The research project focused on the design, synthesis, and biological evaluation of antigenic peptides targeted to the langerin receptor, with the aim of enhancing immune responses for various applications including skin-based vaccinations, cancer therapy, and prevention of graft rejection and autoimmune diseases. The project progressed through several work packages, each involving specific tasks.
Work Performed:
Design and Synthesis: Antigenic peptides and a glycomimetic targeting ligand were designed to interact with the langerin receptor. Peptides from Ovalbumin (OVA) were chosen as model antigens. The project also focused on creating a cleavable linker and the targeting ligand.
Synthesis Challenges: Challenges were faced during the synthesis of targeting glycopeptides due to coupling issues. Alternative strategies were explored to construct the glycopeptides.
Cell Culture Experiments: Cell culture experiments were conducted to study the interaction between liposomes carrying the targeting ligand and langerin-expressing cells. Successful antigen presentation onto MHC II complexes was observed, suggesting the potential of lipid-based nanoparticles to boost immune responses.
Main Results Achieved:
Peptides from OVA were successfully synthesized, though the targeting glycopeptides were not completed in the given time frame due to synthesis challenges.
Liposomes with the targeting ligand were found to interact with langerin-expressing cells, enhancing the presentation of antigenic peptides on MHC II complexes.
Final Period Overview:
Despite considerable effort, the synthesis of targeting glycopeptides was not fully accomplished within the project's timeline due to coupling issues. Alternative approaches were explored. The project duration was shortened due to logistical and budget adjustments.
Exploitation and Dissemination:
The researcher actively shared project progress and findings through various means, such as press releases, interviews, seminars, podcasts, and participation in Women in Science events. The project contributed to the researcher's skill development in immunology, drug delivery, and nanoparticle formulation, even though the desired glycopeptide constructs weren't achieved as planned.
