FREIE UNIVERSITAET BERLIN
Address
Kaiserswerther Strasse 16-18
14195 Berlin
Germany
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 705 350

Beneficiaries (2)

FREIE UNIVERSITAET BERLIN
Germany
EU contribution
€ 1 705 350
Address
Kaiserswerther Strasse 16-18
14195 Berlin
Activity type
Higher or Secondary Education Establishments
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Germany
EU contribution
€ 937 500
Address
Hofgartenstrasse 8
80539 Muenchen
Activity type
Research Organisations