Release of engineered extracellular vesicles for delivery of biotherapeutics

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Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Injected extracellular vesicles and other nanoparticles hitchhike on erythrocytes and platelets from circulation towards organ clearance

Author(s): Svetlana Pavlova, Doste R. Mamand, Daniel W. Hagey, Xiuming Liang, H. Yesid Estupiñán, Wenyi Zheng, Guannan Zhou, Risul Amin, Antje M. Zickler, Scott Bonner-Harris, Zankruti Dave, Miina Ojansivu, Manuela O. Gustafsson, Oliver G. Hayes, Oscar P.B. Wiklander, Manuchehr Abedi‐Valugerdi, Samantha Roudi, Antonin Marquant, Joel Z. Nordin, Molly M. Stevens, André Görgens, Samir EL Andaloussi
Published in: Extracellular Vesicle, Issue 7, 2026, Page(s) 100103, ISSN 2773-0417
Publisher: Elsevier BV
DOI: 10.1016/j.vesic.2025.100103

Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase.

Author(s): Doste R Mamand, Safa Bazaz, Dara K. Mohammad, Osama Saher,Oscar P.B. Wiklander, Behnam Sadeghi, Moustapha Hassan, Samir EL-Andaloussi, Manuchehr Abedi-Valugerdi.
Published in: International immunopharmacology, Issue Vol. 129, 2024, Page(s) 111584, ISSN 1567-5769
Publisher: Elsevier BV
DOI: 10.1016/j.intimp.2024.111584

Surface display of functional moieties on extracellular vesicles using lipid anchors

Author(s): Wenyi Zheng, Melanie Schürz, Rim Jawad Wiklander, Oskar Gustafsson, Dhanu Gupta, Radka Slovak, Andrei Traista, Arianna Coluzzi, Samantha Roudi, Antonella Barone, Daria Farcas, Eleni Kyriakopoulou, Valentina Galli, Heena Sharma, Nicole Meisner-Kober, Malgorzata Honcharenko, Samir E.L. Andaloussi
Published in: Journal of Controlled Release, Issue 357, 2025, Page(s) 630-640, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2023.04.033

Engineering of extracellular vesicles for efficient intracellular delivery of multimodal therapeutics including genome editors

Author(s): Xiuming Liang, Dhanu Gupta, Junhua Xie, Elien Van Wonterghem, Lien Van Hoecke, Justin Hean, Zheyu Niu, Marziyeh Ghaeidamini, Oscar P. B. Wiklander, Wenyi Zheng, Rim Jawad Wiklander, Rui He, Doste R. Mamand, Jeremy Bost, Guannan Zhou, Houze Zhou, Samantha Roudi, H. Yesid Estupiñán, Julia Rädler, Antje M. Zickler, André Görgens, Vicky W. Q. Hou, Radka Slovak, Daniel W. Hagey, Olivier G. de Jong
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-025-59377-y

Development of Endogenous Protein Probes for Characterizing Surface Proteins and Cellular Interactors of Extracellular Vesicles

Author(s): Wenyi Zheng, Metoboroghene Mowoe, Wenqing Hou, Daniel W. Hagey, Koshi Imami, Samir EL Andaloussi
Published in: Advanced Science, Issue 13, 2026, ISSN 2198-3844
Publisher: Wiley
DOI: 10.1002/advs.202511414

Systemic Inflammation Modulates Clearance and Drives Extra‐Hepatic Distribution of Extracellular Vesicles

Author(s): Svetlana Pavlova, Doste R. Mamand, André Görgens, Antje M. Zickler, Wenyi Zheng, Xiuming Liang, Oscar P. B. Wiklander, Manuchehr Abedi‐Valugerdi, Elien Van Wonterghem, Junhua Xie, Zheyu Niu, Samantha Roudi, Dongnan Yan, Ruhani Makkar, Guannan Zhou, Koshi Imami, Matthew J. A Wood, Roosmarijn E. Vandenbroucke, Dhanu Gupta, Samir El Andaloussi
Published in: Journal of Extracellular Vesicles, Issue 15, 2026, ISSN 2001-3078
Publisher: Wiley
DOI: 10.1002/jev2.70256

Antibody-displaying extracellular vesicles for targeted cancer therapy

Author(s): Oscar P. B. Wiklander, Doste R. Mamand, Dara K. Mohammad, Wenyi Zheng, Rim Jawad Wiklander, Taras Sych, Antje M. Zickler, Xiuming Liang, Heena Sharma, Andrea Lavado, Jeremy Bost, Samantha Roudi, Giulia Corso, Angus J. Lennaárd, Manuchehr Abedi-Valugerdi, Imre Mäger, Evren Alici, Erdinc Sezgin, Joel Z. Nordin, Dhanu Gupta, André Görgens, Samir EL Andaloussi
Published in: Nature Biomedical Engineering, Issue 8, 2024, Page(s) 1453-1468, ISSN 2157-846X
Publisher: Springer Nature
DOI: 10.1038/s41551-024-01214-6

Genetic tools for investigating the life cycle of extracellular vesicles

Author(s): Wenyi Zheng, Samantha Roudi, Houze Zhou, Maribel Lara Corona, Guillaume van Niel, Joel Z. Nordin, Samir EL Andaloussi
Published in: Nature Reviews Bioengineering, Issue 3, 2025, Page(s) 505-520, ISSN 2731-6092
Publisher: Springer Nature
DOI: 10.1038/s44222-025-00286-6

The cellular response to extracellular vesicles is dependent on their cell source and dose

Author(s): Daniel W Hagey, Miina Ojansivu, Beklem R Bostancioglu, Osama Saher , Jeremy P Bost, Manuela O Gustafsson, Roberto Gramignoli, Mathias Svahn, Dhanu Gupta, Molly M Stevens , André Görgens, Samir El Andaloussi
Published in: Science Advances, Issue Vol 9 (35), 2023, Page(s) NA, ISSN 2375-2548
Publisher: AAAS
DOI: 10.1126/sciadv.adh1168

Formulation and Characterization of Novel Ionizable and Cationic Lipid Nanoparticles for the Delivery of Splice‐Switching Oligonucleotides

Author(s): Miina Ojansivu, Hanna M. G. Barriga, Margaret N. Holme, Stefanie Morf, James J. Doutch, Samir EL Andaloussi, Tomas Kjellman, Markus Johnsson, Justas Barauskas, Molly M. Stevens
Published in: Advanced Materials, Issue 37, 2025, ISSN 0935-9648
Publisher: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202419538

Identification of scaffold proteins for improved endogenous engineering of extracellular vesicles

Author(s): Wenyi Zheng, Julia Rädler, Helena Sork, Zheyu Zhe Niu, Samantha Roudi, Jeremy P Bost, André Görgens, Ying Zhao, Doste R Mamand, Xiuming Liang, Oscar P B Wiklander, Taavi Lehto, Dhanu Gupta, Joel Z Nordin, Samir El Andaloussi
Published in: Nature communications, Issue Vol.14 (1), 2023, Page(s) 4734-4734, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-023-40453-0

Novel Endogenous Engineering Platform for Robust Loading and Delivery of Functional mRNA by Extracellular Vesicles

Author(s): Antje M. Zickler, Xiuming Liang, Dhanu Gupta, Doste R. Mamand, Mariacristina De Luca, Giulia Corso, Lorenzo Errichelli, Justin Hean, Titash Sen, Omnia M. Elsharkasy, Noriyasu Kamei, Zheyu Niu, Guannan Zhou, Houze Zhou, Samantha Roudi, Oscar P. B. Wiklander, André Görgens, Joel Z. Nordin, Virginia Castilla‐Llorente, Samir EL Andaloussi
Published in: Advanced Science, Issue 11, 2025, ISSN 2198-3844
Publisher: Wiley
DOI: 10.1002/advs.202407619

Evaluation of Tetraspanins in Extracellular Vesicle Bioengineering

Author(s): Doste R. Mamand, Oskar Gustafsson, Helena Sork, Rim Jawad Wiklander, Safa Bazaz, Xiuming Liang, Vicky W. Q. Hou, Taavi Lehto, Dhanu Gupta, André Görgens, Joel Z. Nordin, Samir E. L. Andaloussi, Oscar P. B. Wiklander
Published in: Small Science, Issue 6, 2026, ISSN 2688-4046
Publisher: Wiley
DOI: 10.1002/smsc.202500533

Rolling Ultrasharp Microneedle Spheres Enable Topical Delivery of Biologics Through the Skin

Author(s): Theocharis Nikiforos Iordanidis, Argyris Spyrou, Samantha Roudi, Fredrik J. Swartling, Göran Stemme, Samir EL Andaloussi, Niclas Roxhed
Published in: Advanced Healthcare Materials, Issue 14, 2025, ISSN 2192-2640
Publisher: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202500990

Imaging Single Particle Profiler to Study Nanoscale Bioparticles Using Conventional Confocal Microscopy

Author(s): Taras Sych, André Görgens, Loïc Steiner, Gozde Gucluler, Ylva Huge, Farhood Alamdari, Markus Johansson, Firas Aljabery, Amir Sherif, Susanne Gabrielsson, Samir El Andaloussi, Erdinc Sezgin
Published in: Nano Letters, Issue 25, 2025, Page(s) 2173-2180, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.4c05117

Cell‐specific targeting of extracellular vesicles though engineering the glycocalyx

Author(s): Wenyi Zheng; Rui He; Xiuming Liang; Samantha Roudi; Jeremy Bost; Pierre‐Michael Coly; Guillaume van Niel; Samir E. L. Andaloussi
Published in: Journal of extracellular vesicles, Issue Vol.11 (12), 2022, Page(s) 12290-12290, ISSN 2001-3078
Publisher: United States: Wiley
DOI: 10.1002/jev2.12290

Extracellular vesicles engineered to bind albumin demonstrate extended circulation time and lymph node accumulation in mouse models

Author(s): Xiuming Liang,Zheyu Niu,Valentina Galli,Nathalie Howe,Ying Zhao,Oscar P. B. Wiklander,Wenyi Zheng,Rim Jawad Wiklander,Giulia Corso,Christopher Davies,Justin Hean,Eleni Kyriakopoulou,Doste R. Mamand,Risul Amin,Joel Z. Nordin,Dhanu Gupta,Samir EL Andaloussi
Published in: Journal of extracellular vesicles, Issue 20013078, 2022, ISSN 2001-3078
Publisher: Hoboken: John Wiley and Sons Inc
DOI: 10.1002/jev2.12248

Amelioration of systemic inflammation via the display of two different decoy protein receptors on extracellular vesicles

Author(s): Dhanu Gupta, Oscar P. B. Wiklander, André Görgens, Mariana Conceição, Giulia Corso, Xiuming Liang, Yiqi Seow, Sriram Balusu, Ulrika Feldin, Beklem Bostancioglu, Rim Jawad, Doste R. Mamand, Yi Xin Fiona Lee, Justin Hean, Imre Mäger, Thomas C. Roberts, Manuela Gustafsson, Dara K. Mohammad, Helena Sork, Alexandra Backlund, Per Lundin, Antonin de Fougerolles, C. I. Edvard Smith, Matthew J. A. Woo
Published in: Nature biomedical engineering, Issue Vol.5 (9), 2021, Page(s) 1084-1098, ISSN 2157-846X
Publisher: BERLIN: NATURE PORTFOLIO
DOI: 10.1038/s41551-021-00792-z

Multimeric Conjugates Using Engineered Peptide Scaffolds for Efficient siRNA Delivery

Author(s): Quentin Vicentini, Dennis Hekman, Deepak Bhatt, Rouven Stulz, Mahya Dezfouli, Peter Gennemark, Nicola Guzzi, Naoko Toki, Bojana Lazovic, Carolina Tängemo, Shalini Andersson, Samir EL Andaloussi, Anders Dahlén
Published in: Bioconjugate Chemistry, Issue 36, 2025, Page(s) 1299-1310, ISSN 1043-1802
Publisher: American Chemical Society
DOI: 10.1021/acs.bioconjchem.5c00156

Amphipathic Octenyl‐Alanine Modified Peptides Mediate Effective siRNA Delivery

Author(s): Tõnis Lehto, Marit Isakannu, Helena Sork, Annely Lorents, Safa Bazaz, Oscar P. B. Wiklander, Samir EL Andaloussi, Taavi Lehto
Published in: Journal of Peptide Science, Issue 31, 2025, ISSN 1075-2617
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/psc.70054

Extracellular vesicles originating from melanoma cells promote dysregulation in haematopoiesis as a component of cancer immunoediting

Author(s): Doste R. Mamand, Safa Bazaz, Dara K. Mohammad, Xiuming Liang, Svetlana Pavlova, Carsten Mim, Susanne Gabrielsson, Joel Z. Nordin, Oscar P. B. Wiklander, Manuchehr Abedi‐Valugerdi, Samir EL‐Andaloussi
Published in: Journal of Extracellular Vesicles, Issue 13, 2025, ISSN 2001-3078
Publisher: Wiley
DOI: 10.1002/jev2.12471

Bioengineered Extracellular Vesicles Mitigate Neuroinflammation by Neutralizing Pneumolysin and Delaying Disease Onset in Experimental Pneumococcal Meningitis

Author(s): Kristine Farmen, Doste R. Mamand, Miguel Tofiño‐Vian, Georgia Yfanti, Xiuming Liang, Houze Zhou, Vicky W. Q. Hou, Samir El Andaloussi, Oscar P. B. Wiklander, Federico Iovino
Published in: Journal of Extracellular Vesicles, Issue 15, 2026, ISSN 2001-3078
Publisher: Wiley
DOI: 10.1002/jev2.70369

Design and screening of novel endosomal escape compounds that enhance functional delivery of oligonucleotides in vitro

Author(s): H. Yesid Estupiñán, Tom Baladi, Samantha Roudi, Michael J. Munson, Jeremy Bost, Oskar Gustafsson, Daniel Velásquez-Ramírez, Deepak Kumar Bhatt, Daniel Hagey, Dennis Hekman, Shalini Andersson, Samir EL Andaloussi, Anders Dahlén
Published in: Molecular Therapy Nucleic Acids, Issue 36, 2025, Page(s) 102522, ISSN 2162-2531
Publisher: Nature Publishing Group
DOI: 10.1016/j.omtn.2025.102522

Optimised Electroporation for Loading of Extracellular Vesicles with Doxorubicin

Author(s): Angus J. Lennaárd, Doste R. Mamand, Rim Jawad Wiklander, Samir EL Andaloussi, Oscar P. B. Wiklander
Published in: Pharmaceutics, Issue 14, 2025, Page(s) 38, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics14010038

Identification of robust and abundant reference transcripts for EV mRNA cargo normalization

Author(s): Antje M. Zickler, Radosław Grochowski, André Görgens, Erik Bäcklin, Maximilian Kordes, J.-Matthias Löhr, Joel Z. Nordin, Samir EL Andaloussi, Daniel W. Hagey
Published in: Extracellular Vesicle, Issue 5, 2025, Page(s) 100065, ISSN 2773-0417
Publisher: Elsevier BV
DOI: 10.1016/j.vesic.2025.100065

Screening scaffold proteins for improved functional delivery of luminal proteins using engineered extracellular vesicles

Author(s): Zheyu Niu, Houze Zhou, Wenyi Zheng, Oliver G. Hayes, Vicky W.Q. Hou, André Görgens, Samantha Roudi, Guannan Zhou, Rim Jawad Wiklander, Taras Sych, Erdinc Sezgin, Joel Z. Nordin, Ying Zhao, Xiuming Liang, Samir E.L. Andaloussi
Published in: Journal of Controlled Release, Issue 384, 2026, Page(s) 113882, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2025.113882

Identification of storage conditions stabilizing extracellular vesicles preparations

Author(s): André Görgens,Giulia Corso,Daniel W. Hagey,Rim Jawad Wiklander,Manuela O. Gustafsson,Ulrika Felldin,Yi Lee,R. Beklem Bostancioglu,Helena Sork,Xiuming Liang,Wenyi Zheng,Dara K. Mohammad,Simonides I. van de Wakker,Pieter Vader,Antje M. Zickler,Doste R. Mamand,Li Ma,Margaret N. Holme,Molly M. Stevens,Oscar P. B. Wiklander,Samir EL Andaloussi
Published in: Journal of extracellular vesicles, Issue Vol.11 (6), 2022, ISSN 2001-3078
Publisher: Hoboken: John Wiley and Sons Inc
DOI: 10.1002/jev2.12238

Advanced peptide nanoparticles enable robust and efficient delivery of gene editors across cell types

Author(s): Oskar Gustafsson, Supriya Krishna, Sophia Borate, Marziyeh Ghaeidamini, Xiuming Liang, Osama Saher, Raul Cuellar, Björn K. Birdsong, Samantha Roudi, H. Yesid Estupiñán, Evren Alici, C.I. Edvard Smith, Elin K. Esbjörner, Simone Spuler, Olivier Gerrit de Jong, Helena Escobar, Joel Z. Nordin, Samir E.L. Andaloussi
Published in: Journal of Controlled Release, Issue 386, 2026, Page(s) 114038, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2025.114038

RNAi delivery platforms : bioconjugates and extracellular vesicles

Author(s): Julia Rädler
Published in: 2026, ISBN 978-91-8141-113-3
Publisher: Karolinska Institutet
DOI: 10.69622/31959393

In vivo and in situ delivery of biotherapeutics for improved extrahepatic bioavailability

Author(s): Samantha Roudi
Published in: 2026, ISBN 978-91-8141-150-8
Publisher: Karolinska Institutet
DOI: 10.69622/32315469

Enhancing the Efficiency of Viral and Non-viral Gene Delivery Vectors

Author(s): Safa Bazaz
Published in: 2024, ISBN 978-91-8017-276-9
Publisher: Karolinska Institutet

Exploiting the biogenesis of extracellular vesicles for bioengineering and therapeutic cargo loading

Author(s): Julia Rädler, Dhanu Gupta, Antje Zickler, Samir El Andaloussi
Published in: Molecular Therapy, Issue Vol. 31 (5), 2023, Page(s) 1231-1250
Publisher: Elsevier
DOI: 10.1016/j.ymthe.2023.02.013

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