A KEY TO THE RATIONAL DESIGN OF EXTRACELLULAR VESICLES-MIMICKING NANOPARTICLES

Leistungen

Protocol on synthesis of breakable hard-shell organosilica NPs

The deliverable will consist in a report on the synthesis of hybrid hard organosilica NPs that are cleaved in the presence of specific biomolecules and are able to allow the cargo release

Report on EVs derived from tumor/osteoblastic cells ready and characterized

The deliverable will consist of a report describing the tumorosteoblastic cells chosen as source of EVs and of the protocols used for EV extraction and characterization and related results

Potential of the in vitro cell targeting of artificial EV-mimics

The deliverable will consist of a report on validation of the EV mimics’ performances in binding to bone cells and CTSK delivery

Protocol on the soft artificial nanogel EV-mimics

A report describing the synthesis, characterization and performances of EV mimics based on novel soft nanogel particles

Quantitative estimation of protein numbers and distribution for EVs

The deliverable will consist of a report containing the following parameters: i) size of EVs; ii) number of multiple surface protein per EV; iii) the distribution of these quantities in the EV population.

Kick-off meeting report

Report on the kickoff meeting describing the coordination and partners roles organization of the consortium and of the research work

Protocol for correlative high-resolution analyses

The deliverable will consist of a report describing the developed methodology for the correlation of high-resolution flow cytometric and microscopic analysis, allowing the isolation of different EV populations

Selection of 2 natural EVs bone-targeting candidates

A report describing the use of natural EVs containing CTSK RANKL or other bone targeting candidates for in vivo application on healthy mice to evaluate the biodistribution

Toxicity/safety and biodistribution of secondly-produced EV mimics in k.o. mice models

A report describing the toxicity or safety profiles of the produced EV mimics administered to k.o. mice models up to 8 consecutive doses

Protocol on NP decoration with lipid bilayers and proteins

The deliverable will consist in a report on the formation and self-assembly artificial lipid bilayers and the identified key proteins which should be formulated and used to prepare the shell of EV mimics

Report on in vivo performance of EV-mimic in k.o. mice model.

A report showing the biodistribution and efficiency of the CTSK-containing EV mimics in Ctsk-deficient mice models

Toxicity/safety of the secondly produced EV mimics

A report describing the toxicity or safety profiles of the produced EV mimics administered to healthy animals up to 8 consecutive doses

Quantitative estimation of lipid bilayer characteristics and dynamics in EVs

The deliverable will consist of a report containing quantitative information about lipid bilayer hydrophobicity, polarity, (and therefore composition) and viscosity obtained by single molecule tracking analysis in superresolution

Website and logo

Website creation and continuous update planningsSocial profiles creation and pages actions and planning for the dissemination to broad publics logo creation and planning for its use

Molecular models and simulations for lipid-covered hard-core NPs.

The deliverable will consist in a report on modelling and simulations to compare the dynamics of lipid bilayers having the inner leaflet bound to the hard-core NP surface

Molecular models and simulations for lipid-covered hard-core NPs with different geometries

The deliverable will consist in in a report on modelling and simulations to compare the dynamics of lipid bilayers in perfect/flat domains vs. in the presence of discontinuities/edges at the NP surface (NP shape effect)

Molecular models and simulations for EV lipid bilayer envelopes

The deliverable will consist in a report on the modelling and simulations to characterize the dynamics and stability of various EV lipid bilayers envelops.

Predictive models from molecular simulations data

The deliverable will consist in a report describing observed trends and the development of predictive models from advanced analysis of simulation data, useful for the rational experimental customization/synthesis of EVs-mimics

IPR results

List of patented technologies, methods and findings related to the project outcomes

Veröffentlichungen

Automatic multi-objective optimization of coarse-grained lipid force fields using SwarmCG

Autoren: Charly Empereur-mot, Riccardo Capelli, Mattia Perrone, Cristina Caruso, Giovanni Doni, Giovanni M. Pavan
Veröffentlicht in: J. Chem. Phys, Ausgabe 156, 2022, ISSN 0021-9606
Herausgeber: American Institute of Physics
DOI: 10.1063/5.0079044

Cargo-loaded lipid-shielded breakable organosilica nanocages for enhanced drug delivery

Autoren: María Sancho-Albero, Giada Rosso, Luisa De Cola, Valentina Cauda
Veröffentlicht in: Nanoscale, Ausgabe 15, 2023, Seite(n) 14628-14640, ISSN 2040-3372
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/d3nr02155f

Acoustically Driven Hybrid Nanocrystals for In Vivo Pancreatic Cancer Treatment

Autoren: Marzia Conte, Marco Carofiglio, Robin Shae Vander Pol, Anthony Wood, Nathanael Hernandez, Ashley Joubert, Camden Caffey, Corrine Ying Xuan Chua, Alessandro Grattoni, Valentina Cauda
Veröffentlicht in: ACS Applied Materials & Interfaces, Ausgabe 17, 2025, Seite(n) 11873-11887, ISSN 1944-8244
Herausgeber: American Chemical Society
DOI: 10.1021/acsami.4c21975

Extracellular Vesicles as Tools for Crossing the Blood–Brain Barrier to Treat Lysosomal Storage Diseases

Autoren: Giovanni Lerussi, Verónica Villagrasa-Araya, Marc Moltó-Abad, Mireia del Toro, Guillem Pintos-Morell, Joaquin Seras-Franzoso, Ibane Abasolo
Veröffentlicht in: Life, Ausgabe 15, 2025, Seite(n) 70, ISSN 2075-1729
Herausgeber: MDPI AG
DOI: 10.3390/life15010070

Targeted lipid-coated ZnO nanoparticles coupled with ultrasound: A sonodynamic approach for the treatment of osteosarcoma as 3D spheroid models

Autoren: Marco Carofiglio, Giulia Mesiano, Giada Rosso, Marzia Conte, Marco Zuccheri, Ymera Pignochino, Valentina Cauda
Veröffentlicht in: Materials Today Communications, Ausgabe 40, 2024, Seite(n) 109826, ISSN 2352-4928
Herausgeber: Elsevier BV
DOI: 10.1016/j.mtcomm.2024.109826

Natural and Artificial Phospholipid Bilayer Coatings on Solid-State Nanoparticles, Current and Future Perspectives

Autoren: Bianca Dumontel, Giada Rosso, Valentina Cauda
Veröffentlicht in: Nanomedicine, Ausgabe 19, 2024, Seite(n) 653-655, ISSN 1743-5889
Herausgeber: Future Medicine Ltd.
DOI: 10.2217/nnm-2023-0358

Spotlight on innovation: key insights from the extracellular vesicle cluster meeting at the European Innovation Council

Autoren: Giorgia Adamo, Ioannis Amarantos, Iordanis Arzimanoglou, Paolo Bergese, Antonella Bongiovanni, Benedetta Bussolati, Valentina Cauda, Marcella Chiari, George A. Garinis, Bernd Giebel, Paolo Guazzi, Marcin Jurga, Christian Neri, Pelle Ohlsson, Eva Rohde, Goreti Sales, Irene Trapani, Pieter Vader, Seppo Vainio, Marca H.M. Wauben, María Yáñez-Mó, Natasa Zarovni
Veröffentlicht in: Cytotherapy, Ausgabe 28, 2026, Seite(n) 101976, ISSN 1465-3249
Herausgeber: Taylor & Francis
DOI: 10.1016/j.jcyt.2025.08.008

Glutathione Threshold‐Triggered Selective Breakability of Organosilica Nanocapsules

Autoren: Andrea Mosseri, Ana Rueda‐Flores, Jose L. Hueso, Esteban Urriolabeitia, Luisa De Cola, Jesus Santamaria
Veröffentlicht in: ChemCatChem, Ausgabe 18, 2026, ISSN 1867-3880
Herausgeber: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.70141

Biomimicking Extracellular Vesicles with Fully Artificial Ones: A Rational Design of EV-BIOMIMETICS toward Effective Theranostic Tools in Nanomedicine

Autoren: Giada Rosso, Valentina Cauda
Veröffentlicht in: ACS Biomater. Sci. Eng., Ausgabe 2023, 9, 11, 2023, Seite(n) 5924–5932, ISSN 2373-9878
Herausgeber: American Chemical Society
DOI: 10.1021/acsbiomaterials.2c01025

Enhancing Pt(IV) Complexes' Anticancer Activity upon Encapsulation in Stimuli‐Responsive Nanocages

Autoren: María Sancho‐Albero, Giorgio Facchetti, Nicolò Panini, Marina Meroni, Ezia Bello, Isabella Rimoldi, Massimo Zucchetti, Roberta Frapolli, Luisa De Cola
Veröffentlicht in: Advanced Healthcare Materials, Ausgabe 12, 2025, ISSN 2192-2640
Herausgeber: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202202932

Two Different Responsive Organosilica Nanocarriers to Combine Chemo- and Immunotherapy against Cancer

Autoren: Maria Sancho-Albero, Alessia Lucrezia Fenaroli, Mirco Scaccaglia, Cristina Matteo, Chiara Grasselli, Massimo Zucchetti, Roberta Frapolli, Claudia Nastasi, Luisa De Cola
Veröffentlicht in: ACS Omega, Ausgabe 9, 2024, Seite(n) 41225-41235, ISSN 2470-1343
Herausgeber: American Chemical Society (ACS)
DOI: 10.1021/acsomega.4c02838

Melanoma Extracellular Vesicles Membrane Coated Nanoparticles as Targeted Delivery Carriers for Tumor and Lungs

Autoren: María Sancho-Albero, Alessandra Decio, Reha Akpinar, Ada De Luigi, Raffaella Giavazzi, Luigi Maria Terracciano, Luisa De Cola
Veröffentlicht in: Materials Today BIO, 2024, ISSN 2590-0064
Herausgeber: Elsevier
DOI: 10.2139/ssrn.5004391

Anti-CD38 targeted nanotrojan horses stimulated by acoustic waves as therapeutic nanotools selectively against Burkitt’s lymphoma cells

Autoren: Veronica Vighetto, Marzia Conte, Giada Rosso, Marco Carofiglio, Federica Sidoti Abate, Luisa Racca, Giulia Mesiano, Valentina Cauda
Veröffentlicht in: Discover Nano, Ausgabe 19, 2024, ISSN 2731-9229
Herausgeber: Springer Science and Business Media LLC
DOI: 10.1186/s11671-024-03976-z

Exosome-Enhanced Sonodynamic Therapy in Cancer: Emerging Synergies and Modulation of the Tumor Microenvironment

Autoren: Giulia Chiabotto, Marzia Conte, Valentina Cauda
Veröffentlicht in: Cancers, Ausgabe 18, 2025, Seite(n) 118, ISSN 2072-6694
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers18010118

Artificial and Naturally Derived Phospholipidic Bilayers as Smart Coatings of Solid-State Nanoparticles: Current Works and Perspectives in Cancer Therapy

Autoren: Nicolò Maria Percivalle; Marco Carofiglio; Marzia Conte; Giada Rosso; Alessandro Bentivogli; Giulia Mesiano; Veronica Vighetto; Valentina Cauda
Veröffentlicht in: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Ausgabe 23 (15815), 2022, Seite(n) 1-28, ISSN 1422-0067
Herausgeber: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms232415815

Lipidic Formulations Inspired by COVID Vaccines as Smart Coatings to Enhance Nanoparticle-Based Cancer Therapy

Autoren: Marzia Conte; Marco Carofiglio; Giada Rosso; Valentina Cauda
Veröffentlicht in: Nanomaterials; Volume 13; Ausgabe 15; Pages: 2250, Ausgabe 4, 2023, ISSN 2079-4991
Herausgeber: MDPI
DOI: 10.3390/nano13152250

Rational Design of EV-Mimicking Nanoparticles with Polarity-Based Recognition Potential for Advanced Nanocarrier Development

Autoren: Giada Rosso, Stijn M.A. Van Veen, María Sancho-Albero, Giulia Tamboia, Charly Empereur-Mot, Claudio Perego, Marije E. Kuipers, Bianca Dumontel, Alessandro Ajó, Esther N. Nolte-’t Hoen, Giovanni M. Pavan, Luisa De Cola, Lorenzo Albertazzi, Valentina Cauda
Veröffentlicht in: ACS Applied Nano Materials, Ausgabe 8, 2025, Seite(n) 13257-13273, ISSN 2574-0970
Herausgeber: American Chemical Society (ACS)
DOI: 10.1021/acsanm.5c01459

Classifying soft self-assembled materials via unsupervised machine learning of defects

Autoren: Andrea Gardin, Claudio Perego, Giovanni Doni, Giovanni M. Pavan
Veröffentlicht in: Communications Chemistry, Ausgabe 5, 2022, ISSN 2399-3669
Herausgeber: Springer Science and Business Media LLC
DOI: 10.1038/s42004-022-00699-z

Automatic Optimization of Lipid Models in the Martini Force Field Using <i>SwarmCG</i>

Autoren: Charly Empereur-mot, Kasper B. Pedersen, Riccardo Capelli, Martina Crippa, Cristina Caruso, Mattia Perrone, Paulo C. T. Souza, Siewert J. Marrink, Giovanni M. Pavan
Veröffentlicht in: Journal of Chemical Information and Modeling, Ausgabe 63, 2023, Seite(n) 3827-3838, ISSN 1549-9596
Herausgeber: American Chemical Society
DOI: 10.1021/acs.jcim.3c00530

Acoustic waves and smart biomimetic nanoparticles: combination treatment from 2D to 3D colorectal cancer models

Autoren: Giada Rosso, Giulia Mesiano, Bianca Dumontel, Marco Carofiglio, Marzia Conte, Alessandro Grattoni, Valentina Cauda
Veröffentlicht in: Cancer Nanotechnology, Ausgabe 15, 2024, ISSN 1868-6958
Herausgeber: Springer Science + Business Media
DOI: 10.1186/s12645-024-00281-3

Designing drug delivery systems: the impact of structural order and disorder for optimized therapeutic outcomes

Autoren: Alessandro Masoero, Marzia Conte, Nicolò Maria Percivalle, Elia Pascucci, Giorgia Savino, Veronica Vighetto, Valentina Cauda, Marco Carofiglio
Veröffentlicht in: Nanomedicine, Ausgabe 21, 2026, Seite(n) 541-572, ISSN 1743-5889
Herausgeber: Future Medicine Ltd.
DOI: 10.1080/17435889.2026.2615102

The Martini 3 Lipidome: Expanded and Refined Parameters Improve Lipid Phase Behavior

Autoren: Kasper B. Pedersen, Helgi I. Ingólfsson, Daniel P. Ramirez-Echemendia, Luís Borges-Araújo, Mikkel D. Andreasen, Charly Empereur-mot, Josef Melcr, Tugba N. Ozturk, W. F. Drew Bennett, Lisbeth R. Kjølbye, Christopher Brasnett, Valentina Corradi, Hanif M. Khan, Elio A. Cino, Jackson Crowley, Hyuntae Kim, Balázs Fábián, Ana C. Borges-Araújo, Giovanni M. Pavan, Guillaume Launay, Fabio Lolicato,
Veröffentlicht in: ACS Central Science, Ausgabe 11, 2025, Seite(n) 1598-1610, ISSN 2374-7943
Herausgeber: American Chemical Society (ACS)
DOI: 10.1021/acscentsci.5c00755

On the Automatic Optimization of Lipid Models in the Martini Force Field using SwarmCG

Autoren: Charly Empereur-mot, Kasper B. Pedersen, Cristina Caruso, Mattia Perrone, Martina Crippa, Paulo C.T. Souza, Siewert J. Marrink, Giovanni M. Pavan
Veröffentlicht in: PRE-PRINT, 2026
Herausgeber: PRE-PRINT
DOI: 10.26434/chemrxiv-2023-q5rvm

On the Automatic Optimization of Lipid Models in the Martini Force Field using SwarmCG

Autoren: Charly Empereur-mot, Kasper B. Pedersen, Riccardo Capelli, Martina Crippa, Cristina Caruso, Mattia Perrone, Paulo C.T. Souza, Siewert J. Marrink, Giovanni M. Pavan
Veröffentlicht in: PREPRINT, 2026
Herausgeber: PRE-PRINT
DOI: 10.26434/chemrxiv-2023-q5rvm-v2

Rechte des geistigen Eigentums

COATED NANOPARTICLES AND THEIR USE FOR DELIVERY OF THERAPEUTIC AND DIAGNOSTIC AGENTS

Antrags-/Publikationsnummer: 20 24070607
Datum: 2024-07-19
Antragsteller: ISTITUTO DI RICERCHE FARMACOLOGICHE MARIO NEGRI

COATED NANOPARTICLES AND THEIR USE FOR DELIVERY OF THERAPEUTIC AND DIAGNOSTIC AGENTS

Antrags-/Publikationsnummer: 20 24070607
Datum: 2024-07-19
Antragsteller: POLITECNICO DI TORINO

Leistungen

Veröffentlichungen

Rechte des geistigen Eigentums

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