Objective
Microvilli are tube-like membrane protrusions containing bundles of actin filaments located at the apical surface of epithelial cells. A key component of these microvilli is ezrin, a member of the Ezrin/Radixin/Moesin protein family, which provides a regulated linkage between the plasma membrane and the actin cytoskeleton. The critical role of ezrin in microvillar morphogenesis has been demonstrated. However, how ezrin is recruited to the apical surface of the cells and how ezrin contributes to the stability of microvillar morphogenesis remain elusive. Recently, a few ezrin binding partners, such as EBP50 and Eps8, have been identified and shown to function synergistically with ezrin in membrane and actin remodelling in cells. The precise mechanisms and implications of their interactions in microvillar morphogenesis are not well understood. Lately, I-BAR domain protein IRSp53 was found to colocalize with ezrin in microvilli and interact with both EBP50 and Eps8, as ezrin does. Given that I-BAR domain proteins participate in the formation of many cellular protrusions, such as filopodia, ezrin and I-BAR domain proteins may work synergistically in membrane remodelling.
The goal of this proposal is to better understand the interaction of ezrin, the membrane and actin, and the role of ezrin binding proteins and I-BAR domain proteins in this interaction by using reconstituted model systems based on purified proteins combined with supported lipid bilayers or giant unilamellar vesicles. In parallel, potential common binding partners of ezrin and I-BAR domain proteins will be identified, and their role in mediating the interaction of ezrin and I-BAR domain proteins on membranes will be studied. This project will thus lead to an improved understanding of the membrane and actin remodelling activity of ezrin, and advance our knowledge of cell and eventually tissue morphogenesis.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences biological sciences developmental biology
- natural sciences physical sciences optics microscopy
- natural sciences biological sciences biochemistry biomolecules lipids
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2014
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
75231 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.