Objective
The research topic deals with the structure-function relationship of the RT of the HIV of type 1 and 2, several mutant forms of HIV-1 RT and integrase. R T is crucial in the replication cycle of the retrovirus: the role of RT is to synthesize the double stranded proviral DNA from the retroviral RNA genome. The proviral DNA is then integrated into the host nuclear genome in a reaction catalyzed by viral integrase (IN). Both enzymes are the main targets of the therapeutical treatment of AIDS.
Recently we have developed new kinetic and thermodynamic approaches for analysis of protein-nucleic acids recognition, which make it possible to reveal the individual nucleotide unit contribution to the single and double-stranded nucleic acids interactions with the recognizing enzymes. Structural analysis of HIV-1 RT was done by methods of crosslinking and highly selective affinity modification and other different physico-chemical approaches. A detailed analysis of the mechanism of various natural substrate recognition by RT and its mutant form as well as by integrase using various methods will be done. Better understanding of the biochemical mechanism of action of the enzymes and of the difference in functioning of RT mutant forms may help us to design new drugs with higher inhibitory activity as well as with decreased potential for resistance. Inhibitor capacity of nucleotide and oligonucleotide containing several new organic compounds as well as new non-natural oligopepetides interacting with small grove of DNA and stabilizing interaction of specific sequence strands will be used.
According to our previous data HIV integrase most probably possess the additional enzymatic activities: it is capable to convert ATP to unusual products like ppAppA, ppA pppA an so on, and to self-phosphorylate in the presence of ATP. The detailed analysis of the mechanism of integrase functioning in order to establish the proposed functions is planed.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project has not yet been classified with EuroSciVoc.
Be the first one to suggest relevant scientific fields and help us improve our classification service
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Data not available
Coordinator
33077 Bordeaux
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.