Release of engineered extracellular vesicles for delivery of biotherapeutics

Informazioni relative al progetto

DELIVER

ID dell’accordo di sovvenzione: 101001374

DOI

10.3030/101001374

Progetto chiuso

Data della firma CE 4 Febbraio 2021

Data di avvio 1 Marzo 2021

Data di completamento 31 Agosto 2026

Finanziato da

• EXCELLENT SCIENCE - European Research Council (ERC)

Costo totale

€ 2 000 000,00

Contributo UE

€ 2 000 000,00

Coordinato da

KAROLINSKA INSTITUTET
Sweden

CORDIS fornisce collegamenti ai risultati finali pubblici e alle pubblicazioni dei progetti ORIZZONTE.

I link ai risultati e alle pubblicazioni dei progetti del 7° PQ, così come i link ad alcuni tipi di risultati specifici come dataset e software, sono recuperati dinamicamente da .OpenAIRE .

Pubblicazioni

Peer reviewed articles (25)

Injected extracellular vesicles and other nanoparticles hitchhike on erythrocytes and platelets from circulation towards organ clearance

Autori: Svetlana Pavlova, Doste R. Mamand, Daniel W. Hagey, Xiuming Liang, H. Yesid Estupiñán, Wenyi Zheng, Guannan Zhou, Risul Amin, Antje M. Zickler, Scott Bonner-Harris, Zankruti Dave, Miina Ojansivu, Manuela O. Gustafsson, Oliver G. Hayes, Oscar P.B. Wiklander, Manuchehr Abedi‐Valugerdi, Samantha Roudi, Antonin Marquant, Joel Z. Nordin, Molly M. Stevens, André Görgens, Samir EL Andaloussi
Pubblicato in: Extracellular Vesicle, Numero 7, 2026, Pagina/e 100103, ISSN 2773-0417
Editore: Elsevier BV
DOI: 10.1016/j.vesic.2025.100103

Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase.

Autori: Doste R Mamand, Safa Bazaz, Dara K. Mohammad, Osama Saher,Oscar P.B. Wiklander, Behnam Sadeghi, Moustapha Hassan, Samir EL-Andaloussi, Manuchehr Abedi-Valugerdi.
Pubblicato in: International immunopharmacology, Numero Vol. 129, 2024, Pagina/e 111584, ISSN 1567-5769
Editore: Elsevier BV
DOI: 10.1016/j.intimp.2024.111584

Engineering of extracellular vesicles for efficient intracellular delivery of multimodal therapeutics including genome editors

Autori: Xiuming Liang, Dhanu Gupta, Junhua Xie, Elien Van Wonterghem, Lien Van Hoecke, Justin Hean, Zheyu Niu, Marziyeh Ghaeidamini, Oscar P. B. Wiklander, Wenyi Zheng, Rim Jawad Wiklander, Rui He, Doste R. Mamand, Jeremy Bost, Guannan Zhou, Houze Zhou, Samantha Roudi, H. Yesid Estupiñán, Julia Rädler, Antje M. Zickler, André Görgens, Vicky W. Q. Hou, Radka Slovak, Daniel W. Hagey, Olivier G. de Jong
Pubblicato in: Nature Communications, Numero 16, 2025, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-025-59377-y

Development of Endogenous Protein Probes for Characterizing Surface Proteins and Cellular Interactors of Extracellular Vesicles

Autori: Wenyi Zheng, Metoboroghene Mowoe, Wenqing Hou, Daniel W. Hagey, Koshi Imami, Samir EL Andaloussi
Pubblicato in: Advanced Science, Numero 13, 2026, ISSN 2198-3844
Editore: Wiley
DOI: 10.1002/advs.202511414

Systemic Inflammation Modulates Clearance and Drives Extra‐Hepatic Distribution of Extracellular Vesicles

Autori: Svetlana Pavlova, Doste R. Mamand, André Görgens, Antje M. Zickler, Wenyi Zheng, Xiuming Liang, Oscar P. B. Wiklander, Manuchehr Abedi‐Valugerdi, Elien Van Wonterghem, Junhua Xie, Zheyu Niu, Samantha Roudi, Dongnan Yan, Ruhani Makkar, Guannan Zhou, Koshi Imami, Matthew J. A Wood, Roosmarijn E. Vandenbroucke, Dhanu Gupta, Samir El Andaloussi
Pubblicato in: Journal of Extracellular Vesicles, Numero 15, 2026, ISSN 2001-3078
Editore: Wiley
DOI: 10.1002/jev2.70256

Antibody-displaying extracellular vesicles for targeted cancer therapy

Autori: Oscar P. B. Wiklander, Doste R. Mamand, Dara K. Mohammad, Wenyi Zheng, Rim Jawad Wiklander, Taras Sych, Antje M. Zickler, Xiuming Liang, Heena Sharma, Andrea Lavado, Jeremy Bost, Samantha Roudi, Giulia Corso, Angus J. Lennaárd, Manuchehr Abedi-Valugerdi, Imre Mäger, Evren Alici, Erdinc Sezgin, Joel Z. Nordin, Dhanu Gupta, André Görgens, Samir EL Andaloussi
Pubblicato in: Nature Biomedical Engineering, Numero 8, 2024, Pagina/e 1453-1468, ISSN 2157-846X
Editore: Springer Nature
DOI: 10.1038/s41551-024-01214-6

Genetic tools for investigating the life cycle of extracellular vesicles

Autori: Wenyi Zheng, Samantha Roudi, Houze Zhou, Maribel Lara Corona, Guillaume van Niel, Joel Z. Nordin, Samir EL Andaloussi
Pubblicato in: Nature Reviews Bioengineering, Numero 3, 2025, Pagina/e 505-520, ISSN 2731-6092
Editore: Springer Nature
DOI: 10.1038/s44222-025-00286-6

The cellular response to extracellular vesicles is dependent on their cell source and dose

Autori: Daniel W Hagey, Miina Ojansivu, Beklem R Bostancioglu, Osama Saher , Jeremy P Bost, Manuela O Gustafsson, Roberto Gramignoli, Mathias Svahn, Dhanu Gupta, Molly M Stevens , André Görgens, Samir El Andaloussi
Pubblicato in: Science Advances, Numero Vol 9 (35), 2023, Pagina/e NA, ISSN 2375-2548
Editore: AAAS
DOI: 10.1126/sciadv.adh1168

Formulation and Characterization of Novel Ionizable and Cationic Lipid Nanoparticles for the Delivery of Splice‐Switching Oligonucleotides

Autori: Miina Ojansivu, Hanna M. G. Barriga, Margaret N. Holme, Stefanie Morf, James J. Doutch, Samir EL Andaloussi, Tomas Kjellman, Markus Johnsson, Justas Barauskas, Molly M. Stevens
Pubblicato in: Advanced Materials, Numero 37, 2025, ISSN 0935-9648
Editore: United Nations Industrial Developement Organization
DOI: 10.1002/adma.202419538

Identification of scaffold proteins for improved endogenous engineering of extracellular vesicles

Autori: Wenyi Zheng, Julia Rädler, Helena Sork, Zheyu Zhe Niu, Samantha Roudi, Jeremy P Bost, André Görgens, Ying Zhao, Doste R Mamand, Xiuming Liang, Oscar P B Wiklander, Taavi Lehto, Dhanu Gupta, Joel Z Nordin, Samir El Andaloussi
Pubblicato in: Nature communications, Numero Vol.14 (1), 2023, Pagina/e 4734-4734, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-023-40453-0

Novel Endogenous Engineering Platform for Robust Loading and Delivery of Functional mRNA by Extracellular Vesicles

Autori: Antje M. Zickler, Xiuming Liang, Dhanu Gupta, Doste R. Mamand, Mariacristina De Luca, Giulia Corso, Lorenzo Errichelli, Justin Hean, Titash Sen, Omnia M. Elsharkasy, Noriyasu Kamei, Zheyu Niu, Guannan Zhou, Houze Zhou, Samantha Roudi, Oscar P. B. Wiklander, André Görgens, Joel Z. Nordin, Virginia Castilla‐Llorente, Samir EL Andaloussi
Pubblicato in: Advanced Science, Numero 11, 2025, ISSN 2198-3844
Editore: Wiley
DOI: 10.1002/advs.202407619

Evaluation of Tetraspanins in Extracellular Vesicle Bioengineering

Autori: Doste R. Mamand, Oskar Gustafsson, Helena Sork, Rim Jawad Wiklander, Safa Bazaz, Xiuming Liang, Vicky W. Q. Hou, Taavi Lehto, Dhanu Gupta, André Görgens, Joel Z. Nordin, Samir E. L. Andaloussi, Oscar P. B. Wiklander
Pubblicato in: Small Science, Numero 6, 2026, ISSN 2688-4046
Editore: Wiley
DOI: 10.1002/smsc.202500533

Rolling Ultrasharp Microneedle Spheres Enable Topical Delivery of Biologics Through the Skin

Autori: Theocharis Nikiforos Iordanidis, Argyris Spyrou, Samantha Roudi, Fredrik J. Swartling, Göran Stemme, Samir EL Andaloussi, Niclas Roxhed
Pubblicato in: Advanced Healthcare Materials, Numero 14, 2025, ISSN 2192-2640
Editore: John Wiley and Sons Ltd
DOI: 10.1002/adhm.202500990

Imaging Single Particle Profiler to Study Nanoscale Bioparticles Using Conventional Confocal Microscopy

Autori: Taras Sych, André Görgens, Loïc Steiner, Gozde Gucluler, Ylva Huge, Farhood Alamdari, Markus Johansson, Firas Aljabery, Amir Sherif, Susanne Gabrielsson, Samir El Andaloussi, Erdinc Sezgin
Pubblicato in: Nano Letters, Numero 25, 2025, Pagina/e 2173-2180, ISSN 1530-6984
Editore: American Chemical Society
DOI: 10.1021/acs.nanolett.4c05117

Cell‐specific targeting of extracellular vesicles though engineering the glycocalyx

Autori: Wenyi Zheng; Rui He; Xiuming Liang; Samantha Roudi; Jeremy Bost; Pierre‐Michael Coly; Guillaume van Niel; Samir E. L. Andaloussi
Pubblicato in: Journal of extracellular vesicles, Numero Vol.11 (12), 2022, Pagina/e 12290-12290, ISSN 2001-3078
Editore: United States: Wiley
DOI: 10.1002/jev2.12290

Extracellular vesicles engineered to bind albumin demonstrate extended circulation time and lymph node accumulation in mouse models

Autori: Xiuming Liang,Zheyu Niu,Valentina Galli,Nathalie Howe,Ying Zhao,Oscar P. B. Wiklander,Wenyi Zheng,Rim Jawad Wiklander,Giulia Corso,Christopher Davies,Justin Hean,Eleni Kyriakopoulou,Doste R. Mamand,Risul Amin,Joel Z. Nordin,Dhanu Gupta,Samir EL Andaloussi
Pubblicato in: Journal of extracellular vesicles, Numero 20013078, 2022, ISSN 2001-3078
Editore: Hoboken: John Wiley and Sons Inc
DOI: 10.1002/jev2.12248

Amelioration of systemic inflammation via the display of two different decoy protein receptors on extracellular vesicles

Autori: Dhanu Gupta, Oscar P. B. Wiklander, André Görgens, Mariana Conceição, Giulia Corso, Xiuming Liang, Yiqi Seow, Sriram Balusu, Ulrika Feldin, Beklem Bostancioglu, Rim Jawad, Doste R. Mamand, Yi Xin Fiona Lee, Justin Hean, Imre Mäger, Thomas C. Roberts, Manuela Gustafsson, Dara K. Mohammad, Helena Sork, Alexandra Backlund, Per Lundin, Antonin de Fougerolles, C. I. Edvard Smith, Matthew J. A. Woo
Pubblicato in: Nature biomedical engineering, Numero Vol.5 (9), 2021, Pagina/e 1084-1098, ISSN 2157-846X
Editore: BERLIN: NATURE PORTFOLIO
DOI: 10.1038/s41551-021-00792-z

Multimeric Conjugates Using Engineered Peptide Scaffolds for Efficient siRNA Delivery

Autori: Quentin Vicentini, Dennis Hekman, Deepak Bhatt, Rouven Stulz, Mahya Dezfouli, Peter Gennemark, Nicola Guzzi, Naoko Toki, Bojana Lazovic, Carolina Tängemo, Shalini Andersson, Samir EL Andaloussi, Anders Dahlén
Pubblicato in: Bioconjugate Chemistry, Numero 36, 2025, Pagina/e 1299-1310, ISSN 1043-1802
Editore: American Chemical Society
DOI: 10.1021/acs.bioconjchem.5c00156

Amphipathic Octenyl‐Alanine Modified Peptides Mediate Effective siRNA Delivery

Autori: Tõnis Lehto, Marit Isakannu, Helena Sork, Annely Lorents, Safa Bazaz, Oscar P. B. Wiklander, Samir EL Andaloussi, Taavi Lehto
Pubblicato in: Journal of Peptide Science, Numero 31, 2025, ISSN 1075-2617
Editore: John Wiley & Sons Inc.
DOI: 10.1002/psc.70054

Bioengineered Extracellular Vesicles Mitigate Neuroinflammation by Neutralizing Pneumolysin and Delaying Disease Onset in Experimental Pneumococcal Meningitis

Autori: Kristine Farmen, Doste R. Mamand, Miguel Tofiño‐Vian, Georgia Yfanti, Xiuming Liang, Houze Zhou, Vicky W. Q. Hou, Samir El Andaloussi, Oscar P. B. Wiklander, Federico Iovino
Pubblicato in: Journal of Extracellular Vesicles, Numero 15, 2026, ISSN 2001-3078
Editore: Wiley
DOI: 10.1002/jev2.70369

Design and screening of novel endosomal escape compounds that enhance functional delivery of oligonucleotides in vitro

Autori: H. Yesid Estupiñán, Tom Baladi, Samantha Roudi, Michael J. Munson, Jeremy Bost, Oskar Gustafsson, Daniel Velásquez-Ramírez, Deepak Kumar Bhatt, Daniel Hagey, Dennis Hekman, Shalini Andersson, Samir EL Andaloussi, Anders Dahlén
Pubblicato in: Molecular Therapy Nucleic Acids, Numero 36, 2025, Pagina/e 102522, ISSN 2162-2531
Editore: Nature Publishing Group
DOI: 10.1016/j.omtn.2025.102522

Identification of robust and abundant reference transcripts for EV mRNA cargo normalization

Autori: Antje M. Zickler, Radosław Grochowski, André Görgens, Erik Bäcklin, Maximilian Kordes, J.-Matthias Löhr, Joel Z. Nordin, Samir EL Andaloussi, Daniel W. Hagey
Pubblicato in: Extracellular Vesicle, Numero 5, 2025, Pagina/e 100065, ISSN 2773-0417
Editore: Elsevier BV
DOI: 10.1016/j.vesic.2025.100065

Screening scaffold proteins for improved functional delivery of luminal proteins using engineered extracellular vesicles

Autori: Zheyu Niu, Houze Zhou, Wenyi Zheng, Oliver G. Hayes, Vicky W.Q. Hou, André Görgens, Samantha Roudi, Guannan Zhou, Rim Jawad Wiklander, Taras Sych, Erdinc Sezgin, Joel Z. Nordin, Ying Zhao, Xiuming Liang, Samir E.L. Andaloussi
Pubblicato in: Journal of Controlled Release, Numero 384, 2026, Pagina/e 113882, ISSN 0168-3659
Editore: Elsevier BV
DOI: 10.1016/j.jconrel.2025.113882

Identification of storage conditions stabilizing extracellular vesicles preparations

Autori: André Görgens,Giulia Corso,Daniel W. Hagey,Rim Jawad Wiklander,Manuela O. Gustafsson,Ulrika Felldin,Yi Lee,R. Beklem Bostancioglu,Helena Sork,Xiuming Liang,Wenyi Zheng,Dara K. Mohammad,Simonides I. van de Wakker,Pieter Vader,Antje M. Zickler,Doste R. Mamand,Li Ma,Margaret N. Holme,Molly M. Stevens,Oscar P. B. Wiklander,Samir EL Andaloussi
Pubblicato in: Journal of extracellular vesicles, Numero Vol.11 (6), 2022, ISSN 2001-3078
Editore: Hoboken: John Wiley and Sons Inc
DOI: 10.1002/jev2.12238

Advanced peptide nanoparticles enable robust and efficient delivery of gene editors across cell types

Autori: Oskar Gustafsson, Supriya Krishna, Sophia Borate, Marziyeh Ghaeidamini, Xiuming Liang, Osama Saher, Raul Cuellar, Björn K. Birdsong, Samantha Roudi, H. Yesid Estupiñán, Evren Alici, C.I. Edvard Smith, Elin K. Esbjörner, Simone Spuler, Olivier Gerrit de Jong, Helena Escobar, Joel Z. Nordin, Samir E.L. Andaloussi
Pubblicato in: Journal of Controlled Release, Numero 386, 2026, Pagina/e 114038, ISSN 0168-3659
Editore: Elsevier BV
DOI: 10.1016/j.jconrel.2025.114038

Thesis and dissertations (2)

RNAi delivery platforms : bioconjugates and extracellular vesicles

Autori: Julia Rädler
Pubblicato in: 2026, ISBN 978-91-8141-113-3
Editore: Karolinska Institutet
DOI: 10.69622/31959393

In vivo and in situ delivery of biotherapeutics for improved extrahepatic bioavailability

Autori: Samantha Roudi
Pubblicato in: 2026, ISBN 978-91-8141-150-8
Editore: Karolinska Institutet
DOI: 10.69622/32315469

Other (1)

Exploiting the biogenesis of extracellular vesicles for bioengineering and therapeutic cargo loading

Autori: Julia Rädler, Dhanu Gupta, Antje Zickler, Samir El Andaloussi
Pubblicato in: Molecular Therapy, Numero Vol. 31 (5), 2023, Pagina/e 1231-1250
Editore: Elsevier
DOI: 10.1016/j.ymthe.2023.02.013