Cel The aim of the project is to produce deoxyribonucleic acid (DNA) probes that reveal high levels of variability at single loci of the sea bream, Sparus aurata, the African catfish, Clarias gariepinus, and the sea bass, Dicentrachus labrax. The probes will make possible the identification of fish for genetic breeding and for the production of stocks suitable for aquaculture. Research is being carried out in order to produce deoxyribonucleic acid (DNA) probes that reveal high levels of variability at single loci of the gilthead sea bream Sparus aurata, the African catfish Clarias gariepinus and the sea bass Dicentrachus labrax. The probes will make possible the identification of fish for genetic breeding and for the production of stocks suitable for aquaculture.In the sea bream several highly variable microsatellite loci have been developed and are being used in a breeding experiment designed to identify parents that produce fast growing progeny. About 30 adults have been spawned and the resulting progeny have been grown together in 1 pool. Blood was removed for DNA extraction from parents and a random sample of about 150 progeny drawn from the common pool at 2 periods, at the age of 7 and 10 months. These offspring were weighed. The use of DNA probes will make it possible to identify the parents of each offspring. This information will be used to search for statistical associations between growth rates among full sibs, half sibs and unrelated progeny. Parents whose progeny grew faster will be identified and can be used subsequently as exclusive parents for the production of a new set progeny.The application of the probes in the sea bream breeding experiments is in progress in the Institute of Marine Biology of Crete, which maintains a large programme of research in sea bream aquaculture. Part of an ongoing research programme in catfish, will produce and evaluate the importance of gynogenetic stocks for aquaculture. Microsatellite probes developed by this research will be used to screen for gynogenetic fish.Specifically, DNA and microsatellite or minisatellite probes will be isolated for the detection of fragment size polymorphisms which will be tested for their Mendelian inheritance and for their ability to provide means of identification of individual fish and for parent offspring matching in breeding experiments. In the use of catfish, the probes will also be used to confirm the gynogenetic status of fish produced in laboratory experiments. For the sea bream in particular, the probes will be used in a breeding experiment designed to identify parents that produce fast growing progeny. About 30 adults (of which about half will be males and half females) will be mass spawned and the resulting progeny will be grown together in one pool. Blood will be removed from each parent and DNA extracted. The DNA will be stored for subsequent scoring of genotypes when the probes become available. Random samples of about 150 progeny will be drawn from the common pool at 2 periods, at the age of 7 and 10 months. These offspring will be weighed and their DNA extracted for genotyping. The use of DNA probes will make it possible to identify the parents of each offspring. This information will be used to search for statistical associations between growth rates (weight, the age of 7 or 10 months) among full sibs, half sibs and unrelated progeny. Parents whose progeny grew faster will be identified and can be used subsequently as exclusive parents for the productio of a new set of progeny. The development of genetic probes will be done by all contractors. Contractor 1 will undertake the responsibility for the probes of the sea bream and also for the first phase of development of probes for the catfish. Contractor 2 will be responsible for the first phase of development of probes for sea bass. The application of the probes in the sea bream breeding experiment will be done by contractor 1 in the Institute of Marine Biology of Crete which maintains a large programme of research on sea bream aquaculture. Contractor 3 has an ongoing research programme on catfish, part of which is to produce and evaluate the importance of gynogenetic stocks for aquaculture. The probes will be used to screen for gynogenetic fish. Dziedzina nauki natural sciencesbiological sciencesmarine biologyagricultural sciencesagriculture, forestry, and fisheriesfisheriesnatural sciencesbiological sciencesgeneticsDNA Program(-y) FP2-FAR - Community research and coordination programmes (EEC) in the fisheries sector, 1988-1992 Temat(-y) Data not available Zaproszenie do składania wniosków Data not available System finansowania Data not available Koordynator Institute of Marine Biology of Crete Wkład UE Brak danych Adres PO Box 2214 71003 Iraklion - Crete Grecja Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (2) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko UNIV LEUVEN (KUL)-ZOOL INST-LAB ECOLOGIE AQUACULTURE Belgia Wkład UE Brak danych Adres Leuven Zobacz na mapie Koszt całkowity Brak danych Université de Montpellier II (Université des Sciences et Techniques du Languedoc) Francja Wkład UE Brak danych Adres Quai de la Daurade 34200 Sète Zobacz na mapie Koszt całkowity Brak danych
