Artificial microtubules based on switchable cyclic peptides

Projektbeschreibung

Neuartige lebensechte Materialien

Mikrotubuli sind Teil des Zellzytoskeletts und können sich je nach den Anforderungen der Zelle auf- und abbauen. Die Materialwissenschaft hat versucht, diese Fähigkeit von Mikrotubuli zur Selbstorganisation nachzuahmen, indem künstliche supramolekulare Polymere entwickelt wurden, die jedoch hinsichtlich ihrer mechanischen Eigenschaften hinterherhinken. Um eine Lösung zu finden, werden im Rahmen des EU-finanzierten Projekts CYCLOTUBES zyklische Peptide eingesetzt, die mithilfe von Chemikalien oder Enzymen zwischen dem aufgebauten und dem abgebauten Zustand wechseln können. Die Leistung dieser künstlichen Mikrotubuli wird in einer zellähnlichen Umgebung mikroskopisch überwacht und hat das Potenzial, zu innovativen Materialien mit neuartigen mechanischen Eigenschaften zu führen.

Ziel

Naturally occurring living materials, for e.g. the cell cytoskeleton, consist of intricate supramolecular polymers whose self-assembly is controlled by high energy molecules such as guanosine triphosphate GTP. MiNaturally occurring living materials, such as the cell cytoskeleton, consist of intricate supramolecular polymers whose self-assembly is controlled by high energy molecules such as guanosine triphosphate GTP. Microtubules for example, are incredibly strong, but because they are chemically fueled by GTP, they can be built up or broken down at specific times and locations inside the cell. The tubules are in so-called non-equilibrium steady states, and are kept away from the thermodynamic equilibrium for extended periods of time. In the recent years, artificial supramolecular polymers have been made that are transiently out-of-equilibrium by addition of a single shot of chemical fuel, or for long times by continuous addition of fuel and removal of waste. The mechanical properties of the latter artificial systems are quite poor in comparison with real microtubules. The aim of this CYCLOTUBES project is to make artificial microtubules from cyclic peptides that can chemically or enzymatically be switched between the assembled and disassembled state. To this end, we will use oxidation and reduction reactions in a cell-like environment, that is, in a membrane enclosed chamber. In the latter, chemical fuel can be added and waste be removed continuously. In addition, the assembly/disassembly of the artificial tubules can be monitored using microscopy. Our work will give fundamentally new insights into out-of-equilibrium self-assembly, and could lead to novel life-like materials that are capable of performing significant mechanical work due to the unique mechanical properties of the cyclic peptide tubules. The candidate will work at the forefront of the field of systems chemistry and supramolecular chemistry, which are very important for the competitiveness of Europe.