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Outer Membrane Vesicles (OMVs) from “Vaccinobacter”: A Synthetic Biology approach for effective vaccines against infectious diseases and cancer

Objective

This proposal intends to apply Synthetic Biology to create a new bacterial species, Vaccinobacter, devoted to the production of multivalent, highly effective vaccines. The project originates from the evidence that Outer membrane Vesicles (OMVs) naturally produced by all Gram-negative bacteria can induce remarkable protective immunity, a property already exploited to develop anti-Neisseria vaccines now available for human use. OMV protection is mediated by the abundance of Pathogen-Associated-Molecular Patterns (PAMPs), known to play a key role in stimulating innate immunity. Moreover, OMVs can be engineered by delivering recombinant proteins to bacterial periplasm and outer membrane. Intrinsic adjuvanticity and propensity to be manipulated potentially make OMVs an ideal vaccine platform, particularly indicated when antigen combinations (for pathogens with genetic variability) and strong potentiation of immunity (for the elderly and cancer) are needed. However, full exploitation of OMVs as vaccines is prevented by: i) presence of potentially reactogenic compounds such as LPS, virulence factors, and toxins, ii) presence of several irrelevant proteins, which dilute immune responses, iii) lack of broadly applicable molecular tools to load OMVs with foreign antigens. Scope of the project is to provide novel solutions to solve these limitations and demonstrate the unique performance OMVs as vaccines by testing them on complex pathogens and cancer. Main project activities are: 1) remodelling of E. coli genome to create “Vaccinobacter”, a “living factory” of OMVs deprived of all unnecessary components but carrying the relevant immune potentiators, 2) characterization and optimization of the immune stimulatory properties of OMVs, 3) development of novel methods to incorporate foreign antigens into Vaccinobacter-derived OMVs, 4) loading of OMVs with selected pathogen- and cancer-derived antigens and demonstration of their protective efficacy in appropriate animal models.

Field of science

  • /medical and health sciences/health sciences/infectious disease
  • /natural sciences/biological sciences/microbiology/bacteriology
  • /natural sciences/biological sciences/synthetic biology
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /medical and health sciences/clinical medicine/oncology/cancer
  • /medical and health sciences/basic medicine/pharmacology and pharmacy/pharmaceutical drug/vaccines

Call for proposal

ERC-2013-ADG
See other projects for this call

Funding Scheme

ERC-AG - ERC Advanced Grant

Host institution

UNIVERSITA DEGLI STUDI DI TRENTO
Address
Via Calepina 14
38122 Trento
Italy
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 859 840,07
Principal investigator
Guido Grandi (Dr.)
Administrative Contact
Vanessa Ravagni (Mrs.)

Beneficiaries (3)

UNIVERSITA DEGLI STUDI DI TRENTO
Italy
EU contribution
€ 1 859 840,07
Address
Via Calepina 14
38122 Trento
Activity type
Higher or Secondary Education Establishments
Principal investigator
Guido Grandi (Dr.)
Administrative Contact
Vanessa Ravagni (Mrs.)
EXTERNAUTICS SPA

Participation ended

Italy
EU contribution
€ 255 837,88
Address
Via Fiorentina 1
53100 Siena
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Paolo Sarmientos (Dr.)
FONDAZIONE TOSCANA LIFE SCIENCES
Italy
EU contribution
€ 497 150,05
Address
Via Fiorentina 1
53100 Siena
Activity type
Research Organisations
Administrative Contact
Ilaria Niccolai (Dr.)