Natural apomixis as a novel tool in plant breeding

Informazioni relative al progetto

APOTOOL

ID dell’accordo di sovvenzione: QLG2-CT-2000-00603

Progetto chiuso

Data di avvio 1 Gennaio 2001

Data di completamento 30 Giugno 2004

Finanziato da

• Specific Programme for research, technological development and demonstration on "Quality of life and management of living resources", 1998-2002

Costo totale

€ 3 006 969,00

Contributo UE

€ 1 895 847,00

Coordinato da

WAGENINGEN UNIVERSITY
Netherlands

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Risultati finali

SSLP based procedure to detect rare apomicts in a sexual population

To screen for putative apomictic embryos possibly due to introduction of a key gene into a fully functional sexual background the Single Sequence Length Polymorphism (SSLP) assay was used to detect plants heterozygous for up to 15 markers. F2 populations of AtDMC1: AtSERK1 overexpressing plants generated during year 1 and 2 were screened for putative apomictic seeds using the SSLP analysis. The population showed segregation pattern similar to the wt. The highest number of heterozygous SSLP markers in a single plant found in this population was 15 and the chance to get this plant by chance only was more then 1/4000. Thus the procedure is sensitive enough to find as low is 0.1 % of potential apomicts within an otherwise sexually propagated population of plants.

Optimized Poa pratensis transformation system for the manipulation of gene expression, used successfully with apomictic germplasm

A major objective of WP6 is to deliver novel germplasm of Poa pratensis expressing newly identified candidate genes derived in WP1. Using methodology developed in Year 1, a number of different P.pratensis germplasm provided by partner 3 were screened for responsiveness in tissue culture. Stable transformants of P.pratensis have been generated, using germplasm A24 and test constructs expressing uidA, and the presence of the introduced transgenes confirmed in T0 transformants. To complement transformation of apomictic P.pratensis, a number of different sexual P. pratensis were tested for responsiveness to tissue culture; in all germplasm screened responsivenes of sexual P.pratensis was low and stable transformation could not be detected. The most consistently responsive sexual germplasm identified were derivatives of the S3 line. Transition to reproductive growth appears to be strongly inhibited in P.pratensis that has undergone regeneration through tissue culture. Plants will continue to be examined for flowering after vernalisation treatments in subsequent years as part of the continued analysis of this material after the end of ApoTool.