Ziel
Measurement of the enzyme prostatic acid phosphatase (PAP) in serum is an established procedure in monitoring metastatic carcinoma of the prostate and is also useful in confirming suspected early stage disease.
The project aims at preparing purified acid phosphatase form human prostate. The project also includes the investigation and subsequent development of an assay method of sufficient reliability and robustness for assigning a value to the PAP concentration in the enzyme material.
RESULTS
The purification of acid phosphatase from human prostatic tissue has been successful. A batch of 3000 samples in lyophilised form was prepared for certification. The method used was the one with 4-nitrophenyl phosphate as substrate and measurements were carried out at 37 C. 9 laboratories were involved and the following value and its uncertainty were proposed for certification:
CRM 410 (28.0 +/- 0.7) U/l.
The report describing the preparation and the certification exercise is being finalized for publication.
Wissenschaftliches Gebiet
Programm/Programme
Thema/Themen
Data not availableAufforderung zur Vorschlagseinreichung
Data not availableFinanzierungsplan
CSC - Cost-sharing contractsKoordinator
W12 ONN London
Vereinigtes Königreich