Biogenic Organotropic Wetsuits

Leistungen

Promotional material

First version of the promotional material newsletters flyer videos video news release

Second promotional material

Second version of the promotional material newsletters flyer videos video news release

Website, social network profiles and CMS guide

Project web site and social network profiles CMS guide for all partners

First report on C&D activities

First report on CD activities

Functionalization of EVs

Setting up and validation of strategy to attach different proteinsantibodies or sugars to target cells

SOPs for synthesis of single-core magnetite MBDs

Standard Operating Procedure SOPs on single core magnetite MBDs SPIONS synthesis

Project management handbook

CSGI will produce a project handbook for project management and quality assurance It will define the guidelines for reporting and internal procedures

SOPs for synthesis of multi-core magnetite MBDs

SOPs on multicore magnetite MBDs

SOPs for derivatization of multi-core magnetite MBDs

SOPs on derivatized MBDs SPMbeads functionalization

SOPs for derivatization of single-core magnetite MBDs

SOPs on derivatized MBDs SPIONS functionalization

Project Exploitation and Dissemination Plan

First version of the project Exploitation and Dissemination Plan PEDR

Updated Project Exploitation and Dissemination Plan

Updated version of the project Exploitation and Dissemination Plan PEDR

Production of microalgal-derived EVs

We will generate two SOPs related to 1 the cultivation of a microalgal strains suitable for the production of EVs 2 the production and separation of microalgal EVs

Characterization of EVs

A quality check of EDTMSC and microalgal EVs will be generated it will include size distribution density morphology concentration stability in biological fluids and EVaggregate ratio purity

Data Management Plan

A Data Management Plan will be elaborated considering the H2020 open access rules and research data management to ensure that outputs are findable accessible interoperable and reusable to scientific publications rules

Veröffentlichungen

Opportunities and Pitfalls of Fluorescent Labeling Methodologies for Extracellular Vesicle Profiling on High-Resolution Single-Particle Platforms

Autoren: Fortunato D, Mladenović D, Criscuoli M, Loria F, Veiman K-L, Zocco D, Koort K, Zarovni N
Veröffentlicht in: International Journal of Molecular Sciences, 2021, ISSN 1422-0067
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms221910510

Selective isolation of extracellular vesicles from minimally processed human plasma as a translational strategy for liquid biopsies

Autoren: Diogo Fortunato; Stavros Giannoukakos; Ana Giménez-Capitán; Michael Hackenberg; Miguel A. Molina-Vila; Nataša Zarovni
Veröffentlicht in: Crossref, Ausgabe 17417007, 2022, ISSN 1741-7007
Herausgeber: BioMed Central
DOI: 10.1186/s40364-022-00404-1

Particle profiling of EV-lipoprotein mixtures by AFMnanomechanical imaging

Autoren: Andrea Ridolfi, Laura Conti, Marco Brucale, Roberto Frigerio, Jacopo Cardellini, Angelo Musicò, Miriam Romano, Andrea Zendrini, Laura Polito, Greta Bergamaschi, Alessandro Gori, Costanza Montis, Stefano Panella, Lucio Barile, Debora Berti, Annalisa Radeghieri, Paolo Bergese, Marina Cretich, Francesco Valle
Veröffentlicht in: Journal of Extracellular Vesicles, Ausgabe Volume 12, Ausgabe 10, 2023, Seite(n) n/a, ISSN 2001-3078
Herausgeber: Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicle
DOI: 10.1002/jev2.12349

Membrane Phase Drives the Assembly of Gold Nanoparticles on Biomimetic Lipid Bilayers

Autoren: Jacopo Cardellini, Lucrezia Caselli, Enrico Lavagna, Sebastian Salassi, Heinz Amenitsch, Martino Calamai, Costanza Montis, Giulia Rossi, and Debora Berti*
Veröffentlicht in: The Journal of Physical Chemistry C, Ausgabe 2022 126 (9), 2022, Seite(n) 4483–4494, ISSN 1932-7447
Herausgeber: American Chemical Society
DOI: 10.1021/acs.jpcc.1c08914

Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters

Autoren: García Acevedo, Pelayo; González Gómez Manuel Antonio; Arnosa Prieto Ángela; de Castro Alves Lisandra; Seco Gudiña Román; Piñeiro Yolanda; Rivas José
Veröffentlicht in: Magnetochemistry, Ausgabe Volume 8; Ausgabe 8, 2022, Seite(n) 83, ISSN 2312-7481
Herausgeber: MDPI
DOI: 10.3390/magnetochemistry8080083

Nanoscale structural and mechanical characterization of thin bicontinuous cubic phase lipid films

Autoren: Andrea Ridolfi, Ben Humphreys, Lucrezia Caselli, Costanza Montis, Tommy Nylander, Debora Berti, Marco Brucale, Francesco Valle
Veröffentlicht in: Colloids and Surfaces B: Biointerfaces, Ausgabe Volume 210, February 2022, 2022, Seite(n) 112231, ISSN 0927-7765
Herausgeber: Elsevier BV
DOI: 10.1016/j.colsurfb.2021.112231

Interaction of Metallic Nanoparticles With Biomimetic Lipid Liquid Crystalline Cubic Interfaces

Autoren: Jacopo Cardellini, Costanza Montis, Francesco Barbero, Ilaria De Santis, Lucrezia Caselli and Debora Berti
Veröffentlicht in: Frontiers in bioengineering and biotechnology., Ausgabe 22964185, 2022, ISSN 2296-4185
Herausgeber: Frontiers
DOI: 10.3389/fbioe.2022.848687

Significant Surface Spin Effects and Exchange Bias in Iron Oxide-Based Hollow Magnetic Nanoparticles

Autoren: Pelayo García Acevedo; Manuel A. González Gómez; Ángela Arnosa Prieto; Jose S. Garitaonandia; Yolanda Piñeiro; José Rivas
Veröffentlicht in: Nanomaterials, Vol 12, Iss 456, p 456 (2022), Ausgabe 1, 2021, ISSN 2079-4991
Herausgeber: Nanomaterials
DOI: 10.3390/nano12030456

Two Dimensional-Difference in Gel Electrophoresis (2D-DIGE) Proteomic Approach for the Identification of Biomarkers in Endometrial Cancer Serum

Autoren: Blendi Ura, Stefania Biffi, Lorenzo Monasta, Giorgio Arrigoni, Ilaria Battisti, Giovanni Di Lorenzo, Federico Romano, Michelangelo Aloisio, Fulvio Celsi, Riccardo Addobbati, Francesco Valle, Enrico Rampazzo, Marco Brucale, Andrea Ridolfi, Danilo Licastro, Giuseppe Ricci
Veröffentlicht in: Cancers, Ausgabe 13/14, 2021, Seite(n) 3639, ISSN 2072-6694
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers13143639

Versatile magnetic confguration for the control and manipulation of superparamagnetic nanoparticles

Autoren: Surpi Alessandro; Shelyakova Tatiana; Murgia Mauro; Rivas José; Piñeiro Yolanda; Greco Pierpaolo; Fini Milena; Dediu Valentin Alek
Veröffentlicht in: Scientific Reports, 2023, ISSN 2045-2322
Herausgeber: Nature Publishing Group
DOI: 10.1038/s41598-023-32299-9

Magnetic separation and concentration of Aβ 1–42 molecules dispersed at the threshold concentration for Alzheimer’s disease diagnosis in clinically-relevant volumes of sample

Autoren: Alessandro Surpi, Mauro Murgia, Sonia López-Amoedo, Manuel A. González-Gómez, Yolanda Piñeiro, José Rivas, Valeria Perugini, Matteo Santin, Tomás Sobrino, Pierpaolo Greco, Francisco Campos, Valentin Alek Dediu
Veröffentlicht in: Journal of Nanobiotechnology, Ausgabe 21, Article number: 329 (2023), 2023, ISSN 1477-3155
Herausgeber: BioMed Central
DOI: 10.1186/s12951-023-02095-8

Biocompatible magnetic gelatin nanoparticles with enhanced MRI contrast performance prepared by single-step desolvation method

Autoren: C Teijeiro-Valiño, M A González Gómez, S Yáñez, P García Acevedo, A Arnosa Prieto, S Belderbos, W Gsell, U Himmelreich, Y Piñeiro, J Rivas
Veröffentlicht in: Nano Express, Ausgabe 2/2, 2021, Seite(n) 020011, ISSN 2632-959X
Herausgeber: Nano Express
DOI: 10.1088/2632-959x/abf58e

Impact of the flame retardant 2,2’4,4’-tetrabromodiphenyl ether (PBDE-47) in THP-1 macrophage-like cell function via small extracellular vesicles

Autoren: Valeria Longo, Noemi Aloi, Alessandra Longo, Giorgia Adamo, Antonella Bongiovanni and Paolo Colombo
Veröffentlicht in: Front. Immunol, 2022, ISSN 1664-3224
Herausgeber: Frontiers
DOI: 10.3389/fimmu.2022.1069207

Role of Dipolar Interactions on the Determination of the Effective Magnetic Anisotropy in Iron Oxide Nanoparticles

Autoren: Pelayo García-Acevedo, Manuel A. González-Gómez, Ángela Arnosa-Prieto, Lisandra de Castro-Alves, Yolanda Piñeiro, and José Rivas
Veröffentlicht in: Advanced Sciences, Ausgabe Volume 10, Ausgabe 5, February 14, 2023, 2023, Seite(n) 2203397, ISSN 2198-3844
Herausgeber: Wiley-VCH GmbH
DOI: 10.1002/advs.202203397

Multiparametric Orthogonal Characterization of Extracellular Vesicles by Liquid Chromatography Combined with In-Line Light Scattering and Fluorescence Detection

Autoren: Karl Normak, Marcell Papp, Michael Ullmann, Carolina Paganini, Mauro Manno, Antonella Bongiovanni, Paolo Bergese and Paolo Arosio
Veröffentlicht in: Analytical Chemistry, Ausgabe Volume 95, Ausgabe 33, August 22, 2023, 2023, Seite(n) 12195-12574, ISSN 0003-2700
Herausgeber: American Chemical Society
DOI: 10.1021/acs.analchem.3c02108

High-Yield Separation of Extracellular Vesicles Using Programmable Zwitterionic Coacervates

Autoren: Carolina Paganini, Umberto Capasso Palmiero,* Sabrina Picciotto, Alessandro Molinelli, Ilaria Porello, Giorgia Adamo, Mauro Manno, Antonella Bongiovanni, and Paolo Arosio
Veröffentlicht in: Small, Ausgabe 16136810, 2022, ISSN 1613-6810
Herausgeber: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202204736

Synthesis and Characterization of a Biocompatible Nanoplatform Based on Silica-Embedded SPIONs Functionalized with Polydopamine

Autoren: Miriam Romano, Manuel Antonio González Gómez, Pamela Santonicola, Noemi Aloi, Svenja Offer, Jana Pantzke, Samuele Raccosta, Valeria Longo, Alessandro Surpi, Silvia Alacqua, Giuseppina Zampi, Valentin Alek Dediu, Bernhard Michalke, Ralf Zimmerman, Mauro Manno, Yolanda Piñeiro, Paolo Colombo, Elia Di Schiavi, José Rivas, Paolo Bergese, and Sebastiano Di Bucchianico
Veröffentlicht in: ACS Biomaterials Science & Engineering, 2023, ISSN 2373-9878
Herausgeber: American Chemical Society
DOI: 10.1021/acsbiomaterials.2c00946

Extracellular Vesicles From Microalgae: Uptake Studies in Human Cells and Caenorhabditis elegans

Autoren: Picciotto; Santonicola; Paterna; Rao; Raccosta; Romancino; Noto; Touzet; Manno; Di Schiavi; Bongiovanni; Adamo
Veröffentlicht in: Frontiers, Ausgabe 1, 2022, ISSN 2296-4185
Herausgeber: Frontiers
DOI: 10.3389/fbioe.2022.830189

An alternative in vitro model considering cell-cell interactions in fiber-induced pulmonary fibrosis

Autoren: Jana Pantzke and Svenja Offer and Elias J. Zimmermann and Evelyn Kuhn and Thorsten Streibel and Sebastian Oeder and Sebastiano Di Bucchianico and Ralf Zimmermann
Veröffentlicht in: Toxicology Mechanisms and Methods, 2022, ISSN 1537-6516
Herausgeber: Taylor & Francis
DOI: 10.1080/15376516.2022.2156008

Fluorescent Magnetic Mesoporous Nanoprobes for Biotechnological Enhancement Procedures in Gene Therapy

Autoren: González-Gómez MA, Seco-Gudiña R, García-Acevedo P, Arnosa-Prieto Á, de Castro-Alves L, Piñeiro Y, Rivas J.
Veröffentlicht in: Magnetochemistry, 2023, ISSN 2312-7481
Herausgeber: Magnetochemistry
DOI: 10.3390/magnetochemistry9030067

Probing the coverage of nanoparticles by biomimetic membranes through nanoplasmonics

Autoren: Cardellini, Jacopo; Ridolfi, Andrea; Donati, Melissa; Giampietro, Valentina; Severi, Mirko; Brucale, Marco; Valle, Francesco; Bergese, Paolo; Montis, Costanza; Caselli, Lucrezia; Berti, Debora
Veröffentlicht in: Cardellini , J , Ridolfi , A , Donati , M , Giampietro , V , Severi , M , Brucale , M , Valle , F , Bergese , P , Montis , C , Caselli , L & Berti , D 2023 , ' Probing the coverage of nanoparticles by biomimetic membranes through nanoplasmonics ' , Journal of Colloid and Interface Science , vol. 640 , pp. 100-109 . https://doi.org/10.1016/j.jcis.2023.02.073, Ausgabe 1, 2023, Seite(n) Pages 100-109, ISSN 0021-9797
Herausgeber: Academic Press
DOI: 10.1016/j.jcis.2023.02.073

Malignant‐ascites‐derived small extracellular vesicles in advanced ovarian cancer patients: insights into the dynamics of the extracellular matrix

Autoren: Barbara Bortot, Maura Apollonio, Enrico Rampazzo, Francesco Valle, Marco Brucale, Andrea Ridolfi, Blendi Ura, Riccardo Addobbati, Giovanni Di Lorenzo, Federico Romano, Francesca Buonomo, Chiara Ripepi, Giuseppe Ricci, Stefania Biffi
Veröffentlicht in: Molecular Oncology, Ausgabe Volume15, Ausgabe12 December 2021, 2021, Seite(n) Pages 3596-3614, ISSN 1574-7891
Herausgeber: Elsevier BV
DOI: 10.1002/1878-0261.13110

Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop

Autoren: Lucia Paolini; Marta Monguió‐Tortajada; Marta Costa; Fabio Antenucci; Mario Barilani; Marta Clos‐Sansalvador; André Cronemberger Andrade; Tom A. P. Driedonks; Sara Giancaterino; Stephanie M. Kronstadt; Rachel R. Mizenko; Muhammad Nawaz; Xabier Osteikoetxea; Carla Pereira; Surya Shrivastava; Anders Toftegaard Boysen; Simonides Immanuel van de Wakker; Martijn J. C. van Herwijnen; Xiaoqin Wang;
Veröffentlicht in: Paolini , L , Monguió-Tortajada , M , Costa , M , Antenucci , F , Barilani , M , Clos-Sansalvador , M , Andrade , A C , Driedonks , T A P , Giancaterino , S , Kronstadt , S M , Mizenko , R R , Nawaz , M , Osteikoetxea , X , Pereira , C , Shrivastava , S , Boysen , A T , van de Wakker , S I , van Herwijnen , M J C , Wang , X , Watson , D C , Gimona , M , Kaparakis-Liaskos , M , Konstantinov , K ,, Ausgabe 16, 2022, ISSN 2768-2811
Herausgeber: Journal of Extracellular Biology
DOI: 10.1002/jex2.63

Effect of glycerol and H3PO4 on the bioactivity and degradability of rod-like SBA-15 particles with active surface for bone tissue engineering applications

Autoren: Zulema Vargas-Osorio, Artem Klotschan, Marcela Arango-Ospina, Yolanda Piñeiro, Liliana Liverani, José Rivas, Martin Michálek, Dušan Galusek, Aldo R.Boccaccini
Veröffentlicht in: Microporous and Mesoporous Materials, 2021, ISSN 1387-1811
Herausgeber: Elsevier BV
DOI: 10.1016/j.micromeso.2021.111543

Isolation of Extracellular Vesicles From Microalgae: A Renewable and Scalable Bioprocess

Autoren: Angela Paterna; Estella Rao; Giorgia Adamo; Samuele Raccosta; Sabrina Picciotto; Daniele Romancino; Rosina Noto; Nicolas Touzet; Antonella Bongiovanni; Mauro Manno
Veröffentlicht in: Crossref, Ausgabe 1, 2022, ISSN 2296-4185
Herausgeber: Frontiers
DOI: 10.3389/fbioe.2022.836747

On the interaction and nanoplasmonics of gold nanoparticles and lipoproteins

Autoren: Andrea Zendrini, Jacopo Cardellini, Roberto Frigerio, Marianna Bertoni, Debora Berti, Paolo Bergese
Veröffentlicht in: JCIS Open, Special issue for 11th Colloids Conference, Ausgabe Volume 11, October 2023, Article 100088, 2023, ISSN 2666-934X
Herausgeber: Elsevier
DOI: 10.1016/j.jciso.2023.100088

The biomolecular corona of extracellular nanoparticles holds new promises for advancing clinical molecular diagnostics

Autoren: Annalisa Radeghieri and Paolo Bergese
Veröffentlicht in: Expert Review of Molecular Diagnostics, 2023, ISSN 1744-8352
Herausgeber: Taylor & Francis
DOI: 10.1080/14737159.2023.2215927

Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols towards Downstream Isolation and Analysis

Autoren: Roberto Frigerio, Angelo Musicò, Marco Brucale, Andrea Ridolfi, Silvia Galbiati, Riccardo Vago, Greta Bergamaschi, Anna Maria Ferretti, Marcella Chiari, Francesco Valle, Alessandro Gori, Marina Cretich
Veröffentlicht in: Cells, Ausgabe 10/3, 2021, Seite(n) 544, ISSN 2073-4409
Herausgeber: Cells
DOI: 10.3390/cells10030544

Stiffness of Fluid and Gel Phase Lipid Nanovesicles: Weighting the Contributions of Membrane Bending Modulus and Luminal Pressurization

Autoren: Andrea Ridolfi, Lucrezia Caselli, Matteo Baldoni, Costanza Montis, Francesco Mercuri, Debora Berti, Francesco Valle, Marco Brucale
Veröffentlicht in: Langmuir, 2021, ISSN 0743-7463
Herausgeber: American Chemical Society
DOI: 10.1021/acs.langmuir.1c01660

Surface functionalization of extracellular vesicle nanoparticles with antibodies: a first study on the protein corona “variable”

Autoren: Angelo Musicò, Rossella Zenatelli, Miriam Romano, Andrea Zendrini, Silvia Alacqua, Selene Tassoni, Lucia Paolini, Chiara Urbinati, Marco Rusnati, Paolo Bergese, Giuseppe Pomarico, and Annalisa Radeghieri
Veröffentlicht in: Nanoscale Advances, 5, Ausgabe Ausgabe 18, 2023, 2023, Seite(n) 4703-4717, ISSN 2516-0230
Herausgeber: ROYAL SOCIETY OF CHEMISTRY
DOI: 10.1039/d3na00280b

A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles

Autoren: Lucrezia Caselli, Andrea Ridolfi, Jacopo Cardellini, Lewis Sharpnack, Lucia Paolini, Marco Brucale, Francesco Valle, Costanza Montis, Paolo Bergese, Debora Berti
Veröffentlicht in: Nanoscale Horizons, Ausgabe 6/7, 2021, Seite(n) 543-550, ISSN 2055-6756
Herausgeber: Nanoscale Horizons
DOI: 10.1039/d1nh00012h

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