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Physiological, biochemical and transcriptomic responses to salinity excess in the seagrass Posidonia oceanica provide insights of tolerance mechanisms and tools for environmental biomonitoring

Description du projet

Étudier les effets de la saumure sur le métabolisme du stress des herbes marines protégées Posidonia oceanica

Le dessalement a gagné en importance en tant qu’activité de gestion de l’eau, en particulier dans les pays du littoral méditerranéen. Or, la «saumure» rejetée directement dans la zone subtidale en tant que déchet du processus de dessalement risque de nuire aux communautés côtières, dont la plupart se nourrissent grâce aux prairies sous-marines. Le projet OSMOTIC SEAGRASS, financé par l’UE, entend étudier les effets de la saumure sur le métabolisme du stress de l’herbe marine protégée Posidonia oceanica. Grâce à une série d’expériences réalisées en laboratoire et sur le terrain, l’équipe observera leur métabolisme antioxydant, leur régulation osmotique et l’ensemble du transcriptome afin de les comparer à la physiologie et au métabolisme primaire. Ces travaux permettront de mieux comprendre les mécanismes de tolérance de Posidonia oceanica et ainsi de mettre au point des outils de biosurveillance de l’environnement.

Objectif

Desalinization has become an important water management activity, Especially in countries along the Mediterranean Seashore. Desalination produces a discard of brines which are directly discharged to the subtidal; these can cause detrimental effects on coastal communities, most of which are nurtured by seagrass meadows. Posidonia oceanica is a protected seagrass, base of the most ecologically and economically important ecosystems along the Mediterranean coast. Our project aims to assess the effects of brines on the stress metabolism of P. oceanica through laboratory- and field-based experiments. We aim to provide insights of tolerance mechanisms through observations on antioxidant metabolism, osmotic regulation and the whole transcriptome; this will be contrasted with observations on the physiology and primary metabolism. Laboratory experiments can provide valuable information on specific metabolic features but do not necessarily represent responses at the natural, more complex, environment; in contrast, field observations denote responses under realistic conditions but lack information that can be attributed to specific stressors. In this context, the latter will provide valuable information on mechanisms to thrive under hypersalinity and contribute to study biomarkers that could act as environmental biotechnology tools to follow the extent of brine impacts. The research will be led Dr. Claudio Sáez, experienced researcher in the field of biochemical and molecular stress metabolism. Groups of Prof. José Luis Sánchez-Lizaso at Universidad de Alicante (beneficiary) and Dr. Juan Manuel Ruiz at the Spanish Oceanographic Institute (secondment), in addition to the Spanish Association of Desalination and Reuse (industry link) through Dr. Domingo Zarzo, will support the researcher to develop this interdisciplinary project that merges the expertise of highly achieved scientists in the areas of ecology, physiology, biochemistry, transcriptomics and innovation.

Coordinateur

UNIVERSIDAD DE ALICANTE
Contribution nette de l'UE
€ 172 932,48
Adresse
CAMPUS DE SAN VICENTE RASPEIG
03690 Alicante
Espagne

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Région
Este Comunitat Valenciana Alicante/Alacant
Type d’activité
Higher or Secondary Education Establishments
Liens
Coût total
€ 172 932,48