Projektbeschreibung
Eine Analyseplattform zur Profilerstellung von Organoiden
Organoide treten immer häufiger als vielversprechende Mittler für die Wirkstoffsuche und Untersuchung von Krankheitsentstehungen auf den Plan. Doch weil Organoide nur begrenzt fähig sind, die komplexe Struktur und Funktion echter lebendiger Gewebe und Organe abzubilden, kamen sie nie in großem Umfang zum Einsatz. Darum will ein Forschungsteam des EU-finanzierten Projekts UltraRamanomics eine bioanalytische Plattform für die Erstellung phänotypischer Profile von Organoiden entwickeln, die mit einzelnen Stammzellen und induzierten pluripotenten Stammzellen von Menschen arbeitet. Anhand biochemischer Messungen aus spektroskopischen Untersuchungen liefert die Plattform mit hervorragender räumlicher Auflösung einen direkten Vergleich verschiedener Organoidmodelle und schließt so eine Wissenslücke in der Organoidforschung an Stammzellen.
Ziel
Human induced pluripotent stem (hiPS) cell-based organoids have recently emerged as a promising model system to recapitulate the structure and function of actual biological tissues and organs. These 3D self-organized multi-cellular tissue systems bridge the gap from cell to tissue/organ levels, providing bio-relevant model systems with foreseeable utility in drug discovery, regenerative medicine, and the study of disease pathogenesis, but the increased complexity and unique attributes of each system introduces new challenges in cell culture and experimental reproducibility. While organoids offer profound potential for applications in biological and biomedical research, their scientific value is limited by how closely they mimic the in vivo tissue systems they are intended to model. To achieve a successful organoid-based predictive model system, it is crucial to have a quantitative biochemical and structural analysis technique for single cells and organoids.
In UltraRamanomics, I will develop a robust quantitatively-calibrated bioanalytical platform for high content phenotypic profiling of individual stem cells and hiPS cell-derived organoids, to allow direct structural and quantitative compositional comparison of organoid models in absolute biochemical measurements, thereby addressing the currently unmet analytical need. The platform will be based on micro-Raman spectroscopy – enabling direct measurements of the absolute biochemical composition of single-cells and tissue organoids with unprecedented spatial resolution – to provide a novel analytical solution to the existing gap in stem cell organoid research. This proposal takes full advantage of recent advances in ultraquantitative micro-calibration technology I developed during my PhD, quantitative volumetric Raman imaging (qVRI) methodology developed by the Stevens Group, and the organoid-on-a-chip platform in development at the Centre of Excellence Hybrid Technology Hub with the Krauss Group (Secondment).
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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Koordinator
SW7 2AZ LONDON
Vereinigtes Königreich