Release of engineered extracellular vesicles for delivery of biotherapeutics

Información del proyecto

DELIVER

Identificador del acuerdo de subvención: 101001374

DOI

10.3030/101001374

Proyecto cerrado

Fecha de la firma de la CE 4 Febrero 2021

Fecha de inicio 1 Marzo 2021

Fecha de finalización 31 Agosto 2026

Financiado con arreglo a

• EXCELLENT SCIENCE - European Research Council (ERC)

Coste total

€ 2 000 000,00

Aportación de la UE

€ 2 000 000,00

Coordinado por

KAROLINSKA INSTITUTET
Sweden

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Publicaciones

Peer reviewed articles (25)

Injected extracellular vesicles and other nanoparticles hitchhike on erythrocytes and platelets from circulation towards organ clearance

Autores: Svetlana Pavlova, Doste R. Mamand, Daniel W. Hagey, Xiuming Liang, H. Yesid Estupiñán, Wenyi Zheng, Guannan Zhou, Risul Amin, Antje M. Zickler, Scott Bonner-Harris, Zankruti Dave, Miina Ojansivu, Manuela O. Gustafsson, Oliver G. Hayes, Oscar P.B. Wiklander, Manuchehr Abedi‐Valugerdi, Samantha Roudi, Antonin Marquant, Joel Z. Nordin, Molly M. Stevens, André Görgens, Samir EL Andaloussi
Publicado en: Extracellular Vesicle, Edición 7, 2026, Página(s) 100103, ISSN 2773-0417
Editor: Elsevier BV
DOI: 10.1016/j.vesic.2025.100103

Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase.

Autores: Doste R Mamand, Safa Bazaz, Dara K. Mohammad, Osama Saher,Oscar P.B. Wiklander, Behnam Sadeghi, Moustapha Hassan, Samir EL-Andaloussi, Manuchehr Abedi-Valugerdi.
Publicado en: International immunopharmacology, Edición Vol. 129, 2024, Página(s) 111584, ISSN 1567-5769
Editor: Elsevier BV
DOI: 10.1016/j.intimp.2024.111584

Engineering of extracellular vesicles for efficient intracellular delivery of multimodal therapeutics including genome editors

Autores: Xiuming Liang, Dhanu Gupta, Junhua Xie, Elien Van Wonterghem, Lien Van Hoecke, Justin Hean, Zheyu Niu, Marziyeh Ghaeidamini, Oscar P. B. Wiklander, Wenyi Zheng, Rim Jawad Wiklander, Rui He, Doste R. Mamand, Jeremy Bost, Guannan Zhou, Houze Zhou, Samantha Roudi, H. Yesid Estupiñán, Julia Rädler, Antje M. Zickler, André Görgens, Vicky W. Q. Hou, Radka Slovak, Daniel W. Hagey, Olivier G. de Jong
Publicado en: Nature Communications, Edición 16, 2025, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-025-59377-y

Development of Endogenous Protein Probes for Characterizing Surface Proteins and Cellular Interactors of Extracellular Vesicles

Autores: Wenyi Zheng, Metoboroghene Mowoe, Wenqing Hou, Daniel W. Hagey, Koshi Imami, Samir EL Andaloussi
Publicado en: Advanced Science, Edición 13, 2026, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202511414

Systemic Inflammation Modulates Clearance and Drives Extra‐Hepatic Distribution of Extracellular Vesicles

Autores: Svetlana Pavlova, Doste R. Mamand, André Görgens, Antje M. Zickler, Wenyi Zheng, Xiuming Liang, Oscar P. B. Wiklander, Manuchehr Abedi‐Valugerdi, Elien Van Wonterghem, Junhua Xie, Zheyu Niu, Samantha Roudi, Dongnan Yan, Ruhani Makkar, Guannan Zhou, Koshi Imami, Matthew J. A Wood, Roosmarijn E. Vandenbroucke, Dhanu Gupta, Samir El Andaloussi
Publicado en: Journal of Extracellular Vesicles, Edición 15, 2026, ISSN 2001-3078
Editor: Wiley
DOI: 10.1002/jev2.70256

Antibody-displaying extracellular vesicles for targeted cancer therapy

Autores: Oscar P. B. Wiklander, Doste R. Mamand, Dara K. Mohammad, Wenyi Zheng, Rim Jawad Wiklander, Taras Sych, Antje M. Zickler, Xiuming Liang, Heena Sharma, Andrea Lavado, Jeremy Bost, Samantha Roudi, Giulia Corso, Angus J. Lennaárd, Manuchehr Abedi-Valugerdi, Imre Mäger, Evren Alici, Erdinc Sezgin, Joel Z. Nordin, Dhanu Gupta, André Görgens, Samir EL Andaloussi
Publicado en: Nature Biomedical Engineering, Edición 8, 2024, Página(s) 1453-1468, ISSN 2157-846X
Editor: Springer Nature
DOI: 10.1038/s41551-024-01214-6

Genetic tools for investigating the life cycle of extracellular vesicles

Autores: Wenyi Zheng, Samantha Roudi, Houze Zhou, Maribel Lara Corona, Guillaume van Niel, Joel Z. Nordin, Samir EL Andaloussi
Publicado en: Nature Reviews Bioengineering, Edición 3, 2025, Página(s) 505-520, ISSN 2731-6092
Editor: Springer Nature
DOI: 10.1038/s44222-025-00286-6

The cellular response to extracellular vesicles is dependent on their cell source and dose

Autores: Daniel W Hagey, Miina Ojansivu, Beklem R Bostancioglu, Osama Saher , Jeremy P Bost, Manuela O Gustafsson, Roberto Gramignoli, Mathias Svahn, Dhanu Gupta, Molly M Stevens , André Görgens, Samir El Andaloussi
Publicado en: Science Advances, Edición Vol 9 (35), 2023, Página(s) NA, ISSN 2375-2548
Editor: AAAS
DOI: 10.1126/sciadv.adh1168

Formulation and Characterization of Novel Ionizable and Cationic Lipid Nanoparticles for the Delivery of Splice‐Switching Oligonucleotides

Autores: Miina Ojansivu, Hanna M. G. Barriga, Margaret N. Holme, Stefanie Morf, James J. Doutch, Samir EL Andaloussi, Tomas Kjellman, Markus Johnsson, Justas Barauskas, Molly M. Stevens
Publicado en: Advanced Materials, Edición 37, 2025, ISSN 0935-9648
Editor: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202419538

Identification of scaffold proteins for improved endogenous engineering of extracellular vesicles

Autores: Wenyi Zheng, Julia Rädler, Helena Sork, Zheyu Zhe Niu, Samantha Roudi, Jeremy P Bost, André Görgens, Ying Zhao, Doste R Mamand, Xiuming Liang, Oscar P B Wiklander, Taavi Lehto, Dhanu Gupta, Joel Z Nordin, Samir El Andaloussi
Publicado en: Nature communications, Edición Vol.14 (1), 2023, Página(s) 4734-4734, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-023-40453-0

Novel Endogenous Engineering Platform for Robust Loading and Delivery of Functional mRNA by Extracellular Vesicles

Autores: Antje M. Zickler, Xiuming Liang, Dhanu Gupta, Doste R. Mamand, Mariacristina De Luca, Giulia Corso, Lorenzo Errichelli, Justin Hean, Titash Sen, Omnia M. Elsharkasy, Noriyasu Kamei, Zheyu Niu, Guannan Zhou, Houze Zhou, Samantha Roudi, Oscar P. B. Wiklander, André Görgens, Joel Z. Nordin, Virginia Castilla‐Llorente, Samir EL Andaloussi
Publicado en: Advanced Science, Edición 11, 2025, ISSN 2198-3844
Editor: Wiley
DOI: 10.1002/advs.202407619

Evaluation of Tetraspanins in Extracellular Vesicle Bioengineering

Autores: Doste R. Mamand, Oskar Gustafsson, Helena Sork, Rim Jawad Wiklander, Safa Bazaz, Xiuming Liang, Vicky W. Q. Hou, Taavi Lehto, Dhanu Gupta, André Görgens, Joel Z. Nordin, Samir E. L. Andaloussi, Oscar P. B. Wiklander
Publicado en: Small Science, Edición 6, 2026, ISSN 2688-4046
Editor: Wiley
DOI: 10.1002/smsc.202500533

Rolling Ultrasharp Microneedle Spheres Enable Topical Delivery of Biologics Through the Skin

Autores: Theocharis Nikiforos Iordanidis, Argyris Spyrou, Samantha Roudi, Fredrik J. Swartling, Göran Stemme, Samir EL Andaloussi, Niclas Roxhed
Publicado en: Advanced Healthcare Materials, Edición 14, 2025, ISSN 2192-2640
Editor: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202500990

Imaging Single Particle Profiler to Study Nanoscale Bioparticles Using Conventional Confocal Microscopy

Autores: Taras Sych, André Görgens, Loïc Steiner, Gozde Gucluler, Ylva Huge, Farhood Alamdari, Markus Johansson, Firas Aljabery, Amir Sherif, Susanne Gabrielsson, Samir El Andaloussi, Erdinc Sezgin
Publicado en: Nano Letters, Edición 25, 2025, Página(s) 2173-2180, ISSN 1530-6984
Editor: American Chemical Society
DOI: 10.1021/acs.nanolett.4c05117

Cell‐specific targeting of extracellular vesicles though engineering the glycocalyx

Autores: Wenyi Zheng; Rui He; Xiuming Liang; Samantha Roudi; Jeremy Bost; Pierre‐Michael Coly; Guillaume van Niel; Samir E. L. Andaloussi
Publicado en: Journal of extracellular vesicles, Edición Vol.11 (12), 2022, Página(s) 12290-12290, ISSN 2001-3078
Editor: United States: Wiley
DOI: 10.1002/jev2.12290

Extracellular vesicles engineered to bind albumin demonstrate extended circulation time and lymph node accumulation in mouse models

Autores: Xiuming Liang,Zheyu Niu,Valentina Galli,Nathalie Howe,Ying Zhao,Oscar P. B. Wiklander,Wenyi Zheng,Rim Jawad Wiklander,Giulia Corso,Christopher Davies,Justin Hean,Eleni Kyriakopoulou,Doste R. Mamand,Risul Amin,Joel Z. Nordin,Dhanu Gupta,Samir EL Andaloussi
Publicado en: Journal of extracellular vesicles, Edición 20013078, 2022, ISSN 2001-3078
Editor: Hoboken: John Wiley and Sons Inc
DOI: 10.1002/jev2.12248

Amelioration of systemic inflammation via the display of two different decoy protein receptors on extracellular vesicles

Autores: Dhanu Gupta, Oscar P. B. Wiklander, André Görgens, Mariana Conceição, Giulia Corso, Xiuming Liang, Yiqi Seow, Sriram Balusu, Ulrika Feldin, Beklem Bostancioglu, Rim Jawad, Doste R. Mamand, Yi Xin Fiona Lee, Justin Hean, Imre Mäger, Thomas C. Roberts, Manuela Gustafsson, Dara K. Mohammad, Helena Sork, Alexandra Backlund, Per Lundin, Antonin de Fougerolles, C. I. Edvard Smith, Matthew J. A. Woo
Publicado en: Nature biomedical engineering, Edición Vol.5 (9), 2021, Página(s) 1084-1098, ISSN 2157-846X
Editor: BERLIN: NATURE PORTFOLIO
DOI: 10.1038/s41551-021-00792-z

Multimeric Conjugates Using Engineered Peptide Scaffolds for Efficient siRNA Delivery

Autores: Quentin Vicentini, Dennis Hekman, Deepak Bhatt, Rouven Stulz, Mahya Dezfouli, Peter Gennemark, Nicola Guzzi, Naoko Toki, Bojana Lazovic, Carolina Tängemo, Shalini Andersson, Samir EL Andaloussi, Anders Dahlén
Publicado en: Bioconjugate Chemistry, Edición 36, 2025, Página(s) 1299-1310, ISSN 1043-1802
Editor: American Chemical Society
DOI: 10.1021/acs.bioconjchem.5c00156

Amphipathic Octenyl‐Alanine Modified Peptides Mediate Effective siRNA Delivery

Autores: Tõnis Lehto, Marit Isakannu, Helena Sork, Annely Lorents, Safa Bazaz, Oscar P. B. Wiklander, Samir EL Andaloussi, Taavi Lehto
Publicado en: Journal of Peptide Science, Edición 31, 2025, ISSN 1075-2617
Editor: John Wiley & Sons Inc.
DOI: 10.1002/psc.70054

Bioengineered Extracellular Vesicles Mitigate Neuroinflammation by Neutralizing Pneumolysin and Delaying Disease Onset in Experimental Pneumococcal Meningitis

Autores: Kristine Farmen, Doste R. Mamand, Miguel Tofiño‐Vian, Georgia Yfanti, Xiuming Liang, Houze Zhou, Vicky W. Q. Hou, Samir El Andaloussi, Oscar P. B. Wiklander, Federico Iovino
Publicado en: Journal of Extracellular Vesicles, Edición 15, 2026, ISSN 2001-3078
Editor: Wiley
DOI: 10.1002/jev2.70369

Design and screening of novel endosomal escape compounds that enhance functional delivery of oligonucleotides in vitro

Autores: H. Yesid Estupiñán, Tom Baladi, Samantha Roudi, Michael J. Munson, Jeremy Bost, Oskar Gustafsson, Daniel Velásquez-Ramírez, Deepak Kumar Bhatt, Daniel Hagey, Dennis Hekman, Shalini Andersson, Samir EL Andaloussi, Anders Dahlén
Publicado en: Molecular Therapy Nucleic Acids, Edición 36, 2025, Página(s) 102522, ISSN 2162-2531
Editor: Nature Publishing Group
DOI: 10.1016/j.omtn.2025.102522

Identification of robust and abundant reference transcripts for EV mRNA cargo normalization

Autores: Antje M. Zickler, Radosław Grochowski, André Görgens, Erik Bäcklin, Maximilian Kordes, J.-Matthias Löhr, Joel Z. Nordin, Samir EL Andaloussi, Daniel W. Hagey
Publicado en: Extracellular Vesicle, Edición 5, 2025, Página(s) 100065, ISSN 2773-0417
Editor: Elsevier BV
DOI: 10.1016/j.vesic.2025.100065

Screening scaffold proteins for improved functional delivery of luminal proteins using engineered extracellular vesicles

Autores: Zheyu Niu, Houze Zhou, Wenyi Zheng, Oliver G. Hayes, Vicky W.Q. Hou, André Görgens, Samantha Roudi, Guannan Zhou, Rim Jawad Wiklander, Taras Sych, Erdinc Sezgin, Joel Z. Nordin, Ying Zhao, Xiuming Liang, Samir E.L. Andaloussi
Publicado en: Journal of Controlled Release, Edición 384, 2026, Página(s) 113882, ISSN 0168-3659
Editor: Elsevier BV
DOI: 10.1016/j.jconrel.2025.113882

Identification of storage conditions stabilizing extracellular vesicles preparations

Autores: André Görgens,Giulia Corso,Daniel W. Hagey,Rim Jawad Wiklander,Manuela O. Gustafsson,Ulrika Felldin,Yi Lee,R. Beklem Bostancioglu,Helena Sork,Xiuming Liang,Wenyi Zheng,Dara K. Mohammad,Simonides I. van de Wakker,Pieter Vader,Antje M. Zickler,Doste R. Mamand,Li Ma,Margaret N. Holme,Molly M. Stevens,Oscar P. B. Wiklander,Samir EL Andaloussi
Publicado en: Journal of extracellular vesicles, Edición Vol.11 (6), 2022, ISSN 2001-3078
Editor: Hoboken: John Wiley and Sons Inc
DOI: 10.1002/jev2.12238

Advanced peptide nanoparticles enable robust and efficient delivery of gene editors across cell types

Autores: Oskar Gustafsson, Supriya Krishna, Sophia Borate, Marziyeh Ghaeidamini, Xiuming Liang, Osama Saher, Raul Cuellar, Björn K. Birdsong, Samantha Roudi, H. Yesid Estupiñán, Evren Alici, C.I. Edvard Smith, Elin K. Esbjörner, Simone Spuler, Olivier Gerrit de Jong, Helena Escobar, Joel Z. Nordin, Samir E.L. Andaloussi
Publicado en: Journal of Controlled Release, Edición 386, 2026, Página(s) 114038, ISSN 0168-3659
Editor: Elsevier BV
DOI: 10.1016/j.jconrel.2025.114038

Thesis and dissertations (2)

RNAi delivery platforms : bioconjugates and extracellular vesicles

Autores: Julia Rädler
Publicado en: 2026, ISBN 978-91-8141-113-3
Editor: Karolinska Institutet
DOI: 10.69622/31959393

In vivo and in situ delivery of biotherapeutics for improved extrahepatic bioavailability

Autores: Samantha Roudi
Publicado en: 2026, ISBN 978-91-8141-150-8
Editor: Karolinska Institutet
DOI: 10.69622/32315469

Other (1)

Exploiting the biogenesis of extracellular vesicles for bioengineering and therapeutic cargo loading

Autores: Julia Rädler, Dhanu Gupta, Antje Zickler, Samir El Andaloussi
Publicado en: Molecular Therapy, Edición Vol. 31 (5), 2023, Página(s) 1231-1250
Editor: Elsevier
DOI: 10.1016/j.ymthe.2023.02.013