Synthetic Biology of mRNA

Informazioni relative al progetto

NEWmRNA

ID dell’accordo di sovvenzione: 965135

DOI

10.3030/965135

Progetto chiuso

Data della firma CE 16 Dicembre 2020

Data di avvio 1 Marzo 2021

Data di completamento 31 Agosto 2025

Finanziato da

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Costo totale

€ 2 998 545,00

Contributo UE

€ 2 998 545,00

Coordinato da

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

Documents, reports (13)

Synthesis of novel nucleoside triphosphates, part I

Report on nonconfidential synthesis of novel nucleoside triphosphates with modified bases focus on pyrimidinelike bases

Partially operative T7 RNAPol variant

Report on nonconfidential aspects of first partially operative T7 RNAPol variants

Synthesis of novel nucleoside triphosphates for in vitro transcription

Report on nonconfidential aspects of synthesis of novel nucleoside triphosphates with noncanonical sugars for in vitro transcription U and Ccarrying bases

Dissemination and exploitation plan
Chemical assembly of short mRNAs, part I

Report on nonconfidential aspects of chemical assembly of short mRNAs

Report on .eu project website and logo

Report on eu project website and logo M2 ETH Zrich

Enzyme with hydrolytic activity for cNMP

Summary report on nonconfidential aspects of enzyme development for deblocking cNMP

CNA precursor uptake

Report on nonconfidential aspects of import engineering for CNA precursors

Robust E. coli chassis lacking phosphoribose and PRPP metabolism

Summary report on nonconfidential aspects of engineering PRPP metabolismfree E coli

Production of circRNA in E. coli (short RNA)

Protocol for efficient production of short circRNA production in E coli

Synthesis of transport and in vivo transcription substrates, part I

Report on nonconfidential aspects of synthesis of transport and in vivo transcription substrates using carbamate cNTPs focus on U and Cbases

Establishment of T7 RNAPol mutant libraries and HTP screen

Report on the establishment of T7 RNAPol mutant libraries and a suitable HTP screen

Characterization of in vivo functionality of oligonucleotides, part I

Preliminary summary report on nonconfidential aspects of the characterization of in vivo functionality of oligonucleotides with noncanonical pyrimidine or purine base moieties

Open Research Data Pilot (1)

Data management plan

Report on data management plan

Pubblicazioni

Peer reviewed articles (13)

A microbiological system for screening the interference of XNA monomers with DNA and RNA metabolism.

Autori: Blanchard, A., Abramov, M., Hassan, C., Marliere, P., Herdewijn, P., Pezo, V. A
Pubblicato in: RSC Advances, 2023, ISSN 2046-2069
Editore: Royal Society of Chemistry
DOI: 10.1039/d3ra06172h

In vivo assembly and expression of DNA containing non-canonical bases in the yeast Saccharomyces cerevisiae.

Autori: Nguyen, H., Abramov, M., Rozenski, J., Ermeeva, E., Herdewijn, P.
Pubblicato in: ChemBioChem, 2023, ISSN 1439-4227
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202200060

LL37 complexed to double-stranded RNA induces RIG-I-like receptor signalling and Gasdermin E activation facilitating IL-36γ release from keratinocytes

Autori: Jennifer Keller, Judit Danis, Isabella Krehl, Eleftheria Girousi, Takashi K. Satoh, Barbara Meier-Schiesser, Lajos Kemény, Márta Széll, W. Wei-Lynn Wong, Steve Pascolo, Lars E. French, Thomas M. Kündig, Mark Mellett
Pubblicato in: Cell Death & Disease, Numero 16, 2025, ISSN 2041-4889
Editore: Nature Publishing Group
DOI: 10.1038/s41419-025-07537-9

Synthetic mRNAs Containing Minimalistic Untranslated Regions Are Highly Functional In Vitro and In Vivo

Autori: Shahab Mamaghani, Rocco Roberto Penna, Julia Frei, Conrad Wyss, Mark Mellett, Thomas Look, Tobias Weiss, Emmanuella Guenova, Thomas M. Kündig, Severin Lauchli, Steve Pascolo
Pubblicato in: Cells, Numero 13, 2024, Pagina/e 1242, ISSN 2073-4409
Editore: MDPI AG
DOI: 10.3390/cells13151242

Lipofection with Synthetic mRNA as a Simple Method for T-Cell Immunomonitoring

Autori: Jarzebska NT, Frei J, Lauchli S, French LE, Guenova E, Gouttefangeas C, Kündig TM, Mellett M, Pascolo S
Pubblicato in: Viruses, Numero 13, 2021, Pagina/e 1232, ISSN 1999-4915
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13071232

Data-driven protease engineering by DNA-recording and epistasis-aware machine learning

Autori: Lukas Huber, Tim Kucera, Simon Höllerer, Karsten Borgwardt, Sven Panke, Markus Jeschek
Pubblicato in: Nature Communications, Numero 16, 2025, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-025-60622-7

CRISPR-Cas9 recognition of enzymatically synthesized base-modified nucleic acids.

Autori: Yang H, Eremeeva E, Abramov M, Jacquemyn M, Groaz E, Daelemans D, Herdewijn P.
Pubblicato in: Nucleic Acids Research, 2023, ISSN 0305-1048
Editore: Oxford University Press
DOI: 10.1093/nar/gkac1147

Vaccines against COVID-19: Priority to mRNA-Based Formulations.

Autori: Pascolo S
Pubblicato in: Cells, Numero 10, 2021, Pagina/e 2716, ISSN 2073-4409
Editore: MDPI
DOI: 10.3390/cells10102716

Protamine-Based Strategies for RNA Transfection

Autori: Jarzebska NT, Mellett M, Frei J, Kündig TM, Pascolo S
Pubblicato in: Pharmaceutics, Numero 13, 2021, Pagina/e 877, ISSN 1999-4923
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13060877

Nonreplicating synthetic mRNA vaccines: A journey through the European (Journal of Immunology) history

Autori: Pascolo S
Pubblicato in: Europ. J. Immun., 2023, ISSN 0014-2980
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/eji.202249941

Differential functionality of fluoropyrimidine nucleosides for safe cancer therapy

Autori: Tim Holzinger, Julia Frei, Natalia Teresa Jarzebska, Hans-Dietmar Beer, Thomas M. Kündig, Steve Pascolo, Severin Läuchli, Mark Mellett
Pubblicato in: Anti-Cancer Drugs, Numero 35, 2025, Pagina/e 912-921, ISSN 0959-4973
Editore: Lippincott Williams & Wilkins Ltd.
DOI: 10.1097/cad.0000000000001644

Implications of mRNA-based SARS-CoV-2 vaccination for cancer patients

Autori: Romano E, Pascolo S, Ott P
Pubblicato in: J Immunother Cancer, Numero 9, 2021, Pagina/e e002932, ISSN 2051-1426
Editore: BMJ
DOI: 10.1136/jitc-2021-002932

Modulation of the tetrameric I-motif folding of C-rich Tetrahymena telomeric sequences by hexitol nucleic acid (HNA) modifications.

Autori: Ghezzo M, Grigoletto L, Rigo R, Herdewijn P, Groaz E, Sissi C.
Pubblicato in: Biochimie, 2023, ISSN 0300-9084
Editore: Elsevier BV
DOI: 10.1016/j.biochi.2023.08.003