Description du projet
Une première version du Human Cell Atlas pour le pancréas
Les maladies affectant le pancréas incluent l’adénocarcinome pancréatique, un cancer dévastateur, et la pancréatite, un trouble pouvant menacer la vie des patients atteints. Le diabète constitue une pathologie chronique très répandue qui touche le pancréas. L’objectif du consortium ESPACE financé par l’UE est de mettre au point la première version du Human Cell Atlas (HCA) pour le pancréas. La recherche intégrera les profils moléculaires, ainsi que les données des transcriptomes et de la recherche épigénomique de plus d’un million de cellules uniques, avec les données protéomiques des tissus, afin de répondre aux questions fondamentales de phénotype des cellules, tant pour les adultes sains que pendant le développement embryonnaire. ESPACE se focalisera sur le diabète de type 2 dans le cadre de son initiative HCA vers une meilleure compréhension des maladies du pancréas. La création du tout premier atlas complet et unique en son genre du pancréas humain permettra de faire avancer les méthodes et les normes d’importance cruciales pour le projet HCA et également pour d’autres organes.
Objectif
The human pancreas is a physiologically unique organ involved in the secretion of several hormones, such as insulin, thus regulating blood sugar levels, as well as the secretion of enzymes for the digestive system. Several human diseases are associated with the pancreas: Pancreatic andenocarcinoma is one of the most aggressive cancers in humans and pancreatitis is a common potentially life-threatening disorder related to adverse lifestyle conditions. A very common chronical disease affecting the pancreas is Diabetes mellitus, a metabolic disorder that is characterised by high blood sugar levels due to the lack of insulin produced in the pancreas. Despite its high physiological importance, the high autolytic activity makes the pancreas a challenging tissue to study. The Expression and Spatial analysis Pancreas Atlas Consortium Europe, ESPACE, has formed as merger of three prior Human Cell Atlas (HCA) early pilot studies of the pancreas funded by the Chan Zuckerberg initiative. Based on the there developed methods and standards for sample procurement, single cell profiling, spatial proteomics, and computational pipelines we will here build a first version of the Human Cell Atlas of the Pancreas. We will integrate molecular profiles, including transcriptome and epigenomics data from more than 1 million single cells jointly together with tissue proteomics allowing to address fundamental questions of cell state and phenotype in healthy adults and during foetal development. Thus, we will provide a first version of an open single cell repository of the pancreas built upon the Data Coordination Platform of the HCA. Exploring the benefit of the HCA of the pancreas for tackling pancreatic diseases, ESPACE will reach out into types 2 diabetes. Besides creating a unique and first of its kind comprehensive atlas of the human pancreas the methods and standards developed by ESPACE will be of high relevance for HCA projects in other organs.
Champ scientifique
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomics
- medical and health sciencesclinical medicineendocrinologydiabetes
- medical and health sciencesclinical medicineoncology
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
- natural sciencesbiological sciencesgeneticsepigenetics
Programme(s)
Régime de financement
RIA - Research and Innovation actionCoordinateur
10117 Berlin
Allemagne