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High Content Microanalytical Ramanomics for Ultraquantitative Phenotyping of Individual Stem Cells and Bioengineered Organoids

Descripción del proyecto

Una plataforma analítica para elaborar perfiles de organoides

Los organoides se erigen como herramientas prometedoras para el descubrimiento de fármacos y el estudio de la patogenia. Sin embargo, los problemas relacionados con la capacidad de los organoides para reproducir la compleja estructura y función de tejidos y órganos biológicos reales han limitado su uso. Para paliar este problema, los científicos del proyecto UltraRamanomics, financiado con fondos europeos, proponen desarrollar una plataforma bioanalítica para elaborar perfiles fenotípicos de organoides obtenidos a partir de células madre pluripotentes inducidas humanas. Mediante el uso de mediciones bioquímicas basadas en espectroscopia, la plataforma ofrecerá una comparación directa de distintos modelos de organoides con una gran resolución espacial, lo cual llenará la brecha del conocimiento en la investigación de los organoides celulares.

Objetivo

Human induced pluripotent stem (hiPS) cell-based organoids have recently emerged as a promising model system to recapitulate the structure and function of actual biological tissues and organs. These 3D self-organized multi-cellular tissue systems bridge the gap from cell to tissue/organ levels, providing bio-relevant model systems with foreseeable utility in drug discovery, regenerative medicine, and the study of disease pathogenesis, but the increased complexity and unique attributes of each system introduces new challenges in cell culture and experimental reproducibility. While organoids offer profound potential for applications in biological and biomedical research, their scientific value is limited by how closely they mimic the in vivo tissue systems they are intended to model. To achieve a successful organoid-based predictive model system, it is crucial to have a quantitative biochemical and structural analysis technique for single cells and organoids.
In UltraRamanomics, I will develop a robust quantitatively-calibrated bioanalytical platform for high content phenotypic profiling of individual stem cells and hiPS cell-derived organoids, to allow direct structural and quantitative compositional comparison of organoid models in absolute biochemical measurements, thereby addressing the currently unmet analytical need. The platform will be based on micro-Raman spectroscopy – enabling direct measurements of the absolute biochemical composition of single-cells and tissue organoids with unprecedented spatial resolution – to provide a novel analytical solution to the existing gap in stem cell organoid research. This proposal takes full advantage of recent advances in ultraquantitative micro-calibration technology I developed during my PhD, quantitative volumetric Raman imaging (qVRI) methodology developed by the Stevens Group, and the organoid-on-a-chip platform in development at the Centre of Excellence Hybrid Technology Hub with the Krauss Group (Secondment).

Coordinador

IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
Aportación neta de la UEn
€ 212 933,76
Dirección
SOUTH KENSINGTON CAMPUS EXHIBITION ROAD
SW7 2AZ LONDON
Reino Unido

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Región
London Inner London — West Westminster
Tipo de actividad
Higher or Secondary Education Establishments
Enlaces
Coste total
€ 212 933,76