Objective
Receptor tyrosine kinases (RTK) are involved in the regulation of cell proliferation, survival, differentiation and motility upon binding of growth factors. Disturbance of the normal balance between tyrosine kinase and Protein Tyrosine Phosphatase (PTP) activity results in aberrant tyrosine phosphorylation and has been implicated in the etiology of several human diseases, including cancer, diabetes and inflammation. Thus, it is important to understand how phosphorylation is regulated by members of both enzyme families. The objective of this proposal is to analyze the spatial regulation of Epidermal Growth Factor (EGF) signaling by PTPs in intact living cells. For this we propose to use quantitative fluorescence lifetime imaging microscopy (FLIM) in combination with functional genomic approaches. We will obtain information about which PTPs regulate the kinase activity of the EGF receptor, and the biochemical conectivity among PTP activities in response to EGF and other growth factors or hormones. Finally we will also investigate how PTP activities are regulated in response to EGF and where this happens in the cell. This spatio-temporal regulation of PTP activities will be studied by a novel approach in which enzyme-substrate complexes are directly imaged in living cells. Observation of `live´ biochemistry on a microscopic level has the advantage of preserving the network interconnectivity and spatial organization, allowing the investigation of the molecular dynamics that gives rise to the specificity in intracellular signal transduction. Since cellular responses are generated by the action of different gene products, cellular biology demands the regulation of signaling pathways at a proteomic scale to be studied to understand how signal specificity is achieved in cells and how stimuli are integrated to develop specific responses. Thus, our goal will be to obtain new information about dynamic properties of signaling networks at the proteomic scale.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences clinical medicine endocrinology diabetes
- natural sciences physical sciences optics microscopy fluorescence lifetime imaging
- medical and health sciences clinical medicine oncology
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-2-1-IEF
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.