ADDovenom: Novel Snakebite Therapy Platform of Unparalleled Efficacy, Safety and Affordability

Informazioni relative al progetto

ADDovenom

ID dell’accordo di sovvenzione: 899670

DOI

10.3030/899670

Progetto chiuso

Data della firma CE 26 Maggio 2020

Data di avvio 1 Ottobre 2020

Data di completamento 31 Marzo 2025

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 3 675 558,75

Contributo UE

€ 3 675 558,75

Coordinato da

UNIVERSITY OF BRISTOL
United Kingdom

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (15)

List of protein / peptide components for 8 African snake venoms and their relative abundance identified by MS

List of protein peptide components for 8 African snake venoms and their relative abundance identified by MS

White paper 1
List of identified toxins and their predicted pharmacological activity in the venoms (8 snakes)
List of sequences, PTMs, disulphide bridges and isoelectric points of the venom components (2 snakes)

List of sequences PTMs disulphide bridges and isoelectric points of the venom components 2 snakes

Pharmokinetic characterisation of ADDomer/ADDobodies in mice
Validated protocols for the production of ADDobodies in high-throughput and milligram amounts

Validated protocols for the production of ADDobodies in highthroughput and milligram amounts

Determination of venom toxicity (LD50) for each snake species

Determination of venom toxicity LD50 for each snake species

In process assays for ADDomers
Demonstration of safety of ADDomer/ADDobodies in mice
List of sequences, PTMs, disulphide bridges and isoelectric points of the venom components (8 snakes)
List of protein / peptide components for 2 African snake venoms and their relative abundance identified by MS

List of protein peptide components for 2 African snake venoms and their relative abundance identified by MS

Validated protocols for the production of toxins/ epitope strings

Validated protocols for the production of toxins epitope strings

In vitro functional ability of ADDomers/ADDobodies to neutralise toxin-function
List of identified toxins and their predicted pharmacological activity in the venoms (2 snakes)

List of identified toxins and their predicted pharmacological activity in the venoms 2 snakes

Determination of ADDomers/ADDobodies to neutralize venom-induced lethality using in vivo ED50 assays

Websites, patent fillings, videos etc. (2)

First version of the website
Toxin database

Open Research Data Pilot (1)

Data management plan

Pubblicazioni

Peer reviewed articles (9)

Virus-like particles displaying conserved toxin epitopes stimulate polyspecific, murine antibody responses capable of snake venom recognition

Autori: Stefanie K. Menzies, Charlotte A. Dawson, Edouard Crittenden, Rebecca J. Edge, Steven R. Hall, Jaffer Alsolaiss, Mark C. Wilkinson, Nicholas R. Casewell, Robert A. Harrison, Stuart Ainsworth
Pubblicato in: Scientific Reports, Numero 12, 2022, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-022-13376-x

Dual Proteomics Strategies to Dissect and Quantify the Components of Nine Medically Important African Snake Venoms

Autori: Damien Redureau, Fernanda Gobbi Amorim, Thomas Crasset, Imre Berger, Christiane Schaffitzel, Stefanie Kate Menzies, Nicholas R. Casewell, Loïc Quinton
Pubblicato in: Toxins, Numero 17, 2025, Pagina/e 243, ISSN 2072-6651
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins17050243

Virus-like particles displaying conserved toxin epitopes stimulate polyspecific, murine antibody responses capable of snake venom recognition

Autori: Stefanie K. Menzies; Charlotte A. Dawson; Edouard Crittenden; Rebecca J. Edge; Steven R. Hall; Jaffer Alsolaiss; Mark C. Wilkinson; Nicholas R. Casewell; Robert A. Harrison; Stuart Ainsworth
Pubblicato in: Scientific Reports, 2022, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41598-022-13376-x

De novo designed proteins neutralize lethal snake venom toxins

Autori: Susana Vázquez Torres, Melisa Benard Valle, Stephen P. Mackessy, Stefanie K. Menzies, Nicholas R. Casewell, Shirin Ahmadi, Nick J. Burlet, Edin Muratspahić, Isaac Sappington, Max D. Overath, Esperanza Rivera-de-Torre, Jann Ledergerber, Andreas H. Laustsen, Kim Boddum, Asim K. Bera, Alex Kang, Evans Brackenbrough, Iara A. Cardoso, Edouard P. Crittenden, Rebecca J. Edge, Justin Decarreau, Robert J
Pubblicato in: Nature, Numero 639, 2025, Pagina/e 225-231, ISSN 0028-0836
Editore: Nature Publishing Group
DOI: 10.1038/s41586-024-08393-x

Engineering the ADDobody protein scaffold for generation of high-avidity ADDomer super-binders

Autori: Dora Buzas, Huan Sun, Christine Toelzer, Sathish K. N. Yadav, Ufuk Borucu, Gunjan Gautam, Kapil Gupta, Josh Bufton, Julien Capin, Richard B. Sessions, Frederic Garzoni, Imre Berger, Christiane Schaffitzel
Pubblicato in: Structure, 2023, ISSN 0969-2126
Editore: Cell Press
DOI: 10.1101/2023.09.09.556966

Next-Generation Sequencing for Venomics: Application of Multi-Enzymatic Limited Digestion for Inventorying the Snake Venom Arsenal

Autori: Fernanda Gobbi Amorim, Damien Redureau, Thomas Crasset, Lou Freuville, Dominique Baiwir, Stefanie K. Menzies, Nicholas R. Casewell, Loïc Quinton
Pubblicato in: Toxins, Numero 15(6), 2023, Pagina/e 357, ISSN 2072-6651
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins15060357

Preclinical evaluation of the neutralising efficacy of three antivenoms against the venoms of the recently taxonomically partitioned E. ocellatus and E. romani

Autori: Rebecca J. Edge, Amy E. Marriott, Molly Keen, Tiffany Xie, Edouard P. Crittenden, Charlotte A. Dawson, Mark C. Wilkinson, Wolfgang Wüster, Nicholas R. Casewell, Stuart Ainsworth, Stefanie K. Menzies
Pubblicato in: PLOS Neglected Tropical Diseases, Numero 19, 2025, Pagina/e e0013371, ISSN 1935-2735
Editore: Public Library of Science (PLoS)
DOI: 10.1371/journal.pntd.0013371

In vitro generated antibodies guide thermostable ADDomer nanoparticle design for nasal vaccination and passive immunization against SARS-CoV-2

Autori: Dora Buzas, Adrian H Bunzel, Oskar Staufer, Emily J Milodowski, Grace L Edmunds, Joshua C Bufton, Beatriz V Vidana Mateo, Sathish K N Yadav, Kapil Gupta, Charlotte Fletcher, Maia K Williamson, Alexandra Harrison, Ufuk Borucu, Julien Capin, Ore Francis, Georgia Balchin, Sophie Hall, Mirella V Vega, Fabien Durbesson, Srikanth Lingappa, Renaud Vincentelli, Joe Roe, Linda Wooldridge, Rachel Burt, Ross
Pubblicato in: Antibody Therapeutics, Numero tbad024, 2023, ISSN 2516-4236
Editore: Oxford Academic
DOI: 10.1093/abt/tbad024

ADDovenom: Thermostable Protein-Based ADDomer Nanoparticles as New Therapeutics for Snakebite Envenoming

Autori: Stefanie K. Menzies, Raquel A. Garcia, Fernanda Gobbi Amorim, Iara Aimê Cardoso,Camille Abada, Thomas Crasset, Rebecca J. Edge, Sophie Hall, Damien Redureau, Richard Stenner, Johara Stringari, António Roldão, Paula M.vAlves, Robert A. Harrison, Renaud Vincentelli, Huan Sun, Imre Berger, Loïc Quinton, Nicholas R. Casewell, Christiane Schaffitzel
Pubblicato in: Toxins, Numero 15(12), 2023, Pagina/e 673, ISSN 2072-6651
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/toxins15120673