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Negative-Ion ICP-MS as a Novel Strategy for Fluorine and Bromine Determination in Pharmaceutical Research

Project description

Safer analysis of pharmaceutical drugs

Understanding how drugs are absorbed, distributed and metabolised is essential for pharmaceutical development. Many drugs contain halogen atoms, such as fluorine or bromine, which serve as useful chemical tracers in these studies. However, current methods rely on radioactive labelling, which is costly, time-consuming, and generates hazardous waste. With the support of the Marie Skłodowska-Curie Actions programme, the PHARM-N-ICP project pioneers a radiolabel-free approach to detect and quantify halogens directly in biological samples. Researchers will develop validated methods for bulk quantification, metabolite profiling, and high-resolution tissue mapping of halogenated drugs. This analytical platform will reduce reliance on radioactive tracers and animal studies, advancing safer and more efficient pharmaceutical drug analysis.

Objective

Halogens occur in about 25 percent of approved small-molecule drugs and are key tracers in ADME studies, yet quantitative metabolite profiling still depends on radiolabeled compounds that are costly to synthesize and generate radioactive waste. This project will pioneer negative-ion ICP-MS for pharmaceutical halogen analysis, offering a radiolabel-free, structure-independent alternative that enables direct, sensitive detection of fluorine or bromine. The objectives are to: (1) establish validated methods for bulk quantification of F and Br in biological matrices; (2) develop HPLC–negative-ion ICP-MS for robust and quantitative metabolite profiling; and (3) deliver quantitative two-dimensional maps revealing the distribution of halogenated drugs in tissues at micrometer-size resolution via LA-ICP-MS using matrix-matched standards. The work will implement and optimize a pre-commercial prototype, characterize reagent backgrounds and spectral interferences, mitigate them using collision–reaction cell technology, and determine figures of merit—reporting limits of detection in absolute units (mass per sample for bulk ICP-MS, on-column mass for HPLC–ICP-MS, and areal LoD for LA-ICP-MS), with ng per g equivalents where appropriate—before applying the workflows to pharmaceutical samples with input from industry; performance targets will be refined after pilot tests in relevant matrices. Results will advance plasma spectrometry at bulk, molecular, and spatial levels, reduce reliance on radiotracers and the use of animals, and deliver the an end-to-end, radiolabel-free workflow for halogen quantification across bulk analysis, chemical speciation, and elemental mapping.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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(opens in new window) HORIZON-MSCA-2025-PF

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Coordinator

UNIVERSITEIT GENT
Net EU contribution

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€ 200 400,00
Address
SINT PIETERSNIEUWSTRAAT 25
9000 Gent
Belgium

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Region
Vlaams Gewest Prov. Oost-Vlaanderen Arr. Gent
Activity type
Higher or Secondary Education Establishments
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Total cost

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Partners (2)