Objective
Our knowledge on the marine nitrogen cycle has undergone some major revisions very recently with the discovery of novel pathways mediated by thus far unknown and partly unculturable prokaryotes. Among these new drivers of the nitrogen cycle are non-thermophilic Crenarchaea and Euryarchaea. Recently, Crenarchaea have been shown to harbor the amoA gene, responsible for the expression of ammonia monooxygenase, which represents a key enzyme in the nitrification step. The project aims at determining the dynamics in abundance and activity of these Archaea in European coastal seas. Specific emphasis is paid to differentiate between the free-living, planktonic Archaea and those attached to the two major types of suspended particles in coastal areas, lithogenic vs biogenic particles. We will assess the phylogenetic composition of the free-living and particle-attached crenarchaeal and euryarchaeal communities and compare them with those of beta- and gammaproteobacterial communities as these two bacterial groups are also known to harbor nitrifying phylotypes. Conventional fingerprinting techniques using the 16 S rRNA gene and terminal-restriction fragment length polymorphism, cloning and sequencing will be combined with the pyro-sequencing approach, the latter allowing an unsurpassed resolution of the total number of phylotypes present. Quantitative PCR on the 16S rRNA level and on crenarchaeal and beta- and gammaproteobacterial amoA gene level will be used to determine the number of crenarchaea and bacteria putatively oxidizing ammonia. This approach will be combined with fluorescence in situ hybridization and autoradiography to allow insights into the microspatial distribution of Crenarchaea on suspended particles. These molecular approaches to determine the abundance and phylogeny of nitrifiers in ambient water and on suspended particles are complemented by nitrification rate measurements differentiating, for the first time, bacterial from crenarchaeal nitrification using
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biological morphology comparative morphology
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2007-2-1-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
1010 Wien
Austria
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.