ENable LIGHT- and synthetic biology-driven volumetric bioprinting of functional human tissues

Informazioni relative al progetto

ENLIGHT

ID dell’accordo di sovvenzione: 964497

DOI

10.3030/964497

Progetto chiuso

Data della firma CE 22 Gennaio 2021

Data di avvio 1 Maggio 2021

Data di completamento 30 Aprile 2025

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 3 594 925,00

Contributo UE

€ 3 594 925,00

Coordinato da

UNIVERSITAIR MEDISCH CENTRUM UTRECHT
Netherlands

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (3)

RRI analysis report

The outcomes of the analysis and workshop at the end of the project will be reported.Task involved: T6.3

Infographs describing the project and its impact

After the first year infographs of the project and its impact will be made public through the website The infographs will be updated on the website throughout the projectTask involved T62

Management handbook

A management handbook will be written including templates meeting schedules and a detailed planning of activities as well as decisionmaking processesTasks involved T71 and T72

Websites, patent fillings, videos etc. (1)

ENLIGHT logo and website

The ENLIGHT logo and first version of the project website will be delivered at M2Task involved T62

Pubblicazioni

Peer reviewed articles (37)

Smartphone-Flashlight-Mediated Remote Control of Rapid Insulin Secretion Restores Glucose Homeostasis in Experimental Type-1 Diabetes

Autori: Mansouri, Maysam; Xue, Shuai; Hussherr, Marie-Didiée; Strittmatter, Tobias; Camenisch, Gieri; Fussenegger, Martin
Pubblicato in: Small, Numero 4, 2021, ISSN 1613-6829
Editore: Wiley
DOI: 10.1002/smll.202101939

3D<i>in vitro</i>modeling of the exocrine pancreatic unit using tomographic volumetric bioprinting

Autori: Viola Sgarminato, Jorge Madrid-Wolff, Antoine Boniface, Gianluca Ciardelli, Chiara Tonda-Turo, Christophe Moser
Pubblicato in: Biofabrication, 2024, ISSN 1758-5090
Editore: IOP Publishing Ltd
DOI: 10.1101/2023.01.23.525142

Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Autori: Chansoria, Parth; id_orcid0000-0002-6107-6848; Rizzo, Riccardo; id_orcid0000-0001-8297-6776; Rütsche, Dominic; Liu, Hao; Delrot, Paul; Zenobi-Wong, Marcy; id_orcid0000-0002-8522-9909
Pubblicato in: Chemical Reviews, 124 (14), Numero 1, 2024, ISSN 0009-2665
Editore: American Chemical Society

Bioprinting of Human Liver‐Derived Epithelial Organoids for Toxicity Studies

Autori: Manon C. Bouwmeester, Paulina N. Bernal, Loes A. Oosterhoff, Monique E. van Wolferen, Vivian Lehmann, Monique Vermaas, Maj‐Britt Buchholz, Quentin C. Peiffer, Jos Malda, Luc J. W. van der Laan, Nynke I. Kramer, Kerstin Schneeberger, Riccardo Levato, Bart Spee
Pubblicato in: Macromolecular Bioscience, Numero 21, 2023, ISSN 1616-5187
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/mabi.202100327

Liver Organoids: Updates on Disease Modeling and Biomedical Applications

Autori: Carmen Caiazza, Silvia Parisi, Massimiliano Caiazzo
Pubblicato in: Biology, Numero 10, 2024, Pagina/e 835, ISSN 2079-7737
Editore: MDPI AG
DOI: 10.3390/biology10090835

Liver Organoids: Updates on Disease Modeling and Biomedical Applications.

Autori: Caiazza, Carmen; Parisi, Silvia; Caiazzo, Massimiliano; Afd Pharmaceutics; Pharmaceutics
Pubblicato in: Biology, Numero 1, 2021, ISSN 2079-7737
Editore: MDPI
DOI: 10.3390/biology10090835

Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactories

Autori: Paulina; Manon; Jorge; Marc; Sammy; Nuria; Gabriel; Roos-Anne; Monique; Luc; Paul; Damien; Jos; Christophe; Bart; Riccardo
Pubblicato in: Advanced Materials, Numero 1, 2022, ISSN 1521-4095
Editore: Wiley-VCH
DOI: 10.5281/zenodo.6380100

Receptor-mediated transcytosis of nanobodies targeting the heparin-binding EGF-like growth factor in human blood-brain barrier models

Autori: Boning Qiu, Sara Pompe, Katerina T. Xenaki, Alessia Di Maggio, Clara Belinchón Moreno, Paul M.P. van Bergen en Henegouwen, Enrico Mastrobattista, Sabrina Oliveira, Massimiliano Caiazzo
Pubblicato in: Journal of Controlled Release, Numero 383, 2025, Pagina/e 113852, ISSN 0168-3659
Editore: Elsevier BV
DOI: 10.1016/j.jconrel.2025.113852

Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteins

Autori: Mohamed Mahameed, Shuai Xue, Benjamin Danuser, Ghislaine Charpin-El Hamri, Mingqi Xie, Martin Fussenegger
Pubblicato in: Nature Biomedical Engineering, 2025, ISSN 2157-846X
Editore: Springer Science and Business Media LLC
DOI: 10.1038/s41551-025-01350-7

Glucose‐Operated Widget (GLOW) for Closed‐Loop Optogenetic Glycemic Control

Autori: Debasis Maity, Preetam Guha Ray, Martin Fussenegger
Pubblicato in: Advanced Materials, Numero 36, 2024, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202408537

Light-based vat-polymerization bioprinting

Autori: Riccardo Levato, Oksana Dudaryeva, Carlos Ezio Garciamendez-Mijares, Bruce E. Kirkpatrick, Riccardo Rizzo, Jacob Schimelman, Kristi S. Anseth, Shaochen Chen, Marcy Zenobi-Wong, Yu Shrike Zhang
Pubblicato in: Nature Reviews Methods Primers, Numero 3, 2024, ISSN 2662-8449
Editore: Nature
DOI: 10.1038/s43586-023-00231-0

Light-based vat-polymerization bioprinting

Autori: Riccardo Levato; Oksana Dudaryeva; Carlos Ezio Garciamendez-Mijares; Bruce E. Kirkpatrick; Riccardo Rizzo; Jacob Schimelman; Kristi S. Anseth; Shaochen Chen; Marcy Zenobi-Wong; Yu Shrike Zhang
Pubblicato in: Nature Reviews Methods Primers, 3, Numero 1, 2023, ISSN 2662-8449
Editore: Nature
DOI: 10.1038/s43586-023-00231-0

Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion-Volumetric Printing of Microgels

Autori: Davide Ribezzi, Marième Gueye, Sammy Florczak, Franziska Dusi, Dieuwke de Vos, Francesca Manente, Andreas Hierholzer, Martin Fussenegger, Massimiliano Caiazzo, Torsten Blunk, Jos Malda, Riccardo Levato
Pubblicato in: Advanced Materials, 2023, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202301673

Engineering electrogenetic interfaces for mammalian cell control

Autori: Maysam Mansouri, Martin Fussenegger
Pubblicato in: Cell Chemical Biology, Numero 32, 2025, Pagina/e 521-528, ISSN 2451-9456
Editore: Elsevier BV
DOI: 10.1016/j.chembiol.2025.01.003

A review of materials used in tomographic volumetric additive manufacturing

Autori: Jorge Madrid-Wolff, Joseph Toombs, Riccardo Rizzo, Paulina Nuñez Bernal, Dominique Porcincula, Rebecca Walton, Bin Wang, Frederik Kotz-Helmer, Yi Yang, David Kaplan, Yu Shrike Zhang, Marcy Zenobi-Wong, Robert R. McLeod, Bastian Rapp, Johanna Schwartz, Maxim Shusteff, Hayden Talyor, Riccardo Levato, Christophe Moser
Pubblicato in: MRS Communications, Numero 13, 2023, Pagina/e 764-785, ISSN 2159-6867
Editore: Springer Science and Business Media LLC
DOI: 10.1557/s43579-023-00447-x

Smart-watch-programmed green-light-operated percutaneous control of therapeutic transgenes

Autori: Maysam Mansouri, Marie-Didiée Hussherr, Tobias Strittmatter, Peter Buchmann, Shuai Xue, Gieri Camenisch, Martin Fussenegger
Pubblicato in: Nature Communications, Numero 12/1, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-021-23572-4

Enhancing cell‐based therapies with synthetic gene circuits responsive to molecular stimuli

Autori: Silvia Galvan, Ana P. Teixeira, Martin Fussenegger
Pubblicato in: Biotechnology and Bioengineering, Numero 121, 2025, Pagina/e 2987-3000, ISSN 0006-3592
Editore: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/bit.28770

Bioprinting and synthetic biology approaches to engineer functional endocrine pancreatic constructs

Autori: Davide Ribezzi, Pere Català, Cataldo Pignatelli, Antonio Citro, Riccardo Levato
Pubblicato in: Trends in Biotechnology, 2025, ISSN 0167-7799
Editore: Elsevier BV
DOI: 10.1016/j.tibtech.2025.03.005

Delivery Systems in Neuronal Direct Cell Reprogramming

Autori: Giulia Redi, Filomena Del Piano, Sara Cappellini, Martina Paladino, Anne den Breejen, Marcel H.A.M. Fens, Massimiliano Caiazzo
Pubblicato in: Cellular Reprogramming, 2025, ISSN 2152-4971
Editore: Mary Ann Liebert Inc.
DOI: 10.1089/cell.2025.0008

Brain Organoids: Filling the Need for a Human Model of Neurological Disorder

Autori: Philip Jalink, Massimiliano Caiazzo
Pubblicato in: Biology, Numero 10, 2025, Pagina/e 740, ISSN 2079-7737
Editore: MDPI
DOI: 10.3390/biology10080740

A review of materials used in tomographic volumetric additive manufacturing

Autori: Jorge Madrid-Wolff; Joseph Toombs; Riccardo Rizzo; Paulina Nuñez Bernal; Dominique Porcincula; Rebecca Walton; Bin Wang; Frederik Kotz-Helmer; Yi Yang; David Kaplan; Yu Shrike Zhang; Marcy Zenobi-Wong; Robert R. McLeod; Bastian Rapp; Johanna Schwartz; Maxim Shusteff; Hayden Talyor; Riccardo Levato; Christophe Moser
Pubblicato in: MRS Communications, Numero 3, 2023, ISSN 2159-6867
Editore: Springer Link
DOI: 10.1557/s43579-023-00447-x

MicroRNA Roles in Cell Reprogramming Mechanisms

Autori: Pascale, Emilia; Caiazza, Carmen; Paladino, Martina; Parisi, Silvia; Passaro, Fabiana; Caiazzo, Massimiliano
Pubblicato in: Cells, Numero 3, 2022, ISSN 2073-4409
Editore: MDPI
DOI: 10.3390/cells11060940

Breaking the resolution limits of 3D bioprinting: future opportunities and present challenges

Autori: Tommaso Zandrini, Sammy Florczak, Riccardo Levato, and Aleksandr Ovsianikov
Pubblicato in: Trends in Biotechnology, 2022, ISSN 0167-7799
Editore: Elsevier BV
DOI: 10.1016/j.tibtech.2022.10.009

Induced pluripotent stem cell-derived and directly reprogrammed neurons to study neurodegenerative diseases: The impact of aging signatures

Autori: Simona Aversano, Carmen Caiazza, Massimiliano Caiazzo
Pubblicato in: Frontiers in Aging Neuroscience, 2022, ISSN 1663-4365
Editore: Frontiers Research Foundation
DOI: 10.3389/fnagi.2022.1069482

Levodopa-loaded nanoparticles for the treatment of Parkinson's disease

Autori: Emile F. van Vliet, Maarten J. Knol, Raymond M. Schiffelers, Massimiliano Caiazzo, Marcel H.A.M. Fens
Pubblicato in: Journal of Controlled Release, Numero 360, 2023, Pagina/e 212-224, ISSN 0168-3659
Editore: Elsevier BV
DOI: 10.1016/j.jconrel.2023.06.026

Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactor

Autori: Paulina Nuñez Bernal,Manon Bouwmeester,Jorge Madrid-Wolff,Marc Falandt,Sammy Florczak,Nuria Ginés Rodriguez,Yang Li,Gabriel Größbacher,Roos-Anne Samsom,Monique van Wolferen,Luc J. W. van der Laan,Paul Delrot,Damien Loterie,Jos Malda,Christophe Moser,Bart Spee,Riccardo Levato
Pubblicato in: Advanced Materials, 2022, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202110054

Programming Delayed Dissolution Into Sacrificial Bioinks For Dynamic Temporal Control of Architecture within 3D-Bioprinted Constructs

Autori: Bram G. Soliman, Alessia Longoni, Mian Wang, Wanlu Li, Paulina N. Bernal, Alessandro Cianciosi, Gabriella C.J. Lindberg, Jos Malda, Juergen Groll, Tomasz Jungst, Riccardo Levato, Jelena Rnjak-Kovacina, Tim B. F. Woodfield, Yu Shrike Zhang, Khoon S. Lim
Pubblicato in: Advanced Functional Materials, 2023, ISSN 1616-301X
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/adfm.202210521

Design of programmable post-translational switch control platform for on-demand protein secretion in mammalian cells

Autori: Maysam Mansouri, Preetam Guha Ray, Nik Franko, Shuai Xue, Martin Fussenegger
Pubblicato in: Nucleic Acids Research, 2023, ISSN 1362-4962
Editore: Oxford University Press
DOI: 10.1093/nar/gkac916

Controlling Light in Scattering Materials for Volumetric Additive Manufacturing

Autori: Madrid-Wolff, Jorge; Boniface, Antoine; Loterie, Damien; Delrot, Paul; Moser, Christophe
Pubblicato in: Advanced Science, Numero 1, 2022, ISSN 2198-3844
Editore: Wiley
DOI: 10.1002/advs.202105144

Therapeutic cell engineering: designing programmable synthetic genetic circuits in mammalian cells

Autori: Maysam Mansouri, Martin Fussenegger
Pubblicato in: Protein &amp; Cell, Numero 13, 2023, Pagina/e 476-489, ISSN 1674-8018
Editore: Oxford University Press (OUP)
DOI: 10.1007/s13238-021-00876-1

Small‐Molecule Regulators for Gene Switches to Program Mammalian Cell Behaviour

Autori: Maysam Mansouri, Martin Fussenegger
Pubblicato in: ChemBioChem, Numero 25, 2025, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202300717

Light from Afield: Fast, High-Resolution, and Layer-Free Deep Vat 3D Printing

Autori: Parth Chansoria, Riccardo Rizzo, Dominic Rütsche, Hao Liu, Paul Delrot, Marcy Zenobi-Wong
Pubblicato in: Chemical Reviews, Numero 124, 2024, Pagina/e 8787-8822, ISSN 0009-2665
Editore: American Chemical Society
DOI: 10.1021/acs.chemrev.4c00134

Engineering receptors in the secretory pathway for orthogonal signalling control

Autori: Mohamed Mahameed, Pengli Wang, Shuai Xue & Martin Fussenegger
Pubblicato in: Nature Communications, 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-35161-0

Volumetric Printing Across Melt Electrowritten Scaffolds Fabricates Multi-Material Living Constructs with Tunable Architecture and Mechanics

Autori: Gabriel Größbacher, Michael Bartolf-Kopp, Csaba Gergely, Paulina Núñez Bernal, Sammy Florczak, Mylène de Ruijter, Núria Ginés Rodriguez, Jürgen Groll, Jos Malda, Tomasz Jungst, Riccardo Levato
Pubblicato in: Advanced Materials, 2023, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202300756

Inverse Rendering for Tomographic Volumetric Additive Manufacturing

Autori: Baptiste Nicolet, Felix Wechsler, Jorge Madrid-Wolff, Christophe Moser, Wenzel Jakob
Pubblicato in: ACM Transactions on Graphics, Numero 43, 2024, Pagina/e 1-17, ISSN 0730-0301
Editore: Association for Computing Machinary, Inc.
DOI: 10.1145/3687924

The road ahead in materials and technologies for volumetric 3D printing

Autori: Paulina Nunez Bernal, Sammy Florczak, Sebastian Inacker, Xiao Kuang, Jorge Madrid-Wolff, Martin Regehly, Stefan Hecht, Yu Shrike Zhang, Christophe Moser, Riccardo Levato
Pubblicato in: Nature Reviews Materials, 2025, ISSN 2058-8437
Editore: Springer Science and Business Media LLC
DOI: 10.1038/s41578-025-00785-3

Biofabricating the vascular tree in engineered bone tissue

Autori: Leanne de Silva, Paulina N. Bernal, AJW Rosenberg, Jos Malda, Riccardo Levato, Debby Gawlitta
Pubblicato in: Acta Biomaterialia, 2023, Pagina/e 250-268, ISSN 1742-7061
Editore: Elsevier BV
DOI: 10.1016/j.actbio.2022.08.051

Other (1)

Exploring RRI in Bioprinted Organoids: Reflections from the ENLIGHT project

Autori: Simone, Angela; Pellizzone, Anna
Pubblicato in: Numero 1, 2025
Editore: Zenodo
DOI: 10.5281/zenodo.15372410

Book chapters (1)

Posttranslational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells

Autori: Maysam Mansouri, Martin Fussenegger
Pubblicato in: Methods in Molecular Biology, Mammalian Synthetic Systems, 2024, Pagina/e 233-241, ISBN 978-1-0716-3718-0
Editore: Springer US
DOI: 10.1007/978-1-0716-3718-0_15

Non-peer reviewed articles (1)

Multi‐material Volumetric Bioprinting and Plug‐and‐play Suspension Bath Biofabrication via Bioresin Molecular Weight Tuning and via Multiwavelength Alignment Optics

Autori: Davide Ribezzi, Jan‐Philip Zegwaart, Thomas Van Gansbeke, Aitor Tejo‐Otero, Sammy Florczak, Joska Aerts, Paul Delrot, Andreas Hierholzer, Martin Fussenegger, Jos Malda, Jos Olijve, Riccardo Levato
Pubblicato in: Advanced Materials, Numero 37, 2025, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202409355