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Role of SIK3 in energy homeostasis

Project description

Investigating the role of the SIK3 gene in energy homeostasis

Obesity occurs as the result of an imbalance between food intake and energy expenditure. The EU-funded ObeSIK3 project will bring a new perspective to the understanding of obesity by studying a splice site mutation in the salt-inducible kinase 3 (SIK3) gene. This gene mutation is likely to disrupt energy homeostasis since mice with an exon 13 mutation of the SIK3 gene present an obese phenotype. To investigate how SIK3 affects energy homeostasis, the project will use virogenetic approaches in SIK3–exon 13 floxed mice and generate a neuron-specific mutant mice line for exon 13. Investigating what contributes to the pathophysiology of obesity increases the chances of finding efficient therapeutic targets for treating this major health issue.

Objective

In the last decades, obesity has become a growing health burden hence the importance of understanding its pathophysiology and the need to search for new therapeutic targets. Yanagisawa/Funato Lab (Y/F Lab) has recently discover that a splicing mutation in the Salt-inducible kinase 3 (Sik3) gene is involved in determining sleep need , and as well in a likely defective energy homeostasis since mice mutant for exon 13 of the Sik3 gene present an obese phenotype. The main objective of my proposed action is to identify the role of SIK3 in the central nervous system (CNS) in the regulation of energy balance and study its suitability as a target to develop an anti-obesity therapy. To achieve this, I will use virogenetic approaches in Sik3-ex13 flox mice and a neuron-specific mutant mice line for exon 13 of Sik3 gene will be generated. The molecular characterization of the animal models will be conducted by using several cutting-edge techniques such as RNA-Seq and phosphoproteomics. The identified targets will be functionally analyzed using genetics approaches. In sum, by doing the proposed project I will expect to uncover the influence of a potential new sensor in metabolic homeostasis. Moreover, this work in combination with the one going already on in Y/F Lab in relation to sleep may allow to understand the interrelationship between sleep disorders and obesity.

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Programme(s)

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Topic(s)

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Funding Scheme

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MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2019

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Coordinator

UNIVERSIDAD DE SANTIAGO DE COMPOSTELA
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 253 227,84
Address
COLEXIO DE SAN XEROME PRAZA DO OBRADOIRO S/N
15782 SANTIAGO DE COMPOSTELA
Spain

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Region
Noroeste Galicia A Coruña
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 253 227,84

Partners (1)

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