Analysis of developmental expression pattern of xenopus laevis genes

Projektinformationen

ID Finanzhilfevereinbarung: FMBI961766

Startdatum 1 Februar 1997

Enddatum 31 Januar 1999

Finanziert unter

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Gesamtkosten € 0,00

EU-Beitrag Keine Daten

Koordiniert durch

Stiftung Deutsches Krebsforschungszentrum
Germany

Ziel

Two basic steps in the investigation of normal development are the identification of regulatory genes and the characterisation c their expression patterns, the latter being are a prerequisite for functional studies. Expression pattern of related genes in different vertebrate species have proven predictable power for other vertebrate species, including man, where gene expression analyses are not easily feasible. We propose to use Xenopus embryo as a vertebrate model system to systematically study gene expression. Our objectives are
? to carry out a molecular screen by in situ hybridisation in Xenopus embryos. documenting and characterizing the expression c 10 000 embryonal expressed mRNAs
? to tag sequence the corresponding cDNAs to match expression patterns to genes and identify genes wlth a potential function i growth control and differentiation
? to establish a Xenopus embryonic gene expression database and install it on the Internet
Training content (objective, benefit and expected impact)
The completion of this research project will train me in various fields. I will gain a general knowledge about development biology problematics and methods: anatomy, concepts on developmental processes... I count on having a more specific knowledge of the Xenopus model and of gene patterning in embryos. I expect that the analysis of the gene patterns will give new and important clues to understand some processes of normal development, in particular mesoderm regionalisation. The us of a vertebrate model of development is of particular interest due to its validity for human development, normal or pathologic The construction and management of the database will give me the opportunity to interact with bioinformatics scientists, and with researchers engaged in similar projects in other organisms (mouse, zebrafish...).
On the whole, my expected training should give me the possibility thereafter to do developmental biology, and to be able tS investigate the functions of genes during vertebrate embryogenesis, including human. I expect to better control bioinformatric to manage and interpret biological data. Given the exponential accumulation of data this should give me the opportunity thereafter to address biological problems using multidisciplinary approaches where bioinformatics, developmental biology, comparative analysis and genome approach play a role.

Wissenschaftliches Gebiet

• natural sciences › computer and information sciences › internet
• natural sciences › computer and information sciences › databases
• natural sciences › biological sciences › developmental biology
• medical and health sciences › clinical medicine › embryology
• natural sciences › biological sciences › genetics › genomes

Programm/Programme

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Thema/Themen

• 0302 - Post-doctoral research training grants
• TL06 - Developmental Biology

Aufforderung zur Vorschlagseinreichung

Data not available

Finanzierungsplan

RGI - Research grants (individual fellowships)

Koordinator

Stiftung Deutsches Krebsforschungszentrum

• EU-Beitrag: Keine Daten
• Adresse: 280,Im Neuenheimer Feld ; 69120 Heidelberg ; Deutschland
• Gesamtkosten: Keine Daten

Beteiligte (1)

Not available

Frankreich

• EU-Beitrag: Keine Daten
• Gesamtkosten: Keine Daten