Human Gonad Developmental Cell Atlas

Project Information

HUGODECA

Grant agreement ID: 874741

DOI 10.3030/874741

Project closed

EC signature date 24 November 2019

Start date 1 January 2020

End date 30 June 2022

Funded under

• SOCIETAL CHALLENGES - Health, demographic change and well-being

Total cost € 4 822 198,75

EU contribution € 4 822 197,00

Coordinated by

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
France

Deliverables

Documents, reports (11)

Profiling with NanoString WTA technology

A data set containing spatial coordinates of germ and somatic cells

3D gonad cell maps

A 3D spatial map of gonadal cells in male and female human embryos

2D ST gonad data set

A data set containing gene expression matrices linked to their spatial positions within Hematoxylin and Eosin HE stained developmental gonad tissue sections

2D ISS gonad data set

A data set containing gene expression images in human developmental gonad tissue sections

3D integrated image data

3D image datasets integrated in 3D models of whole developmental gonads

2D gonad cell map

A 2D ultrahigh content cellular and protein expression map of gonadal cells in male and female human embryos

Imaging mass cytometry analysis of developing gonads

Imaging mass data of human gonads during sex determination

3D integrated spatial OMICS data

Spatial OMICS data sets integrated in 3D models of whole developmental gonads

ISS profiling in cleared tissues

Protocols for generating spatial ISS expression data in optically cleared tissues

Integration of spatial and scRNA/scATAC-seq data

Computational integration of spatial and scRNAATACseq datasets

ST profiling in cleared tissues

Protocols for generating ST expression data in optically cleared tissues

Websites, patent fillings, videos etc. (1)

The HUGODECA web-portal

A centralized web-resource to provide access to multi-omics data from the HUGODECA project

Other (3)

Communication and Dissemination tools

This deliverable is composed of: the web site, the graphic charter, the project leaflet.

Final public symposium on sex determination differences, human reproduction and gender
Training courses on Spatial transcriptomics and 3D imaging/tissue clearing

Publications

Peer reviewed articles (2)

Deciphering Sex-Specific Differentiation of Human Fetal Gonads: Insight From Experimental Models

Author(s): Malene Lundgaard Riis, Anne Jørgensen
Published in: Frontiers in Cell and Developmental Biology, Issue 2296634x, 2022, ISSN 2296-634x
Publisher: Frontiers in Cell and Developmental Biology
DOI: 10.3389/fcell.2022.902082

Single-cell roadmap of human gonadal development

Author(s): Luz Garcia-Alonso, Valentina Lorenzi, Cecilia Icoresi Mazzeo, João Pedro Alves-Lopes, Kenny Roberts, Carmen Sancho-Serra, Justin Engelbert, Magda Marečková, Wolfram H Gruhn, Rachel A Botting, Tong Li, Berta Crespo, Stijn van Dongen , Vladimir Kiselev, Elena Prigmore, Mary Herbert, Ashley Moffett, Alain Chedotal, Omer Ali Bayraktar, Azim Surani, Muzlifah Haniffa, Roser Vento-Tormo
Published in: Nature, Issue 14764687, 2022, ISSN 1476-4687
Publisher: Nature
DOI: 10.1038/s41586-022-04918-4