First use of probabilistic programming for hard problems in statistical phylogenetics

Projektinformationen

PhyPPL

ID Finanzhilfevereinbarung: 898120

DOI

10.3030/898120

Projekt abgeschlossen

EK-Unterschriftsdatum 8 April 2020

Startdatum 4 Mai 2020

Enddatum 24 August 2022

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten

€ 203 852,16

EU-Beitrag

€ 203 852,16

Koordiniert durch

NATURHISTORISKA RIKSMUSEET
Sweden

Periodic Reporting for period 1 - PhyPPL (First use of probabilistic programming for hard problems in statistical phylogenetics)

Berichtszeitraum: 2020-05-04 bis 2022-05-03

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

Phylogenetics is a scientific field that aims to reconstruct the evolutionary history of species and understand the factors that may have influenced it. By taking into account the evolutionary relationships between species, we can properly evaluate present and future-day biodiversity, especially in the face of challenges such as climate change. However, despite advances in mathematical modeling, the complexity of planetary interactions can make it challenging to develop accurate models.

The Marie Curie Action 898120 (PhyPPL) aimed to push the boundaries of what is possible in computational phylogenetics by adapting the method of universal probabilistic programming to the field. Through several stages of development, including proof of concept, implementation of adapted software, and practical application of the software, the ESR was able to develop a novel and previously un-attempted model with under 1,000 lines of code (anagenetic diversification rate shifts). This accomplishment is a significant achievement as comparable implementations in legacy frameworks often require 10x as much code.

The results of the study suggest that probabilistic programming is the best way to specify phylogenetic models, and the team's newly developed model provides a better explanation of the diversification history of birds. Specifically, the study found that many small shifts in the evolutionary rates along the branches of the evolutionary tree may explain diversification patterns better than burst-style changes in rates that had been hypothesized before. These findings have implications not only for the study of bird diversification but also for the broader field of evolutionary biology.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

During the first stage of the project, we investigated the possibility of encoding existing phylogenetic models as universal probabilistic programs to perform numerical experiments efficiently. Our investigation resulted in the publication of a concept paper titled "Universal probabilistic programming offers a powerful approach to statistical phylogenetics" in Communication Biology ([https://www.nature.com/articles/s42003-021-01753-7](https://www.nature.com/articles/s42003-021-01753-7)).

In the second stage of the project, we learned from the first stage and adapted universal probabilistic programming to our field. We created a novel compiler with a more efficient inference engine uniquely suited to the structure of the programs that we had analyzed before. This work resulted in the publication of the award-winning "Compiling Universal Probabilistic Programming Languages with Efficient Parallel Sequential Monte Carlo Inference" during the ESOP 2021 conference ([https://link.springer.com/chapter/10.1007/978-3-030-99336-8_2](https://link.springer.com/chapter/10.1007/978-3-030-99336-8_2)). Another paper of which the ESR is co-author and which deals with improvements on the inference engine in the new compiler is in the later stages of preparation.

Third phase: generic PPLs did not reflect the needs of evolutionary biologists for a straightforward modeling framework. Therefore, we set out to create an ergonomic syntax for our efficient compiler named TreePPL ([https://treeppl.org/](https://treeppl.org/)). The work has been presented during a workshop at KTH Sweden on December 14, 2023 (https://miking.org/workshop-2022) and an upcoming release note paper is in preparation, of which the ESR will be the first author.

In the fourth phase of the project, we developed a novel macro-evolutionary model called anagenetic diversification rate-shifts under geometric Brownian motion (AnaDS-BDD). We implemented this model in the underlying framework of TreePPL and found that it outperforms its peers. Our model indicates that the processes of speciation and extinction are mostly influenced by very small phenotypic changes along the evolutionary lineages of birds, rather than by large splitting events as previously thought. We presented these results as a poster at the European Congress of Evolutionary Biology ESEB 2022 ([https://github.com/phyppl/eseb-2022-poster](https://github.com/phyppl/eseb-2022-poster)). An academic article is in the final stages of drafting (first authorship of the ESR) and will be submitted for publication later in a few months.

As an additional side-output of the action during 2020, we used our tools to combat the Covid-19 pandemic. We developed an open-source tool to estimate the prevalence of Covid-19 cases using probabilistic programming called `prevestim` ([https://phyppl.github.io/prevestim/](https://phyppl.github.io/prevestim/)). The tool was used to create the protocol for a "Seroepidemiologic study to detect antibody-mediated immunity against SARS-CoV-2 among residents and healthcare workers in the city of Plovdiv, Bulgaria, weeks 21-24, 2020." We presented this analysis as a contribution at the leading European epidemiologic conference ESCAIDE.

Exploitation and Dissemination of Results: The key outcome of this project is the development of TreePPL, a powerful tool that leverages the latest advancements in probabilistic machine learning to create a modeling framework tailored to the needs of evolutionary biologists. TreePPL is not only being used for scientific purposes during the project, but also beyond its completion. The ESR is utilizing the tool in his new role at the École normale supérieure in Paris, and it is also being shared with other researchers in Stockholm.

In addition to scientific papers and lectures, the ESR has also disseminated awareness about probabilistic modeling through a popular science YouTube channel. In the hour-long video interview ([https://www.youtube.com/watch?v=V0vAMUuR0H0&t=2036s](https://www.youtube.com/watch?v=V0vAMUuR0H0&t=2036s)) he explains the concepts of probabilistic modeling in a clear and accessible way, making them accessible to a wider audience.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

Thanks to the PhyPPL project, the field of phylogenetics has made significant progress beyond the state of the art. The project has introduced a powerful new method, universal probabilistic programming, which is more expressive and more efficient than previously possible methods. The introduction of a novel programming language, TreePPL, has taken advantage of these two features. The potential impact of TreePPL is significant, and we expect it to be comparable to other widely used tools such as MrBayes and RevBayes, which have tens of thousands of academic citations.

As the project continues, we anticipate that TreePPL will be used to construct even more sophisticated and accurate evolutionary models. We also expect the development of new applications of the method, beyond phylogenetics, to other fields in biology, ecology, and epidemiology. The impact of TreePPL may extend beyond the academic community, with potential implications for industry and public policy.

Furthermore, the PhyPPL project has wider societal implications. The project's approach to modeling evolution is grounded in probability theory and machine learning, which is becoming increasingly relevant in today's data-driven society. The project's methods and tools may have implications for fields such as finance, engineering, and artificial intelligence, as well as for public policy and decision-making.

Illustration of the novel AnaDS-BDD model