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Ultrafast Raman Technologies for Protein Identification and Sequencing

Objective

The human proteome is the whole set of protein that a human can potentially express. Most of the human proteome is known. However the proteome, being the set of proteins potentially expressed, does not give information regarding the protein really expressed in a specific person or a patient. The possibility of accessing to this fundamental information through a cost and time effective technique will revolutionize our ability to prevent, diagnose and treat most of the human diseases.
This is the commitment of ProID that aims to provide a technological platform able to record single protein Raman spectra with single amino-acid resolution. Namely, by reading the sequence of selected amino-acids along the protein chain, the platform will identify the corresponding protein. To reach this goal we want to combine advanced nanofabrications of plasmonic nanopores with ultrafast time resolved photon detectors and machine learning algorithms. More in details: i) plasmonic nanopores will be exploited to achieve single amino-acid optical excitation and enhanced Raman stimulation; ii) ultrafast and ultrasensitive Raman spectrometers will be obtained by combining the emerging technologies of SPAD (Single Photon Avalanche Diode) arrays with dedicated otpica elements which improve the sensitivity and the speed of the detector, and reduce the number of elements of the array necessary to sample a Raman spectrum; iii) bioinformatics approaches will complete the technological platform by developing specific software for discriminating the Raman spectra of proteins with reduced spectral points. Also, insightful experiments on electrophoretic translocation and augmented fluid viscosity in ultra-confined systems will contribute the molecular motion into the nanopores. Finally, to state of the art in-silico design will support the project by contributing to system optimization and data analyses/interpretation.

Field of science

  • /natural sciences/computer and information sciences/data science/data analysis
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins/proteomics
  • /natural sciences/physical sciences/theoretical physics/particle physics/photons

Call for proposal

H2020-FETOPEN-2018-2019-2020-01
See other projects for this call

Funding Scheme

RIA - Research and Innovation action

Coordinator

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Address
Via Morego 30
16163 Genova
Italy
Activity type
Research Organisations
EU contribution
€ 916 031,25

Participants (7)

UNIVERSITE DU LUXEMBOURG
Luxembourg
EU contribution
€ 598 697,50
Address
2 Avenue De L'universite
4365 Esch-sur-alzette
Activity type
Higher or Secondary Education Establishments
KARLSRUHER INSTITUT FUER TECHNOLOGIE
Germany
EU contribution
€ 306 250
Address
Kaiserstrasse 12
76131 Karlsruhe
Activity type
Higher or Secondary Education Establishments
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS
France
EU contribution
€ 225 000
Address
Rue Michel Ange 3
75794 Paris
Activity type
Research Organisations
EUROPEAN MOLECULAR BIOLOGY LABORATORY
Germany
EU contribution
€ 290 950
Address
Meyerhofstrasse 1
69117 Heidelberg
Activity type
Research Organisations
POLITECNICO DI MILANO
Italy
EU contribution
€ 211 460
Address
Piazza Leonardo Da Vinci 32
20133 Milano
Activity type
Higher or Secondary Education Establishments
MICRO PHOTON DEVICES SRL
Italy
EU contribution
€ 190 000
Address
Via Waltraud Gebert Deeg 3/F
39100 Bolzano
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
UNIVERSITA DEGLI STUDI DI PADOVA
Italy
EU contribution
€ 250 125
Address
Via 8 Febbraio 2
35122 Padova
Activity type
Higher or Secondary Education Establishments