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Enzyme-conjugated DNA nanoswitches for biosensing applications

Descrizione del progetto

Nano-biosensori basati su enzimi per scopi diagnostici

Il progetto ENZYME-SWITCHES, finanziato dall’UE, è una proposta interdisciplinare che mira a sviluppare nuovi biosensori basati su enzimi per applicazioni diagnostiche. I ricercatori combineranno la specificità e la sensibilità elevate degli enzimi con la flessibilità e la versatilità programmabili dei nanodispositivi di DNA sintetici reattivi per creare enzimi coniugati con DNA come marcatori diagnostici sensibili. Il progetto coinvolge partner con esperienza in biologia molecolare, enzimologia, tecnologia dei biosensori, dispositivi di bioingegneria e biofisica, nonché specialisti in nanotecnologia del DNA, chimica analitica ed elettrochimica.

Obiettivo

WHY: Diagnostic markers detection plays a crucial role in the diagnosis of human disorders. Unfortunately, however, current methods routinely used for the detection of these biomarkers still require sophisticated equipment, washing intensive, reagent-intensive multi-step processes. In attempt to overcome the cited limitations, the development of platforms supporting the sensitive, specific, rapid and easy to use measurement for several diagnostic biomarkers, directly in real samples, is urgently needed.

WHAT: ENZYME-SWITCHES is a multidisciplinary and interdisciplinary programme with the principal aim to develop a new generation of enzyme-based biosensors that could prove transformative for diagnostic applications. To achieve this goal, I will join the high specificity and sensitivity of enzymes, and the high programmability and versatility of synthetic responsive DNA nanodevices to develop DNA-conjugated enzymes able to provide sensitive detection of a wide range of diagnostic markers.

HOW: This challenging objective will be reached thanks to the multidisciplinary and complementary approach of the outgoing and incoming research groups. I will join the expertise of Prof. Plaxco (outgoing supervisor) in molecular biology, enzymes, biosensor technology, bioengineering device and biophysic with that of Prof. Ricci (incoming supervisor) in the DNA-Nanotechnology field, analytical chemistry and electrochemistry to optimize the proposed new biosensors platform.

I have already a promising research profile demonstrated by a strong publication track-record, by several awards received and by fruitful international mobility. Thanks to this MSC Fellowship, I will further strengthen my CV and diversify my scientific knowledge acquiring cutting-edge competences as well as complementary skills, which will boost my scientific independence and my future career.

Campo scientifico (EuroSciVoc)

Parole chiave

Programma(i)

Argomento(i)

Meccanismo di finanziamento

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

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Invito a presentare proposte

(si apre in una nuova finestra) H2020-MSCA-IF-2019

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Coordinatore

UNIVERSITA DEGLI STUDI DI ROMA TOR VERGATA
Contributo netto dell'UE
€ 168 369,60
Indirizzo
VIA CRACOVIA 50
00133 Roma
Italia
Regione
Centro (IT) Lazio Roma
Tipo di attività
Higher or Secondary Education Establishments
Collegamenti
Costo totale
€ 168 369,60

Partner (1)