Descrizione del progetto
Nanoparticelle con una superficie biogenica
Le vescicole extracellulari sono le navette universali di comunicazione intercellulare, trasferendo lipidi, proteine e acidi nucleici, mediando i processi fisiologici e trasmettendo varie malattie, tra cui il cancro e le infezioni. L’obiettivo principale del progetto BOW, finanziato dall’UE, è studiare e sviluppare la tecnologia in grado di fornire le capacità della superficie biologica delle vescicole extracellulari, in termini di precisione, circolazione e di targeting, ai nanodispositivi superparamagnetici, rivestendoli con una membrana di vescicole extracellulari a strato singolo o multistrato. Questa tecnologia è volta a far avanzare nanodispositivi e nanomateriali impiantabili verso una produzione sostenibile e nella traduzione clinica, dimostrando la possibilità di ricapitolare le funzioni biomimetiche su qualsiasi nanodispositivo sintetico.
Obiettivo
Extracellular vesicle (EVs) nanoparticles are the universal agents of intercellular and inter-organismal communication “made by cells for cells” to shuttle lipids, proteins and nucleic acids, EVs mediate physiological processes and help to spread various diseases, including cancer and infections. Their innate navigation performances take origin in the unique structure and composition of their membrane (which is to date inaccessible to synthetic mimics). The main goal of the BOW project is to explore and consolidate the technology able to impart biological surface precision, circulation and targeting abilities of EVs to superparamagnetic nanodevices (Magnetic Bead Devices, MBDs) by “dressing” them with a single- or multi-layer “wetsuit” of EV membrane “fabric”. This will proof and set a general, viable paradigm to recapitulate key biomimetic functions – including camouflage to the immune system and organ site/tumor targeting – to any synthetic nanodevice, while being disruptive as a first example of biogenic nanotechnology. If successful, such a non-incremental technology will promote the progress of implantable nanodevices and nanomaterials towards sustainable production and clinical translation, contributing to strengthen and keep in the lead position European biotechnology and impacting life quality for people. Major objectives include: (i) production high-grade EVs with biomimetic and organotropic functions, (ii) synthesis and functionalization of MBDs, (iii) engineering a microfluidic device for streamlined fabrication of EV membrane coated MBDs (evMBDs) (iv) evaluation of evMBD biological performances and nanotoxiciy in-vitro, ex-vivo and in-vivo. BOW will be made possible thanks to a balanced ecology-biology-biophysics-chemistry-engineering matrix, of well-established and internationally recognized academics (7), high biotech SMEs (3), plus 1 innovation consulting, contributing to strengthen European pool of expertise and biotechnology innovation eco-system.
Campo scientifico
- natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acids
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteins
- natural sciencesbiological sciencescell biology
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- social scienceseconomics and businesseconomicssustainable economy
Parole chiave
Programma(i)
Invito a presentare proposte
Vedi altri progetti per questo bandoBando secondario
H2020-EIC-FETPROACT-2019
Meccanismo di finanziamento
RIA - Research and Innovation actionCoordinatore
50019 Firenze
Italia