Descrizione del progetto DEENESFRITPL Sensori di microRNA basati su nanoparticelle I biosensori sono dispositivi analitici che utilizzano componenti biologici come enzimi, anticorpi o acidi nucleici per rilevare e misurare sostanze specifiche nei campioni. Il componente biologico interagisce con l’analita target per produrre un segnale elettrico, ottico o elettrochimico misurabile. I biosensori hanno suscitato grande interesse per le applicazioni diagnostiche e terapeutiche. Finanziato dal programma azioni Marie Skłodowska-Curie, il progetto Upbiosens svilupperà un nuovo biosensore basato su nanoparticelle che incorpora filamenti di DNA per rilevare i microRNA, piccole molecole di RNA che fungono da regolatori dell’espressione genica. I ricercatori utilizzeranno i fluorofori per la misurazione quantitativa degli acidi nucleici, estendendo l’applicabilità di questi nuovi nanosensori alla terapia genica e all’analisi forense. Mostra l’obiettivo del progetto Nascondi l’obiettivo del progetto Obiettivo The Upbiosens project describes a novel method to produce water-dispersible and stable DNA-capped upconversion nanoparticles (UCNPs), keeping intact (or enhancing) the properties of both DNA and UCNPs. The UCNP@DNA nanohybrid will be able to detect the complementary target miRNA or DNA sequence. This detection will be based on the UCNP emission changes after NIR excitation in the presence of the fluorophore target RNA/DNA sequence. The hybridization will enable FRET from the UCNP to the fluorophore upon NIR irradiation, which will allow the quantitative measurement of nucleic acid. In addition, this new strategy for DNA functionalization of UCNPs will enable the control of the UCNP size during the functionalization step as well as the distance between UCNP and the energy acceptor. Both issues are critical for the design of novel, rapid, highly selective and sensitive FRET-based biosensors. This approach will be demonstrated for wo different compositions of UCNPs, NaYF4: Yb, Er (excitation at 980 nm) and NaYF4: Yb, Nd, Er (excitation at 800 nm) in order to enhance optical properties. The excitation at 800 nm reduces the overheating of biosamples due to the light absorption by water. Taking into account the exceptional UCNPs emissive properties, the capability for in vitro nucleic acid imaging of the nanobiosensor will also be evaluated. To our knowledge, the combination of a nucleic acid sensing and imaging using UCNP-based nanohybrids has not yet been attempted. This dual NIR NA biosensor/bioimaging nanohybrid can be promisingly bio-implemented, such as in prognosis, diagnostics and treatment of diseases, gene therapy, or forensic analysis. Campo scientifico engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringsensorsbiosensorsnatural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural sciencesbiological sciencesgeneticsDNAnatural sciencesbiological sciencesgeneticsRNAengineering and technologynanotechnologynano-materials Programma(i) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Argomento(i) MSCA-IF-2017 - Individual Fellowships Invito a presentare proposte H2020-MSCA-IF-2017 Vedi altri progetti per questo bando Meccanismo di finanziamento MSCA-IF-EF-ST - Standard EF Coordinatore CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS Contribution nette de l'UE € 185 076,00 Indirizzo RUE MICHEL ANGE 3 75794 Paris Francia Mostra sulla mappa Regione Ile-de-France Ile-de-France Paris Tipo di attività Research Organisations Collegamenti Contatta l’organizzazione Opens in new window Sito web Opens in new window Partecipazione a programmi di R&I dell'UE Opens in new window Rete di collaborazione HORIZON Opens in new window Costo totale € 185 076,00
