Descripción del proyecto
Secuenciación «in situ» para mapear las interacciones celulares en la mucosa intestinal
En condiciones naturales, el epitelio intestinal es una barrera física dinámica que actúa como primera línea de defensa inmunitaria. El daño a la monocapa de células del epitelio intestinal altera la homeostasis inmunitaria del intestino y conduce a enfermedades inflamatorias intestinales (EII), como la colitis ulcerosa y la enfermedad de Crohn. Dada la creciente incidencia de las EII en las sociedades modernas, los científicos del proyecto financiado con fondos europeos GUTMAPS tienen como objetivo investigar el tejido intestinal con una resolución sin precedentes. Para ello, realizarán una secuenciación «in situ» para perfilar células individuales, mapear interacciones entre células y ayudar a comprender mejor este órgano tanto en la salud como en la enfermedad.
Objetivo
The intestinal epithelium is the first line of defence of the mucosal immune system because it acts as a dynamic physical barrier segregating the luminal content from the underlying mucosal tissue. This barrier is mainly formed by a monolayer of specialised intestinal epithelial cells (IECs) that are crucial in maintaining intestinal homeostasis. Damage to this epithelial layer can increase intestinal permeability and lead to abnormalities in interactions between IECs, stromal cells and immune cells in the underlying lamina propria thereby disturbing the intestinal immune homeostasis, all of which are a hallmark of several intestinal disorders including inflammatory bowel diseases (IBD). Ulcerative colitis (UC) and Crohn’s disease (CD) are the two major forms of IBD, affecting an estimated 4 million people in the United States and Europe and have a rising incidence in the developing world. Recent single cell RNA seq (scRNAseq) studies of the intestine have allowed us to understand this organ in unprecedented detail, however, such studies still require the dissociation of tissue and loss of spatial resolution. With this project, I would like to take advantage of recent advances in in-situ sequencing to study intestinal tissue in toto and by combining this with the available scRNA-seq data, generate spatial maps of the intestine (GUTMAPS). The results obtained here will allow us to look at tissue composition and cell-cell interactions with unprecedented resolution in normal and diseased mucosa.
Ámbito científico
- natural sciencesearth and related environmental sciencesphysical geographycartography
- medical and health sciencesclinical medicinegastroenterologyinflammatory bowel disease
- medical and health sciencesbasic medicineimmunology
- natural sciencesbiological sciencesgeneticsRNA
- medical and health sciencesbasic medicinephysiologyhomeostasis
Palabras clave
Programa(s)
Régimen de financiación
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinador
3015 GD Rotterdam
Países Bajos