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AFLATOXIN B1 IN EDIBLE PEANUTS RMs

Objective


Aflatoxins are potent carcinogens and may occur as a consequence of fungal attack in certain food. There is therefore extensive monitoring by food control laboratories and by the food industry to ensure that vulnerable products such as peanuts and figs satisfy national requirements. Most member states impose limits of either 5 ug/kg of aflatoxin B1 or 10 ug/kg of total aflatoxin (B1+B2+G1+G2). Immunological assays are increasingly used for rapid screening and commercial kits require validation by means of reference materials (RM). Two peanut butter RMs were certified for their individual and total aflatoxin contents. The certified contents in ug/kg are as follows:
RM number 385: B1 = 7.0(+/-0.8); B2 = 1.1(+/-0.2); G1 = 1.7(+/-0.3); G2 = 0.3(+/-0.1); Total = 10.1(+/-0.9);
RM number 401: B1 < 0.2; B2 < 0.1; G1 < 0.1; G2 < 0.1; Total < 0.1.
Certification measurements were made by 9 laboratories using a variety of extraction and clean up procedures with high performance liquid chromatography (HPLC) as the final step.
Aflatoxins are potent carcinogens and may occur as a consequence of fungal attack in certain food. There is therefore extensive monitoring by food control laboratories and by the food industry to ensure that vulnerable products such as peanuts and figs satisfy national requirements. Most Member States impose limits of either 5 ug/kg of aflatoxin B1 or 10 ug/kg of total aflatoxin (B1+B2+G1+G2). Immunological assays are increasingly used for rapid screening and commercial kits require validation by means of reference materials. The objective of the project was to provide appropriate CRMs.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

MAFF Food Science Laboratory
Address
Norwich Research Park Colney
NR4 7UQ Norwich
United Kingdom

Participants (9)

Istituto Superiore della Sanità
Italy
Address
Viale Regina Elena 299
00161 Roma
Keuringsdienst van Waren voor het Gebied Rotterdam
Netherlands
Address
74,Baan
3011 CD Rotterdam
Laboratoire Interregional de la Répression des Fraudes
France
Address
2 Rue Saint Pierre
34024 Montpellier
Laboratoire Interrégional de la Répression des Fraudes
France
Address
25 Avenue De La République
91305 Massy
Laboratory of the Government Chemist (LGC)
United Kingdom
Address
Queen's Road
TW11 0LY Teddington
Rank Hovis McDougall Technology Ltd
United Kingdom
Address
Lord Rank Centre Lincoln Road
HP12 3QR High Wycombe
Rijksinstituut voor Volksgezondheid en Milieuhygiene
Netherlands
Address

3720 BA Bilthoven
Royal Veterinary and Agricultural University
Denmark
Address
12,Bülowsvej
1870 Copenhagen V
TNO Biotechnology & Chemistry Institute
Netherlands
Address

3700 AJ Zeist