A KEY TO THE RATIONAL DESIGN OF EXTRACELLULAR VESICLES-MIMICKING NANOPARTICLES

Información del proyecto

MIMIC-KeY

Identificador del acuerdo de subvención: 964386

DOI

10.3030/964386

Proyecto cerrado

Fecha de la firma de la CE 19 Enero 2021

Fecha de inicio 1 Junio 2021

Fecha de finalización 30 Noviembre 2025

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 2 902 527,50

Aportación de la UE

€ 2 902 527,50

Coordinado por

POLITECNICO DI TORINO
Italy

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (13)

Protocol on synthesis of breakable hard-shell organosilica NPs

The deliverable will consist in a report on the synthesis of hybrid hard organosilica NPs that are cleaved in the presence of specific biomolecules and are able to allow the cargo release

Report on EVs derived from tumor/osteoblastic cells ready and characterized

The deliverable will consist of a report describing the tumorosteoblastic cells chosen as source of EVs and of the protocols used for EV extraction and characterization and related results

Potential of the in vitro cell targeting of artificial EV-mimics

The deliverable will consist of a report on validation of the EV mimics’ performances in binding to bone cells and CTSK delivery

Protocol on the soft artificial nanogel EV-mimics

A report describing the synthesis, characterization and performances of EV mimics based on novel soft nanogel particles

Quantitative estimation of protein numbers and distribution for EVs

The deliverable will consist of a report containing the following parameters: i) size of EVs; ii) number of multiple surface protein per EV; iii) the distribution of these quantities in the EV population.

Kick-off meeting report

Report on the kickoff meeting describing the coordination and partners roles organization of the consortium and of the research work

Protocol for correlative high-resolution analyses

The deliverable will consist of a report describing the developed methodology for the correlation of high-resolution flow cytometric and microscopic analysis, allowing the isolation of different EV populations

Selection of 2 natural EVs bone-targeting candidates

A report describing the use of natural EVs containing CTSK RANKL or other bone targeting candidates for in vivo application on healthy mice to evaluate the biodistribution

Toxicity/safety and biodistribution of secondly-produced EV mimics in k.o. mice models

A report describing the toxicity or safety profiles of the produced EV mimics administered to k.o. mice models up to 8 consecutive doses

Protocol on NP decoration with lipid bilayers and proteins

The deliverable will consist in a report on the formation and self-assembly artificial lipid bilayers and the identified key proteins which should be formulated and used to prepare the shell of EV mimics

Report on in vivo performance of EV-mimic in k.o. mice model.

A report showing the biodistribution and efficiency of the CTSK-containing EV mimics in Ctsk-deficient mice models

Toxicity/safety of the secondly produced EV mimics

A report describing the toxicity or safety profiles of the produced EV mimics administered to healthy animals up to 8 consecutive doses

Quantitative estimation of lipid bilayer characteristics and dynamics in EVs

The deliverable will consist of a report containing quantitative information about lipid bilayer hydrophobicity, polarity, (and therefore composition) and viscosity obtained by single molecule tracking analysis in superresolution

Websites, patent fillings, videos etc. (1)

Website and logo

Website creation and continuous update planningsSocial profiles creation and pages actions and planning for the dissemination to broad publics logo creation and planning for its use

Demonstrators, pilots, prototypes (5)

Molecular models and simulations for lipid-covered hard-core NPs.

The deliverable will consist in a report on modelling and simulations to compare the dynamics of lipid bilayers having the inner leaflet bound to the hard-core NP surface

Molecular models and simulations for lipid-covered hard-core NPs with different geometries

The deliverable will consist in in a report on modelling and simulations to compare the dynamics of lipid bilayers in perfect/flat domains vs. in the presence of discontinuities/edges at the NP surface (NP shape effect)

Molecular models and simulations for EV lipid bilayer envelopes

The deliverable will consist in a report on the modelling and simulations to characterize the dynamics and stability of various EV lipid bilayers envelops.

Predictive models from molecular simulations data

The deliverable will consist in a report describing observed trends and the development of predictive models from advanced analysis of simulation data, useful for the rational experimental customization/synthesis of EVs-mimics

IPR results

List of patented technologies, methods and findings related to the project outcomes

Publicaciones

Peer reviewed articles (22)

Automatic multi-objective optimization of coarse-grained lipid force fields using SwarmCG

Autores: Charly Empereur-mot, Riccardo Capelli, Mattia Perrone, Cristina Caruso, Giovanni Doni, Giovanni M. Pavan
Publicado en: J. Chem. Phys, Edición 156, 2022, ISSN 0021-9606
Editor: American Institute of Physics
DOI: 10.1063/5.0079044

Cargo-loaded lipid-shielded breakable organosilica nanocages for enhanced drug delivery

Autores: María Sancho-Albero, Giada Rosso, Luisa De Cola, Valentina Cauda
Publicado en: Nanoscale, Edición 15, 2023, Página(s) 14628-14640, ISSN 2040-3372
Editor: Royal Society of Chemistry
DOI: 10.1039/d3nr02155f

Acoustically Driven Hybrid Nanocrystals for In Vivo Pancreatic Cancer Treatment

Autores: Marzia Conte, Marco Carofiglio, Robin Shae Vander Pol, Anthony Wood, Nathanael Hernandez, Ashley Joubert, Camden Caffey, Corrine Ying Xuan Chua, Alessandro Grattoni, Valentina Cauda
Publicado en: ACS Applied Materials & Interfaces, Edición 17, 2025, Página(s) 11873-11887, ISSN 1944-8244
Editor: American Chemical Society
DOI: 10.1021/acsami.4c21975

Extracellular Vesicles as Tools for Crossing the Blood–Brain Barrier to Treat Lysosomal Storage Diseases

Autores: Giovanni Lerussi, Verónica Villagrasa-Araya, Marc Moltó-Abad, Mireia del Toro, Guillem Pintos-Morell, Joaquin Seras-Franzoso, Ibane Abasolo
Publicado en: Life, Edición 15, 2025, Página(s) 70, ISSN 2075-1729
Editor: MDPI AG
DOI: 10.3390/life15010070

Targeted lipid-coated ZnO nanoparticles coupled with ultrasound: A sonodynamic approach for the treatment of osteosarcoma as 3D spheroid models

Autores: Marco Carofiglio, Giulia Mesiano, Giada Rosso, Marzia Conte, Marco Zuccheri, Ymera Pignochino, Valentina Cauda
Publicado en: Materials Today Communications, Edición 40, 2024, Página(s) 109826, ISSN 2352-4928
Editor: Elsevier BV
DOI: 10.1016/j.mtcomm.2024.109826

Natural and Artificial Phospholipid Bilayer Coatings on Solid-State Nanoparticles, Current and Future Perspectives

Autores: Bianca Dumontel, Giada Rosso, Valentina Cauda
Publicado en: Nanomedicine, Edición 19, 2024, Página(s) 653-655, ISSN 1743-5889
Editor: Future Medicine Ltd.
DOI: 10.2217/nnm-2023-0358

Spotlight on innovation: key insights from the extracellular vesicle cluster meeting at the European Innovation Council

Autores: Giorgia Adamo, Ioannis Amarantos, Iordanis Arzimanoglou, Paolo Bergese, Antonella Bongiovanni, Benedetta Bussolati, Valentina Cauda, Marcella Chiari, George A. Garinis, Bernd Giebel, Paolo Guazzi, Marcin Jurga, Christian Neri, Pelle Ohlsson, Eva Rohde, Goreti Sales, Irene Trapani, Pieter Vader, Seppo Vainio, Marca H.M. Wauben, María Yáñez-Mó, Natasa Zarovni
Publicado en: Cytotherapy, Edición 28, 2026, Página(s) 101976, ISSN 1465-3249
Editor: Taylor & Francis
DOI: 10.1016/j.jcyt.2025.08.008

Glutathione Threshold‐Triggered Selective Breakability of Organosilica Nanocapsules

Autores: Andrea Mosseri, Ana Rueda‐Flores, Jose L. Hueso, Esteban Urriolabeitia, Luisa De Cola, Jesus Santamaria
Publicado en: ChemCatChem, Edición 18, 2026, ISSN 1867-3880
Editor: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.70141

Biomimicking Extracellular Vesicles with Fully Artificial Ones: A Rational Design of EV-BIOMIMETICS toward Effective Theranostic Tools in Nanomedicine

Autores: Giada Rosso, Valentina Cauda
Publicado en: ACS Biomater. Sci. Eng., Edición 2023, 9, 11, 2023, Página(s) 5924–5932, ISSN 2373-9878
Editor: American Chemical Society
DOI: 10.1021/acsbiomaterials.2c01025

Enhancing Pt(IV) Complexes' Anticancer Activity upon Encapsulation in Stimuli‐Responsive Nanocages

Autores: María Sancho‐Albero, Giorgio Facchetti, Nicolò Panini, Marina Meroni, Ezia Bello, Isabella Rimoldi, Massimo Zucchetti, Roberta Frapolli, Luisa De Cola
Publicado en: Advanced Healthcare Materials, Edición 12, 2025, ISSN 2192-2640
Editor: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202202932

Two Different Responsive Organosilica Nanocarriers to Combine Chemo- and Immunotherapy against Cancer

Autores: Maria Sancho-Albero, Alessia Lucrezia Fenaroli, Mirco Scaccaglia, Cristina Matteo, Chiara Grasselli, Massimo Zucchetti, Roberta Frapolli, Claudia Nastasi, Luisa De Cola
Publicado en: ACS Omega, Edición 9, 2024, Página(s) 41225-41235, ISSN 2470-1343
Editor: American Chemical Society (ACS)
DOI: 10.1021/acsomega.4c02838

Melanoma Extracellular Vesicles Membrane Coated Nanoparticles as Targeted Delivery Carriers for Tumor and Lungs

Autores: María Sancho-Albero, Alessandra Decio, Reha Akpinar, Ada De Luigi, Raffaella Giavazzi, Luigi Maria Terracciano, Luisa De Cola
Publicado en: Materials Today BIO, 2024, ISSN 2590-0064
Editor: Elsevier
DOI: 10.2139/ssrn.5004391

Anti-CD38 targeted nanotrojan horses stimulated by acoustic waves as therapeutic nanotools selectively against Burkitt’s lymphoma cells

Autores: Veronica Vighetto, Marzia Conte, Giada Rosso, Marco Carofiglio, Federica Sidoti Abate, Luisa Racca, Giulia Mesiano, Valentina Cauda
Publicado en: Discover Nano, Edición 19, 2024, ISSN 2731-9229
Editor: Springer Science and Business Media LLC
DOI: 10.1186/s11671-024-03976-z

Exosome-Enhanced Sonodynamic Therapy in Cancer: Emerging Synergies and Modulation of the Tumor Microenvironment

Autores: Giulia Chiabotto, Marzia Conte, Valentina Cauda
Publicado en: Cancers, Edición 18, 2025, Página(s) 118, ISSN 2072-6694
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers18010118

Artificial and Naturally Derived Phospholipidic Bilayers as Smart Coatings of Solid-State Nanoparticles: Current Works and Perspectives in Cancer Therapy

Autores: Nicolò Maria Percivalle; Marco Carofiglio; Marzia Conte; Giada Rosso; Alessandro Bentivogli; Giulia Mesiano; Veronica Vighetto; Valentina Cauda
Publicado en: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Edición 23 (15815), 2022, Página(s) 1-28, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232415815

Lipidic Formulations Inspired by COVID Vaccines as Smart Coatings to Enhance Nanoparticle-Based Cancer Therapy

Autores: Marzia Conte; Marco Carofiglio; Giada Rosso; Valentina Cauda
Publicado en: Nanomaterials; Volume 13; Edición 15; Pages: 2250, Edición 4, 2023, ISSN 2079-4991
Editor: MDPI
DOI: 10.3390/nano13152250

Rational Design of EV-Mimicking Nanoparticles with Polarity-Based Recognition Potential for Advanced Nanocarrier Development

Autores: Giada Rosso, Stijn M.A. Van Veen, María Sancho-Albero, Giulia Tamboia, Charly Empereur-Mot, Claudio Perego, Marije E. Kuipers, Bianca Dumontel, Alessandro Ajó, Esther N. Nolte-’t Hoen, Giovanni M. Pavan, Luisa De Cola, Lorenzo Albertazzi, Valentina Cauda
Publicado en: ACS Applied Nano Materials, Edición 8, 2025, Página(s) 13257-13273, ISSN 2574-0970
Editor: American Chemical Society (ACS)
DOI: 10.1021/acsanm.5c01459

Classifying soft self-assembled materials via unsupervised machine learning of defects

Autores: Andrea Gardin, Claudio Perego, Giovanni Doni, Giovanni M. Pavan
Publicado en: Communications Chemistry, Edición 5, 2022, ISSN 2399-3669
Editor: Springer Science and Business Media LLC
DOI: 10.1038/s42004-022-00699-z

Automatic Optimization of Lipid Models in the Martini Force Field Using <i>SwarmCG</i>

Autores: Charly Empereur-mot, Kasper B. Pedersen, Riccardo Capelli, Martina Crippa, Cristina Caruso, Mattia Perrone, Paulo C. T. Souza, Siewert J. Marrink, Giovanni M. Pavan
Publicado en: Journal of Chemical Information and Modeling, Edición 63, 2023, Página(s) 3827-3838, ISSN 1549-9596
Editor: American Chemical Society
DOI: 10.1021/acs.jcim.3c00530

Acoustic waves and smart biomimetic nanoparticles: combination treatment from 2D to 3D colorectal cancer models

Autores: Giada Rosso, Giulia Mesiano, Bianca Dumontel, Marco Carofiglio, Marzia Conte, Alessandro Grattoni, Valentina Cauda
Publicado en: Cancer Nanotechnology, Edición 15, 2024, ISSN 1868-6958
Editor: Springer Science + Business Media
DOI: 10.1186/s12645-024-00281-3

Designing drug delivery systems: the impact of structural order and disorder for optimized therapeutic outcomes

Autores: Alessandro Masoero, Marzia Conte, Nicolò Maria Percivalle, Elia Pascucci, Giorgia Savino, Veronica Vighetto, Valentina Cauda, Marco Carofiglio
Publicado en: Nanomedicine, Edición 21, 2026, Página(s) 541-572, ISSN 1743-5889
Editor: Future Medicine Ltd.
DOI: 10.1080/17435889.2026.2615102

The Martini 3 Lipidome: Expanded and Refined Parameters Improve Lipid Phase Behavior

Autores: Kasper B. Pedersen, Helgi I. Ingólfsson, Daniel P. Ramirez-Echemendia, Luís Borges-Araújo, Mikkel D. Andreasen, Charly Empereur-mot, Josef Melcr, Tugba N. Ozturk, W. F. Drew Bennett, Lisbeth R. Kjølbye, Christopher Brasnett, Valentina Corradi, Hanif M. Khan, Elio A. Cino, Jackson Crowley, Hyuntae Kim, Balázs Fábián, Ana C. Borges-Araújo, Giovanni M. Pavan, Guillaume Launay, Fabio Lolicato,
Publicado en: ACS Central Science, Edición 11, 2025, Página(s) 1598-1610, ISSN 2374-7943
Editor: American Chemical Society (ACS)
DOI: 10.1021/acscentsci.5c00755

Other (2)

On the Automatic Optimization of Lipid Models in the Martini Force Field using SwarmCG

Autores: Charly Empereur-mot, Kasper B. Pedersen, Cristina Caruso, Mattia Perrone, Martina Crippa, Paulo C.T. Souza, Siewert J. Marrink, Giovanni M. Pavan
Publicado en: PRE-PRINT, 2026
Editor: PRE-PRINT
DOI: 10.26434/chemrxiv-2023-q5rvm

On the Automatic Optimization of Lipid Models in the Martini Force Field using SwarmCG

Autores: Charly Empereur-mot, Kasper B. Pedersen, Riccardo Capelli, Martina Crippa, Cristina Caruso, Mattia Perrone, Paulo C.T. Souza, Siewert J. Marrink, Giovanni M. Pavan
Publicado en: PREPRINT, 2026
Editor: PRE-PRINT
DOI: 10.26434/chemrxiv-2023-q5rvm-v2

Derechos de propiedad intelectual

Patent (2)

COATED NANOPARTICLES AND THEIR USE FOR DELIVERY OF THERAPEUTIC AND DIAGNOSTIC AGENTS

Número de solicitud/publicación: 20 24070607
Fecha: 2024-07-19
Solicitante(s): ISTITUTO DI RICERCHE FARMACOLOGICHE MARIO NEGRI

COATED NANOPARTICLES AND THEIR USE FOR DELIVERY OF THERAPEUTIC AND DIAGNOSTIC AGENTS

Número de solicitud/publicación: 20 24070607
Fecha: 2024-07-19
Solicitante(s): POLITECNICO DI TORINO