Project description
Ribosome heterogeneity during zebrafish embryogenesis
Embryogenesis involves coordinated changes in gene expression at transcriptional and translational levels. In zebrafish, translation at early stages of embryo development relies on maternal, oocyte-derived ribosomes which are completely replaced by newly synthesised somatic ribosomes within five days after fertilisation. The EU-funded RiboHeterogeneity project will investigate the regulatory potential of maternal and somatic ribosomes during zebrafish embryogenesis. The research will analyse the translational activities of maternal and somatic ribosomes, substrate specificity, mRNA sequence and length, the kinetics of translation and the regulation of ribosomal degradation during embryogenesis. The results of the project have the potential to reveal novel regulatory mechanisms of vertebrate embryogenesis and will shed light on the functional significance of heterogeneous ribosomes.
Objective
The process of embryogenesis involves coordinated changes in gene expression at both transcriptional and translational levels. In zebrafish, translation at early stages of embryo development relies on maternal, oocyte-derived ribosomes which are completely replaced by newly synthesized somatic ribosomes within 5 days after fertilization. Strikingly, preliminary data obtained in the Pauli lab suggest that maternal and somatic ribosomes diverge not only in rRNA content but also in protein composition and structure. Despite their heterogeneity, potential differences in the function of maternal and somatic ribosomes remain unknown. The dual ribosomal system present in zebrafish embryos thus provides a unique, powerful tool to address the controversial idea regarding the existence of ‘specialized ribosomes’ having distinct functions in translation.
In the proposed research project, I will investigate the regulatory potential of maternal and somatic ribosomes during zebrafish embryogenesis. To this end, I will analyze the translational activities of maternal and somatic ribosomes by determining substrate specificity, preferred mRNA features (sequence and length) and kinetics of translation. In addition, I will study the regulation of ribosomal degradation during embryogenesis in order to understand how and why the maternal ribosomal machinery is turned-over despite the high energy cost associated with making a new set of ribosomes. To accomplish these aims, I will combine diverse state-of-the-art methodologies, including in vivo and in vitro translation assays, biochemical ribosome purification techniques, SLAM-seq, and molecular and genetic studies in zebrafish. The results obtained have the potential to reveal novel regulatory mechanisms acting during vertebrate embryogenesis and will help to pinpoint the functional significance of heterogeneous ribosomes.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences developmental biology
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- medical and health sciences clinical medicine embryology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2019
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
1030 Wien
Austria
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.