Novel mechanisms of adaptive and innate bacteriophage immunity

Project Information

DEFEND

Grant agreement ID: 101003229

DOI

10.3030/101003229

EC signature date 4 February 2021

Start date 1 June 2021

End date 31 May 2026

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 2 000 000,00

EU contribution

€ 2 000 000,00

Coordinated by

TECHNISCHE UNIVERSITEIT DELFT
Netherlands

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (15)

Modulating CRISPR-Cas genome editing using guide-complementary DNA oligonucleotides

Author(s): Thomas Swartjes; Peng Shang; Dennis van den Berg; Tim A. Künne; Niels Geijsen; Stan J.J. Brouns; John van der Oost; Raymond H.J. Staals; Richard A. Notebaart
Published in: The CRISPR Journal, 2022, ISSN 2573-1599
Publisher: Mary Ann Liebert
DOI: 10.1101/2022.01.15.475214

Structural basis for broad anti-phage immunity by DISARM

Author(s): Jack P. K. Bravo; Cristian Aparicio-Maldonado; Franklin L. Nobrega; Stan J. J. Brouns; David W. Taylor
Published in: Nature Communications, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1101/2021.12.26.474123

Phage tRNAs evade tRNA-targeting host defenses through anticodon loop mutations

Author(s): Daan F. van den Berg; Baltus A. van der Steen; Ana Rita Costa; Stan J. J. Brouns
Published in: eLife, 2023, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.21203/rs.3.rs-2166710/v1

Craspase is a CRISPR RNA-guided, RNA-activated protease

Author(s): Chunyi Hu; Sam P. B. van Beljouw; Ki Hyun Nam; Gabriel Schuler; Fran Ding; Yanru Cui; Alicia Rodríguez-Molina; Anna C. Haagsma; Menno Valk; Martin Pabst; Stan J. J. Brouns; Ailong Ke
Published in: Science, 2022, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.add5064

Single cell variability of CRISPR‐Cas interference and adaptation

Author(s): Rebecca E McKenzie, Emma M Keizer, Jochem N A Vink, Jasper van Lopik, Ferhat Büke, Vera Kalkman, Christian Fleck, Sander J Tans, Stan J J Brouns
Published in: Molecular Systems Biology, 2022, ISSN 1744-4292
Publisher: Nature Publishing Group
DOI: 10.15252/msb.202110680

An OmpW-dependent T4-like phage infects Serratia sp. ATCC 39006

Author(s): Marina Mahler; Lucia M. Malone; Daan F. van den Berg; Leah M. Smith; Stan J. J. Brouns; Peter C. Fineran
Published in: Microbial Genomics, 2023, ISSN 2057-5858
Publisher: London Society for General Microbiology
DOI: 10.1099/mgen.0.000968

Short prokaryotic Argonaute systems trigger cell death upon detection of invading DNA

Author(s): Balwina Koopal; Ana Potocnik; Sumanth K. Mutte; Cristian Aparicio-Maldonado; Simon Lindhoud; Jacques J.M. Vervoort; Stan J.J. Brouns; Daan C. Swarts
Published in: Cell, 2022, ISSN 1097-4172
Publisher: Cell press
DOI: 10.1016/j.cell.2022.03.012

Mechanisms and clinical importance of bacteriophage resistance.

Author(s): Julia E Egido; Ana Rita Costa; Ana Rita Costa; Cristian Aparicio-Maldonado; Cristian Aparicio-Maldonado; Pieter-Jan A. Haas; Stan J. J. Brouns; Stan J. J. Brouns
Published in: FEMS Microbiology Reviews, 2022, ISSN 1574-6976
Publisher: Elsevier
DOI: 10.1093/femsre/fuab048

CasPEDIA Database: a functional classification system for class 2 CRISPR-Cas enzymes

Author(s): Benjamin A Adler; Marena I Trinidad; Daniel Bellieny-Rabelo; Elaine Zhang; Hannah M Karp; Petr Skopintsev; Brittney W Thornton; Rachel F Weissman; Peter H Yoon; LinXing Chen; Tomas Hessler; Amy R Eggers; David Colognori; Ron Boger; Erin E Doherty; Connor A Tsuchida; Ryan V Tran; Laura Hofman; Honglue Shi; Kevin M Wasko; Zehan Zhou; Chenglong Xia; Muntathar J Al-Shimary; Jaymin R Patel; Vienna C J
Published in: Nucleic Acids Research, 2023, ISSN 1362-4962
Publisher: Oxford University Press
DOI: 10.1093/nar/gkad890

The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase.

Author(s): Sam P. B. van Beljouw; Sam P. B. van Beljouw; Anna C. Haagsma; Anna C. Haagsma; Alicia Rodríguez-Molina; Alicia Rodríguez-Molina; Daan F. van den Berg; Daan F. van den Berg; Jochem N.A. Vink; Jochem N.A. Vink; Stan J. J. Brouns; Stan J. J. Brouns
Published in: Science, 2021, ISSN 0036-8075
Publisher: American Association for the Advancement of Science
DOI: 10.1126/science.abk2718

Adaptation by Type V-A and V-B CRISPR-Cas Systems Demonstrates Conserved Protospacer Selection Mechanisms Between Diverse CRISPR-Cas Types

Author(s): Wen Y. Wu; Simon A. Jackson; Cristóbal Almendros; Anna C. Haagsma; Suzan Yilmaz; Gerrit Gort; John van der Oost; Stan J.J. Brouns; Raymond H.J. Staals
Published in: The CRISPR Journal, 2022, ISSN 2573-1602
Publisher: Mary Ann Liebert
DOI: 10.1089/crispr.2021.0150

PAM-repeat associations and spacer selection preferences in single and co-occurring CRISPR-Cas systems.

Author(s): Jochem N.A. Vink; Jochem N.A. Vink; Jan H. L. Baijens; Jan H. L. Baijens; Stan J. J. Brouns; Stan J. J. Brouns
Published in: Genome Biology, 2021, ISSN 1474-760X
Publisher: London BioMed Central
DOI: 10.1186/s13059-021-02495-9

Monitoring phage-induced lysis of Gram-negatives in real time using a fluorescent DNA dye

Author(s): Julia E. Egido; Catherine Toner-Bartelds; Ana Rita Costa; Stan J. J. Brouns; Suzan H. M. Rooijakkers; Bart W. Bardoel; Pieter-Jan Haas
Published in: Scientific Reports, 2023, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-023-27734-w

High-resolution reconstruction of a Jumbo-bacteriophage infecting capsulated bacteria using hyperbranched tail fibers

Author(s): Ruochen Ouyang; Ana Rita Costa; C. Keith Cassidy; Aleksandra Otwinowska; Vera C. J. Williams; Agnieszka Latka; Phill J. Stansfeld; Zuzanna Drulis-Kawa; Yves Briers; Daniël M. Pelt; Stan J. J. Brouns; Ariane Briegel
Published in: Nature Communications, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-34972-5

Accumulation of defense systems in phage-resistant strains of Pseudomonas aeruginosa

Author(s): Costa AR, van den Berg DF, Esser JQ, Muralidharan A, van den Bossche H, Bonilla BE, van der Steen BA, Haagsma AC, Fluit AC, Nobrega FL, Haas PJ, Brouns SJJ
Published in: Science Advances, 2024, ISSN 2375-2548
Publisher: Washington, DC American Association for the Advancement of Science
DOI: 10.1126/sciadv.adj0341