Synthetic Biology of mRNA

Informacje na temat projektu

NEWmRNA

Identyfikator umowy o grant: 965135

DOI

10.3030/965135

Projekt został zamknięty

Data podpisania przez KE 16 Grudnia 2020

Data rozpoczęcia 1 Marca 2021

Data zakończenia 31 Sierpnia 2025

Finansowanie w ramach

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Koszt całkowity

€ 2 998 545,00

Wkład UE

€ 2 998 545,00

Koordynowany przez

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland

CORDIS oferuje możliwość skorzystania z odnośników do publicznie dostępnych publikacji i rezultatów projektów realizowanych w ramach programów ramowych HORYZONT.

Odnośniki do rezultatów i publikacji związanych z poszczególnymi projektami 7PR, a także odnośniki do niektórych konkretnych kategorii wyników, takich jak zbiory danych i oprogramowanie, są dynamicznie pobierane z systemu OpenAIRE .

Rezultaty

Documents, reports (17)

Synthesis of novel nucleoside triphosphates, part I

Report on nonconfidential synthesis of novel nucleoside triphosphates with modified bases focus on pyrimidinelike bases

Partially operative T7 RNAPol variant

Report on nonconfidential aspects of first partially operative T7 RNAPol variants

Summary characterization of T7 RNAPol variants

Report on non-confidential aspects of the different T7 RNAPol variants obtained over the project

Synthesis of novel nucleoside triphosphates for in vitro transcription

Report on nonconfidential aspects of synthesis of novel nucleoside triphosphates with noncanonical sugars for in vitro transcription U and Ccarrying bases

Optimized CNA synthesis

Summary report on non-confidential aspects of the optimization of in vivo CNA synthesis

Dissemination and exploitation plan
Chemical assembly of short mRNAs, part I

Report on nonconfidential aspects of chemical assembly of short mRNAs

Production of circmRNA in E. coli (long mRNA)

Protocol for efficient production of (long) circmRNA production in E. coli.

Report on .eu project website and logo

Report on eu project website and logo M2 ETH Zrich

Enzyme with hydrolytic activity for cNMP

Summary report on nonconfidential aspects of enzyme development for deblocking cNMP

Report on NEWmRNA summer school
CNA precursor uptake

Report on nonconfidential aspects of import engineering for CNA precursors

Robust E. coli chassis lacking phosphoribose and PRPP metabolism

Summary report on nonconfidential aspects of engineering PRPP metabolismfree E coli

Production of circRNA in E. coli (short RNA)

Protocol for efficient production of short circRNA production in E coli

Synthesis of transport and in vivo transcription substrates, part I

Report on nonconfidential aspects of synthesis of transport and in vivo transcription substrates using carbamate cNTPs focus on U and Cbases

Establishment of T7 RNAPol mutant libraries and HTP screen

Report on the establishment of T7 RNAPol mutant libraries and a suitable HTP screen

Characterization of in vivo functionality of oligonucleotides, part I

Preliminary summary report on nonconfidential aspects of the characterization of in vivo functionality of oligonucleotides with noncanonical pyrimidine or purine base moieties

Open Research Data Pilot (1)

Data management plan

Report on data management plan

Publikacje

Peer reviewed articles (14)

RNA with chemotherapeutic base analogues as a dual-functional anti-cancer drug

Autorzy: Natalia Teresa Jarzebska, Marina Tusup, Julia Frei, Tobias Weiss, Tim Holzinger, Mark Mellett, Mustafa Diken, Simon Bredl, Michael Weller, Roberto F. Speck, Thomas M. Kündig, Ugur Sahin, Steve Pascolo
Opublikowane w: OncoImmunology, Numer 11, 2025, ISSN 2162-402X
Wydawca: Informa UK Limited
DOI: 10.1080/2162402x.2022.2147665

A microbiological system for screening the interference of XNA monomers with DNA and RNA metabolism.

Autorzy: Blanchard, A., Abramov, M., Hassan, C., Marliere, P., Herdewijn, P., Pezo, V. A
Opublikowane w: RSC Advances, 2023, ISSN 2046-2069
Wydawca: Royal Society of Chemistry
DOI: 10.1039/d3ra06172h

In vivo assembly and expression of DNA containing non-canonical bases in the yeast Saccharomyces cerevisiae.

Autorzy: Nguyen, H., Abramov, M., Rozenski, J., Ermeeva, E., Herdewijn, P.
Opublikowane w: ChemBioChem, 2023, ISSN 1439-4227
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202200060

LL37 complexed to double-stranded RNA induces RIG-I-like receptor signalling and Gasdermin E activation facilitating IL-36γ release from keratinocytes

Autorzy: Jennifer Keller, Judit Danis, Isabella Krehl, Eleftheria Girousi, Takashi K. Satoh, Barbara Meier-Schiesser, Lajos Kemény, Márta Széll, W. Wei-Lynn Wong, Steve Pascolo, Lars E. French, Thomas M. Kündig, Mark Mellett
Opublikowane w: Cell Death & Disease, Numer 16, 2025, ISSN 2041-4889
Wydawca: Nature Publishing Group
DOI: 10.1038/s41419-025-07537-9

Synthetic mRNAs Containing Minimalistic Untranslated Regions Are Highly Functional In Vitro and In Vivo

Autorzy: Shahab Mamaghani, Rocco Roberto Penna, Julia Frei, Conrad Wyss, Mark Mellett, Thomas Look, Tobias Weiss, Emmanuella Guenova, Thomas M. Kündig, Severin Lauchli, Steve Pascolo
Opublikowane w: Cells, Numer 13, 2024, Strona(/y) 1242, ISSN 2073-4409
Wydawca: MDPI AG
DOI: 10.3390/cells13151242

Lipofection with Synthetic mRNA as a Simple Method for T-Cell Immunomonitoring

Autorzy: Jarzebska NT, Frei J, Lauchli S, French LE, Guenova E, Gouttefangeas C, Kündig TM, Mellett M, Pascolo S
Opublikowane w: Viruses, Numer 13, 2021, Strona(/y) 1232, ISSN 1999-4915
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/v13071232

Data-driven protease engineering by DNA-recording and epistasis-aware machine learning

Autorzy: Lukas Huber, Tim Kucera, Simon Höllerer, Karsten Borgwardt, Sven Panke, Markus Jeschek
Opublikowane w: Nature Communications, Numer 16, 2025, ISSN 2041-1723
Wydawca: Nature Publishing Group
DOI: 10.1038/s41467-025-60622-7

CRISPR-Cas9 recognition of enzymatically synthesized base-modified nucleic acids.

Autorzy: Yang H, Eremeeva E, Abramov M, Jacquemyn M, Groaz E, Daelemans D, Herdewijn P.
Opublikowane w: Nucleic Acids Research, 2023, ISSN 0305-1048
Wydawca: Oxford University Press
DOI: 10.1093/nar/gkac1147

Vaccines against COVID-19: Priority to mRNA-Based Formulations.

Autorzy: Pascolo S
Opublikowane w: Cells, Numer 10, 2021, Strona(/y) 2716, ISSN 2073-4409
Wydawca: MDPI
DOI: 10.3390/cells10102716

Protamine-Based Strategies for RNA Transfection

Autorzy: Jarzebska NT, Mellett M, Frei J, Kündig TM, Pascolo S
Opublikowane w: Pharmaceutics, Numer 13, 2021, Strona(/y) 877, ISSN 1999-4923
Wydawca: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13060877

Nonreplicating synthetic mRNA vaccines: A journey through the European (Journal of Immunology) history

Autorzy: Pascolo S
Opublikowane w: Europ. J. Immun., 2023, ISSN 0014-2980
Wydawca: John Wiley & Sons Ltd.
DOI: 10.1002/eji.202249941

Differential functionality of fluoropyrimidine nucleosides for safe cancer therapy

Autorzy: Tim Holzinger, Julia Frei, Natalia Teresa Jarzebska, Hans-Dietmar Beer, Thomas M. Kündig, Steve Pascolo, Severin Läuchli, Mark Mellett
Opublikowane w: Anti-Cancer Drugs, Numer 35, 2025, Strona(/y) 912-921, ISSN 0959-4973
Wydawca: Lippincott Williams & Wilkins Ltd.
DOI: 10.1097/cad.0000000000001644

Implications of mRNA-based SARS-CoV-2 vaccination for cancer patients

Autorzy: Romano E, Pascolo S, Ott P
Opublikowane w: J Immunother Cancer, Numer 9, 2021, Strona(/y) e002932, ISSN 2051-1426
Wydawca: BMJ
DOI: 10.1136/jitc-2021-002932

Modulation of the tetrameric I-motif folding of C-rich Tetrahymena telomeric sequences by hexitol nucleic acid (HNA) modifications.

Autorzy: Ghezzo M, Grigoletto L, Rigo R, Herdewijn P, Groaz E, Sissi C.
Opublikowane w: Biochimie, 2023, ISSN 0300-9084
Wydawca: Elsevier BV
DOI: 10.1016/j.biochi.2023.08.003