Descrizione del progetto
Un complesso sistema organo su chip per la ricerca biomedica
La tecnologia degli organoidi ha raggiunto un punto di svolta nel campo della biomedicina, con un potenziale elevato nella patogenesi virale e nella ricerca antivirale. Il progetto GUTVIBRATIONS, finanziato dall’UE, abbinerà l’esperienza maturata in settori quali microfabbricazione, tecnologia degli organoidi, virologia, immunologia e bioingegneria al fine di realizzare un sistema organo su chip come modello modulare multiorgano dell’asse intestino-cervello umano. Questo sistema sarà realizzato abbinando la tecnologia dei substrati stampati in 3D con organoidi di intestino e cervello umani. Il sistema includerà uno strato epiteliale dell’intestino, delle cellule immunitarie, una barriera emato-encefalica e un organoide cerebrale, collegati in una combinazione verticale. Il substrato della matrice extracellulare denominato Biosilk, una proteina ricombinante della seta di ragno, produrrà una nicchia microambientale analoga a quella in vivo. Il progetto si propone di valutare la commercializzazione del sistema attraverso un’analisi di mercato e un piano aziendale.
Obiettivo
The battle against infectious diseases is hampered by lack of therapeutic innovations due to poor understanding of disease outcome in humans. Organoid technology is a major breakthrough for medical research that has traditionally relied heavily on animal models. In our current OrganoVIR consortium, we are at the forefront in establishing human organoids as superior models for viral pathogenesis and antiviral research. The next critical step is to integrate organoid models to create a complex multi-organ ex vivo model that better mimics the human physiology. This raises several challenges such as incorporation of various cell types, medium incompatibility, validation, high throughput, and robust ways to connect mature organoid models. At the same time, the model has to be accessible, user friendly, and affordable to all end users. Therefore, this proposed GUTVIBRATIONS consortium will leverage its expertise in microfabrication, organoid technology, virology, immunology, bioengineering, and materials science to deliver an enabling organ-on-chip system.
As a demonstrator, a modular human gut-brain axis multi-organ model will be achieved for studying viral disease and treatment. This model will be built by combining an open source 3D printed scaffold technology with human gut and brain organoid models. The deliverables will include a complex gut epithelial layer, immune cell layer, blood-brain layer and brain organoid; all individually developed and subsequently connected by vertical stacking. Our unique modular approach where individual components are separately developed and validated minimizes compatibility issues. The individual models and the multi-organ model will be used for studying three different clinically relevant viral infections with high global burden and will be validated against existing clinical data to bring the system to TRL5. Routes towards commercialization will also be assessed by performing a market analysis and developing a business plan.
Campo scientifico
- natural sciencesbiological sciencesmicrobiologyvirology
- medical and health scienceshealth sciencesinfectious diseases
- medical and health sciencesbasic medicineimmunology
- medical and health sciencesbasic medicinephysiology
- engineering and technologyother engineering and technologiesmicrotechnologyorgan on a chip
Parole chiave
Programma(i)
Invito a presentare proposte
Vedi altri progetti per questo bandoBando secondario
H2020-NMBP-TR-IND-2020-twostage
Meccanismo di finanziamento
RIA - Research and Innovation actionCoordinatore
1081 HV Amsterdam
Paesi Bassi