Cel The flow karyotyping method allows quantification of chromosomal DNA by flow cytometry and thus analysis of chromosomal aberrations on chromosome suspensions. Amounts of data providing statistical significance can be collected in a short time and the approach allows accurate quantification of chromosomal DNA content and composition. The limitation of the method is the loss of the cellular level of analysis with the impossibility to detect low-frequency or heterogeneous events. The aim of this intracellular flow karyotyping project is improving the technology to extend the method to the analysis of karyotype aberrations from individual cells. A tool combining the statistical precision of flow cytometry with single cell karyotyping will be developed. This technology might be especially useful for the detection and quantification of heterogeneous abnormalities. Chromosomal changes of this type occur through ionising radiation exposure and are involved in karyotype instability and tumorigenesis. This approach will be investigated both for biological dosimetry purposes, especially in low-dose contexts (count of abnormal cells, count of abnormalities per cell) and for research purposes (karyotype instability, tumorigenesis). Preliminary results demonstrating the feasibility were obtained using hydrodynamic destruction of mitotic cells by capillary flow high gradient devices and monovariate (DNA quantification) flow karyotyping. This approach of cell membrane destruction will be optimised and alternative methods (particularly ultrasonic disintegration) developed. The intracellular staining method of chromosomes with DNA specific fluorochromes will be improved especially for dual parameter (DNA content and base pair composition quantification) intracellular flow karyotype analysis. The method will be adapted for modern serial flow cytometer systems (first step: partners' equipment). The development of new algorithms and computer programs for data interpretation is in progress. In parallel to the technical improvements pilot research using different human cell line models will be conducted to investigate the method's parameters. Program(-y) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Temat(-y) 42 - Physical Chemical Biology Zaproszenie do składania wniosków Data not available System finansowania Data not available Koordynator CEA - Commissariat à l'Energie Atomique Wkład UE Brak danych Adres Avenue du Général Leclerc 60-68 92265 Fontenay aux Roses Francja Zobacz na mapie Koszt całkowity Brak danych Uczestnicy (3) Sortuj alfabetycznie Sortuj według wkładu UE Rozwiń wszystko Zwiń wszystko Russian Academy of Sciences Rosja Wkład UE Brak danych Adres 117984 Moscow Zobacz na mapie Koszt całkowity Brak danych Russian Academy of Sciences Rosja Wkład UE Brak danych Adres 194064 St. Petersburg Zobacz na mapie Koszt całkowity Brak danych Universiteit van Amsterdam Niderlandy Wkład UE Brak danych Adres 1105 AZ Amsterdam Zobacz na mapie Koszt całkowity Brak danych
