Structural Biology of Human Ribosome Biogenesis

Project Information

Human-Ribogenesis

Grant agreement ID: 885711

DOI

10.3030/885711

Project closed

EC signature date 18 September 2020

Start date 1 October 2020

End date 30 September 2025

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 080 137,50

EU contribution

€ 2 080 137,50

Coordinated by

LUDWIG-MAXIMILIANS-UNIVERSITAET MUENCHEN
Germany

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (19)

Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S

Author(s): Lau B, Huang Z, Kellner N, Niu S, Berninghausen O, Beckmann R, Hurt E, Cheng J.
Published in: EMBO Reports, 2023, ISSN 1469-221X
Publisher: Nature Publishing Group
DOI: 10.15252/embr.202356910

Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release

Author(s): Benjamin Lau; Olga Beine-Golovchuk; Markus Kornprobst; Jingdong Cheng; Dieter Kressler; Beáta Jády; Tamás Kiss; Roland Beckmann; Ed Hurt
Published in: Cell Reports, Issue 1, 2022, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2022.111684

Structure of nascent 5S RNPs at the crossroad between ribosome assembly and MDM2-p53 pathways

Author(s): Nestor Miguel Castillo Duque de Estrada, Matthias Thoms, Dirk Flemming, Henrik M Hammaren, Robert Buschauer, Michael Ameismeier, Jochen Baßler, Martin Beck, Roland Beckmann, Ed Hurt
Published in: Nature Structural and Molecular Biology, 2023, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-023-01006-7

Highly conserved ribosome biogenesis pathways between human and yeast revealed by the MDN1-NLE1 interaction and NLE1 containing pre-60S subunits

Author(s): Federica Fiorentino, Matthias Thoms, Klemens Wild, Timo Denk, Jingdong Cheng, Jakub Zeman, Irmgard Sinning, Ed Hurt, Roland Beckmann
Published in: Nucleic Acids Research, Issue 53, 2025, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaf255

Multiprotein bridging factor 1 is required for robust activation of the integrated stress response on collided ribosomes

Author(s): Kyusik Q. Kim, Jeffrey J. Li, Ankanahalli N. Nanjaraj Urs, Miguel E. Pacheco, Victor Lasehinde, Timo Denk, Petr Tesina, Shota Tomomatsu, Yoshitaka Matsuo, Elesa McDonald, Roland Beckmann, Toshifumi Inada, Rachel Green, Hani S. Zaher
Published in: Molecular Cell, Issue 84, 2024, Page(s) 4594-4611.e9, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2024.10.029

Mechanistic insights into Bcs1-mediated mitochondrial membrane translocation of the folded Rieske protein

Author(s): Cristian Rosales-Hernandez, Matthias Thoms, Otto Berninghausen, Thomas Becker, Roland Beckmann
Published in: The EMBO Journal, Issue 44, 2025, Page(s) 3720-3741, ISSN 1460-2075
Publisher: Springer Science and Business Media LLC
DOI: 10.1038/s44318-025-00459-4

Molecular basis for recognition and deubiquitination of 40S ribosomes by Otu2.

Author(s): Ikeuchi K, Ivic N, Buschauer R, Cheng J, Fröhlich T, Matsuo Y, Berninghausen O, Inada T, Becker T, Beckmann R.
Published in: Nature Communications, 2023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-38161-w

A distinct assembly pathway of the human 39S late pre-mitoribosome

Author(s): Jingdong Cheng; Otto Berninghausen; Roland Beckmann
Published in: Nature Communications, Issue 1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-24818-x

SnapShot: Eukaryotic ribosome biogenesis I.

Author(s): Hurt E, Cheng J, Baβler J, Iwasa J, Beckmann R.
Published in: Cell, 2023, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2023.04.030

H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation

Author(s): Paulina Fischer, Matthias Thoms, Benjamin Lau, Timo Denk, Maria Kuvshinova, Otto Berninghausen, Dirk Flemming, Ed Hurt, Roland Beckmann
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-025-59656-8

UFMylation orchestrates spatiotemporal coordination of RQC at the ER

Author(s): Ivan Penchev, Samantha Gumbin, Francesco Scavone, Otto Berninghausen, Thomas Becker, Ron Kopito, Roland Beckmann
Published in: Science Advances, Issue 11, 2025, ISSN 2375-2548
Publisher: American Association for the Advancement of Science (AAAS)
DOI: 10.1126/sciadv.adv0435

Structural basis for differential inhibition of eukaryotic ribosomes by tigecycline

Author(s): Xiang Li, Mengjiao Wang, Timo Denk, Robert Buschauer, Yi Li, Roland Beckmann, Jingdong Cheng
Published in: Nature Communications, Issue 15, 2024, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-024-49797-7

In vitro structural maturation of an early stage pre-40S particle coupled with U3 snoRNA release and central pseudoknot formation

Author(s): Cheng J, La Venuta G, Lau B, Berninghausen O, Beckmann R, Hurt E.
Published in: Nucleic Acids Research, Issue 13624962, 2022, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/nar/gkac910

Emergence of the primordial pre-60S from the 90S pre-ribosome

Author(s): Ismail S, Flemming D, Thoms M, Gomes-Filho JV, Randau L, Beckmann R, Hurt E.
Published in: Cell Reports, 2022, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2022.110640

Concurrent remodelling of nucleolar 60S subunit precursors by the Rea1 ATPase and Spb4 RNA helicase

Author(s): Matthias Thoms; Valentin Mitterer; Robert Buschauer; Otto Berninghausen; Ed Hurt; Roland Beckmann
Published in: eLife, Issue 1, 2023, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.1101/2022.11.14.516466

The nucleoplasmic phase of pre-40S formation prior to nuclear export

Author(s): Jingdong Cheng; Benjamin Lau; Matthias Thoms; Michael Ameismeier; Otto Berninghausen; Ed Hurt; Roland Beckmann
Published in: Nucleic Acids Research, Issue 1, 2022, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/nar/gkac961

Structural insights into coordinating <scp>5S RNP</scp> rotation with <scp>ITS2</scp> pre‐<scp>RNA</scp> processing during ribosome formation

Author(s): Matthias Thoms, Benjamin Lau, Jingdong Cheng, Lisa Fromm, Timo Denk, Nikola Kellner, Dirk Flemming, Paulina Fischer, Laurent Falquet, Otto Berninghausen, Roland Beckmann, Ed Hurt
Published in: EMBO reports, Issue 24, 2023, ISSN 1469-221X
Publisher: Nature Publishing Group
DOI: 10.15252/embr.202357984

UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER

Author(s): Paul A. DaRosa, Ivan Penchev, Samantha C. Gumbin, Francesco Scavone, Magda Wąchalska, Joao A. Paulo, Alban Ordureau, Joshua J. Peter, Yogesh Kulathu, J. Wade Harper, Thomas Becker, Roland Beckmann, Ron R. Kopito
Published in: Nature, Issue 627, 2024, Page(s) 445-452, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-024-07073-0

SnapShot: Eukaryotic ribosome biogenesis II

Author(s): Ed Hurt, Janet Iwasa, Roland Beckmann
Published in: Cell, Issue 187, 2025, Page(s) 1314-1314.e1, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2024.01.043

Non-peer reviewed articles (1)

Structural basis for the final steps of human 40S ribosome maturation

Author(s): Michael Ameismeier, Zemp I, van den Heuvel J, Matthias Thoms, Berninghausen O, Kutay U, Beckmann R.
Published in: Nature, Issue 00280836, 2020, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-020-2929-x