Descripción del proyecto
Diseño y creación de motores proteicos autónomos
El fin último del proyecto financiado con fondos europeos ArtMotor es diseñar y construir desde la base motores proteicos sintéticos y funcionales que sean capaces de transportar y transducir energía. El proyecto aprovechará la experiencia en diseño de proteínas por ordenador, biología estructural y molecular, y la utilización de módulos proteicos no motores ya existentes con una función molecular conocida. Los investigadores construirán motores proteicos relativamente sencillos que requieren un control externo a la vez que tratan de crear un motor proteico autónomo capaz de desplazarse por una vía. La creación de motores proteicos autónomos con una amplia variedad de propiedades, funcionalidades y características de rendimiento podría posibilitar la transducción de la energía química en trabajo mecánico de manera más eficiente que los motores de combustión fabricados por humanos.
Objetivo
Molecular motors and machines are essential for all cellular processes that together enable life. Built from proteins, with a wide range of properties, functionalities and performance characteristics, biological motors perform complex tasks and can transduce chemical energy into mechanical work more efficiently than human-made combustion engines. Sophisticated studies of biological protein motors have led to much structural and biophysical information and the development of models for motor function.
However, from the study of highly evolved, biological motors it remains difficult to discern detailed mechanisms, for example about the relative role of different force generation mechanisms, or how information is communicated across a protein to achieve the necessary coordination. A promising, complementary approach to answering these questions is to build synthetic protein motors from the bottom up. Indeed, much effort has been invested in functional protein design, but so far, the ‘holy grail’ of designing and building a functional synthetic protein motor has not been realized.
The purpose of ArtMotor is to design and build functional, synthetic protein motors capable of moving and transducing energy, based on existing, non-motor protein modules of known molecular function. Harnessing the synergy of expertise in computational protein design, structural and molecular biology, and single-molecule detection, we will use a two-pronged approach to (a) construct relatively simple protein motors that will require external control, while (b) construct, step by step, an autonomous protein motor capable of moving along a track. Such a functional, synthetic protein will constitute a ground-breaking advance in synthetic biology, physics and engineering. In addition to gaining new insights into mechanisms of energy transduction in proteins, we will also inspire other, complex protein designs that may lead to advances in fields from enzyme design to nano-engineering.
Ámbito científico
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Programa(s)
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Régimen de financiación
ERC-SyG - Synergy grantInstitución de acogida
22100 Lund
Suecia