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IMPROVEMENT IN THE DETERMINATION OF OCHRATOXIN-A IN FARM ANIMALS, PRODUCTS AND CEREALS

Objective


Ochratoxin A (OA), a mycotoxin, is a suspect kidney carcinogen and has been shown to have teratogenic activity. Its occurrence as a consequence of fungal attack in foods from plant origin such as cereals and beans is well known and widely reported in the scientific literature. The presence of OA in edible animal tissues and particularly in pig kidneys, as a result of consumption of contaminated feed has been demonstrated in Denmark and Sweden. Recently, individual member states have begun to set up tolerance limits. A guideline for cereals exists in France and in the United Kingdom and industrial limit for cereals is used. The project was set up to improve the reliability of the methods to determine OA and to provide blank and naturally contaminated wheat and lyophilised pig kidney reference materials (RM).

A pair of wheat reference materials have been prepared. They consist of naturally contaminated samples containing the toxin at levels close to the recommended (voluntary) action limits, and samples having low levels to provide a means of checking analytical background and recovery efficiency. An intercomparison study with about 30 European laboratories was carried out and the results showed that the major difficulties arise from the clean up step of the method used.
Ochratoxin A (OA), a mycotoxin, is a suspect kidney carcinogen and has been shown to have teratogenic activity.
Its occurrence as a consequence of fungal attack in foods from plant origin such as cereals and beans is well known and widely reported in the scientific literature. The presence of OA in edible animal tissues and particularly in pig kidneys, as a result of consumption of contaminated feed has been demonstrated in Denmark and Sweden.
Recently, individual Member States have begun to set up tolerance limits. A guideline for cereals exists in France and in the UK an industrial limit for cereals is used.
The objective of the project is to improve the reliability of the methods to determine OA and to provide "blank" and naturally contaminated wheat and lyophilised pig kidney RMs.

Coordinator

Royal Veterinary and Agricultural University
Address
12,Bülowsvej
1870 Copenhagen V
Denmark

Participants (28)

Bayerische Landesanstalt für Tierzucht
Germany
Address
Prof Dürrwächter Platz 1
8011 Grub
Biocode Ltd
United Kingdom
Address
University Road Heslington
YO1 5DE York
Centro de Investigacion y Control de Calidad
Spain
Address
Avda De Cantabria S/n
28042 Madrid
DANISH MEAT RESEARCH INSTITUTE
Denmark
Address
2,Maglegaardsvej 2
4000 Roskilde
Istituto Superiore della Sanità
Italy
Address
Viale Regina Elena 299
00161 Roma
Istituto Tossine e Micotossine da Parassiti Vegetali
Italy
Address
Via G Amendola 197/F
70126 Bari
Laboratoire Interrégional du DGCCRF
France
Address
26 Avenue Du Coëtlogon
35000 Rennes
Laboratorio Nacional de Engenharia e Technologia Industrial
Portugal
Address
1,Rua Vale Formoso
1900 Lisboa
Laboratory of the Government Chemist (LGC)
United Kingdom
Address
Queen's Road
TW11 0LY Teddington
Landeshauptstadt Stuttgart
Germany
Address
Staffelbergstraße 81
70184 Stuttgart
MAFF Food Science Laboratory
United Kingdom
Address
Norwich Research Park Colney
NR4 7UQ Norwich
Max-von-Pettenkofer-Institut
Germany
Address
Thielallee 88-92
14195 Berlin
Ministerio de Sanidad y Consumo
Spain
Address
Carretera Majadahonda - Pozuelo, Km 2,2
28220 Majadahonda (Madrid)
National Food Agency of Denmark
Denmark
Address
19,Mörkhöj Bygade
2860 Söborg
National Laboratory for Agricultural Chemistry of Sweden
Sweden
Address
Fodersektionen
75007 Uppsala
National Veterinary and Food Research Institute of Helsinki
Finland
Address

00101 Helsinki
RIKILT
Netherlands
Address

6700 AE Wageningen
Rank Hovis McDougall Technology Ltd
United Kingdom
Address
Lord Rank Centre Lincoln Road
HP12 3QR High Wycombe
Rhône-Poulenc Diagnostics
United Kingdom
Address
Montrose House 187 George Street
G4 1YT Glasgow
Rijksinstituut voor Volksgezondheid en Milieuhygiene
Netherlands
Address

3720 BA Bilthoven
Royal Institute of Technology
Sweden
Address

10044 Stockholm
State Laboratory
Ireland
Address
Abbotstown Castleknock
15 Dublin
Statens Livsmedelsverk
Sweden
Address
Hamnesplanaden 5
75126 Uppsala
TNO Biotechnology & Chemistry Institute
Netherlands
Address

3700 AJ Zeist
Unilever UK Central Resources Ltd
United Kingdom
Address
Colworth House
MK44 1LQ Sharnbrook
Universidad Complutense
Spain
Address

28040 Madrid
University of Utrecht
Netherlands
Address

3508 TD Utrecht
Universität München
Germany
Address
Veterinärstraße 13
80539 München