Obiettivo The mechanistic target of rapamycin (mTOR) is a highly conserved serine/threonine kinase in eukaryotic organisms. mTOR is the catalytic subunit of two different complexes (mTORC1 and mTORC2), which differ by their subcellular localization and function. The mTORCs integrate the environmental and cellular signal and regulate a broad range of downstream growth events. Impaired regulation of mTOR was often observed in tumors, Alzheimer’s disease, several metabolic disorders, and is known to accelerate aging. Thus, the study of the mTOR pathway is of paramount importance for both fundamental biology and medicine. A tremendous amount of studies have been carried out to elucidate the regulatory role of mTOR in metabolism and diseases. Yet the detailed mechanism of the mTOR regulatory pathway remains to be completed. Recently, using the novel method “mRNA interactome capture”, the host laboratory identified proteins involved in the mTOR-signalling pathway that acts as RNA-binding proteins (RBPs) in yeast, mouse embryonic stem cells (mESC) and HeLa cells. This finding revealed a conserved and previously unrecognized function of the mTOR pathway with RNA being an important component of it. Here I propose a project aiming to unravel the biological function of RNAs and RBPs in the mTOR pathway. In this project, I will use the budding yeast Saccharomyces cerevisiae as a model system, and will identify the novel RNA binding domains (RBDs) of the RBPs. I will further investigate the network of the interacting RBPs and RNAs. This will elucidate the biological impact of the RNA-protein interactions in the mTOR pathway. I will carry out this interdisciplinary study using biochemical and genetic methods, as well as large-scale analysis. The datasets created in this study will open new perspectives on the mTOR regulatory pathway. Campo scientifico medical and health sciencesbasic medicineneurologydementiaalzheimernatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsmedical and health sciencesmedical biotechnologycells technologiesstem cellsnatural sciencesbiological sciencesgeneticsmutationnatural sciencesbiological sciencesgeneticsRNA Programma(i) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Argomento(i) MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Invito a presentare proposte H2020-MSCA-IF-2014 Vedi altri progetti per questo bando Meccanismo di finanziamento MSCA-IF-EF-ST - Standard EF Coordinatore EUROPEAN MOLECULAR BIOLOGY LABORATORY Contribution nette de l'UE € 171 460,80 Indirizzo Meyerhofstrasse 1 69117 Heidelberg Germania Mostra sulla mappa Regione Baden-Württemberg Karlsruhe Heidelberg, Stadtkreis Tipo di attività Research Organisations Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 171 460,80