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Research empowerment on solute carriers (ReSOLUTE)

Deliverables

Data Management Plan 2

Initial version of DMP after 6 months, an updated version latest mid-term and a final version

Vector generation

Collection of lentiviral and Flp-In compatible vectors carrying CRISPR-based Cas9 and sgRNAs targeting all SLCs, together with vectors carrying tagged and untagged cDNAs for >80% of the SLC family

ReSOLUTE knowledgebase

Concept for database of integrated publicly available knowledge and ReSOLUTE web portal.

Data Mining

Data mining support for priority ranking of SLC family members for target selection in WP4.

SLC overexpression in HEK293

Collection of >300 HEK293 isogenic cell lines, each overexpressing a SLC gene, quality-controlled and characterized with a multi-parameter approach

Assays for SLCs based on fluorescent sensor proteins

A report summarizing results and protocols on measuring SLC-dependent metabolic states through fluorescent sensor proteins.

Assays for (electrogenic) transporters

A report summarizing results and protocols obtained using fluorescent sensitive dyes and impedance measurements.

Methodology for general detection of plasma metabolites and drug/dietary components

LC/GC-MS coupled to novel deconvolution routines for determining the approximately 3,000 molecules typically observable in serum

Data Management Plan 1

Initial version of DMP after 6 months, an updated version latest mid-term and a final version.

ReSOLUTE web portal

Data sharing and approval process, and prototype of interface for data access at public web portal up and running

ReSOLUTE database

Infrastructure and data warehouse for sharing annotated raw data files and analysis results; prototype ready after first year and annual releases of new versions.

Searching for OpenAIRE data...

Publications

An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters

Author(s): Vojtech Dvorak, Tabea Wiedmer, Alvaro Ingles-Prieto, Patrick Altermatt, Helena Batoulis, Felix Bärenz, Eckhard Bender, Daniela Digles, Franz Dürrenberger, Laura H. Heitman, Adriaan P. IJzerman, Douglas B. Kell, Stefanie Kickinger, Daniel Körzö, Philipp Leippe, Thomas Licher, Vania Manolova, Riccardo Rizzetto, Francesca Sassone, Lia Scarabottolo, Avner Schlessinger, Vanessa Schneider, Hubert J.
Published in: Frontiers in Pharmacology, Issue 12, 2021, ISSN 1663-9812
DOI: 10.3389/fphar.2021.722889

A substrate‐based ontology for human solute carriers

Author(s): Eva Meixner, Ulrich Goldmann, Vitaly Sedlyarov, Stefania Scorzoni, Manuele Rebsamen, Enrico Girardi, Giulio Superti‐Furga
Published in: Molecular Systems Biology, Issue 16/7, 2020, ISSN 1744-4292
DOI: 10.15252/msb.20209652

Label-free high-throughput screening assay for the identification of norepinephrine transporter (NET/SLC6A2) inhibitors

Author(s): Hubert J. Sijben, Wieke M. van Oostveen, Peter B. R. Hartog, Laura Stucchi, Andrea Rossignoli, Giovanna Maresca, Lia Scarabottolo, Adriaan P. IJzerman, Laura H. Heitman
Published in: Scientific Reports, Issue 11/1, 2021, ISSN 2045-2322
DOI: 10.1038/s41598-021-91700-7

Studies of structural determinants of substrate binding in the Creatine Transporter (CreaT, SLC6A8) using molecular models

Author(s): Claire Colas, Giulia Banci, Riccardo Martini, Gerhard F. Ecker
Published in: Scientific Reports, Issue 10/1, 2020, ISSN 2045-2322
DOI: 10.1038/s41598-020-63189-z

A study of the dopamine transporter using the TRACT assay, a novel in vitro tool for solute carrier drug discovery

Author(s): Hubert J. Sijben, Julie J. E. van den Berg, Jeremy D. Broekhuis, Adriaan P. IJzerman, Laura H. Heitman
Published in: Scientific Reports, Issue 11/1, 2021, ISSN 2045-2322
DOI: 10.1038/s41598-020-79218-w

The RESOLUTE consortium: unlocking SLC transporters for drug discovery

Author(s): Giulio Superti-Furga, Daniel Lackner, Tabea Wiedmer, Alvaro Ingles-Prieto, Barbara Barbosa, Enrico Girardi, Ulrich Goldmann, Bettina Gürtl, Kristaps Klavins, Christoph Klimek, Sabrina Lindinger, Eva Liñeiro-Retes, André C. Müller, Svenja Onstein, Gregor Redinger, Daniela Reil, Vitaly Sedlyarov, Gernot Wolf, Matthew Crawford, Robert Everley, David Hepworth, Shenping Liu, Stephen Noell, Mary Pio
Published in: Nature Reviews Drug Discovery, Issue 19/7, 2020, Page(s) 429-430, ISSN 1474-1776
DOI: 10.1038/d41573-020-00056-6