Skip to main content
Un sito ufficiale dell’Unione europeaUn sito ufficiale dell’UE
European Commission logo
italiano italiano
CORDIS - Risultati della ricerca dell’UE
CORDIS
CORDIS Web 30th anniversary CORDIS Web 30th anniversary
Contenuto archiviato il 2024-04-30

Three-dimensional solution structure of monomeric superoxide dismutase and its mutants - structure-function correlations

Obiettivo



The three-dimensional structure in solution of a monomeric form of the Cu(II)-Co(II) containing superoxide dismutase (SOD) will be determined by using multidimensional NMR spectroscopy. Previous biophysical studies have shown that residues Arg143 and Thr137, located close to the Cu(II) site, play an important role in the reaction catalyzed by the enzyme. Site-specific mutations at these two positions will be carried out, and the solution structure of these two SOD derivatives will also be determined. Through the detailed comparison and analysis of the changes induced in the structure and dynamics of the active site channel upon mutation, correlations will be established between structure and enzymatic function. Due to the paramagnetism of the protein modifications to the standard NMR methodologies as well as development of new ones will be required in order to acquire a complete sequence-specific signal assignment and thus successfully proceed to the solution structure determination of monomeric CuCoSOD and its mutants.

Campo scientifico

CORDIS classifica i progetti con EuroSciVoc, una tassonomia multilingue dei campi scientifici, attraverso un processo semi-automatico basato su tecniche NLP.

Invito a presentare proposte

Data not available

Coordinatore

UNIVERSITY OF FLORENCE
Contributo UE
Nessun dato
Indirizzo
Via Gino Capponi 7
50121 Firenze
Italia

Mostra sulla mappa

Costo totale
Nessun dato

Partecipanti (1)