Enantioselective C-H Oxidation Guided by Rational Catalyst Design

Projektinformationen

ECHO-GRACADE

ID Finanzhilfevereinbarung: 883922

DOI

10.3030/883922

EK-Unterschriftsdatum 4 Mai 2020

Startdatum 1 Januar 2021

Enddatum 31 Dezember 2026

Finanziert unter

• EXCELLENT SCIENCE - European Research Council (ERC)

Gesamtkosten

€ 2 499 387,50

EU-Beitrag

€ 2 499 387,50

Koordiniert durch

UNIVERSITAT DE GIRONA
Spain

Periodic Reporting for period 3 - ECHO-GRACADE (Enantioselective C-H Oxidation Guided by Rational Catalyst Design)

Berichtszeitraum: 2024-01-01 bis 2025-06-30

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

Despite its huge potential in organic synthesis, non-enzymatic enantioselective C-H oxidation of aliphatic sites remains inaccessible and has never been incorporated in synthesis. The huge potential of aliphatic C-H oxidation reactions has fuelled extensive exploration of novel methods over the last decade, where the challenge posed by the relatively inert nature of the aliphatic C-H bond has been addressed by using photo-, electro-chemically or
thermally-generated radical and radical–like reagents. These reagents cleave the C-H bond via a hydrogen atom transfer (HAT) process to form a substrate radical. Two main limitations exist; firstly, in the absence of directing groups, siteselectivity is dictated and limited by the innate relative HAT reactivity of the different C-H bonds in the substrate, irrespective of the reagent. A second long-standing limitation is that strong aliphatic C-H bonds are not accessible in the presence of conventional functional groups, such as alcohols, amines, ethers or amides, which strongly activate the adjacent C-H bonds towards oxidation. Chemo-selectivity is thus a problem that imposes the use of protecting-deprotecting sequences. Progress has been made to address these aspects only to a limited extent. However, the most challenging and as yet insurmountable problems with existing methodologies are encountered for realizing enantioselective C-H oxidations.

Considering these premises, the current project targets the design and development of chiral C-H oxidation catalysts in order to set enantioselective aliphatic C-H oxidation as a reliable tool in organic synthesis. This will be demonstrated by proof-of-principle realization of four classes of chiral aliphatic CH oxidation reactions; a) desymmetrization of cyclic substrates via enantioselective C-H oxidation, b) lactonization via an initial enantioselective C-H hydroxylation, c) atropoenantioselective C-H oxidation of biaryls and d) site- and enantioselective desymmetrizations of primary alkyl amines guided by remote supramolecular recognition. Of note, with the single exception of an initial report from the PI team, none of the four chiral reactions has previously been described with non-enzymatic methodologies.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

The project is organized in five working packages (WP’s). The first one (WP1) is devoted to catalyst design and evolution. The fourth following working packages (WP2-5) focus on a specific class of reactions where asymmetric C-H oxidation is applied and developed. The evolution of the project is planned in nine phases. The three first phases are scheduled for the current reporting period and the progress is detailed in the following lines;
• Phase 1 entails preparation of a training set of catalysts and model substrates to be employed in the first set of model reactions, corresponding to WP2-5, that will serve as the starting point for a multiparametric analysis in order to identify selectivity defining parameters. Catalysts initially planed for addressing WP's 2-4 have been prepared and characterized. Catalysts corresponding to WP5 are under development.
• Phases 2 and 3 entail performing the first set of catalytic model reactions on enantioselective C-H oxidation with the training set of catalysts, and consequently the elaboration of a matrix with selectivity parameters derived from catalyst and substrate chemical and 3D structure.
• Phase 4 entails building multiparametric models that identify key aspects that govern the enantioselectivity in the model reactions. This analysis has been performed so far for four asymmetric reactions and models have been extracted that are currently under validation (Phase 5).
• Phase 6 entails designing a new generation of catalysts guided from the models validated in Phase 5, which will be prepared in Phase 7. Preparation of two novel generations of catalysts has been performed.
• Phase 8 entails testing and development of substrate scope for the new generation of catalysts. The phase is underway
• Phase 9 will focus in using the catalysts in relevant targets. The phase has been initiated

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

The selective oxidation of non-activated aliphatic C–H bonds is a very powerful and useful reaction because it transforms these poorly reactive bonds, ubiquitous in organic molecules, into chemically reactive C-O bonds, amenable for diverse chemical elaboration. Enantioselective C–H bond oxidations are particularly difficult because reagents that are both chiral and capable of oxidizing the inherently strong aliphatic C–H bonds (BDE > 95 Kcal.mol-1) are rare, and the facile overoxidation of the first formed secondary alcohol products, resulting from hydroxylation of a methylenic site, into ketone eliminates the chirality. As a consequence, enantioselective aliphatic C–H bond oxidation is only accessible via enzymatic methods and remains a non solved reaction in conventional organic synthesis. Enantioselective oxidation of non-ativated C-H bonds is currently being performed by enzymes, generally metalloenzymes that generate high valent iron oxo reactive species. State of the art non -enzymatic C-H oxidation methods operate in activated C-H bonds (adjacent to heteroatom, allyllic or benzylic) and generally require the use of large excess of substrate to minimize overoxidation reactions.
The ECHO-GRACADE project addresses the problem of C-H oxidation in order to convert the reaction in a powerful tool in organic synthesis. It does so by;
a) Developing new families of powerful but oxidatively robust chiral oxidation catalysts based on earth abundant metals, which generate reactive metal-oxo species that selectively attack aliphatic C-H bonds. The chiral nature of the catalysts convey them with the capability of introducing chirality in the C-H oxidation reaction.
b) Catalyst development, pursued via multiparametric analysis of four model reactions. Catalyst evolution will lead to catalysts to address the four classes of reactions, which have no current solution.
c) Developing four classes of general reactions that entail an initial C-H clavage by a metal-oxo species will be developed in highly enantiomeric form.